EC 2.3.3.13: 2-isopropylmalate synthase

TransferasesOpen in Browse

CatLog read 72 records for EC 2.3.3.13 from 7 papers, for 4 organisms. Names in these papers: α-isopropylmalate synthase (MtIPMS), α-isopropylmalate synthase, α-isopropylmalate synthase (MtuIPMS), α-isopropylmalate synthase (NmeIPMS) and 5 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 49 records for EC 2.3.3.13 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.3.3.13 in Browse.

Papers

Papers with records for EC 2.3.3.13
Paper	Organisms	Records
Kinetic and chemical mechanism of alpha-isopropylmalate synthase from Mycobacterium tuberculosis	Mycobacterium tuberculosis	13
Kinetic evidence for interdomain communication in the allosteric regulation of alpha-isopropylmalate synthase from Mycobacterium tuberculosis	Mycobacterium tuberculosis	13
Kinetic analysis of the effects of monovalent cations and divalent metals on the activity of Mycobacterium tuberculosis alpha-isopropylmalate synthase	Mycobacterium tuberculosis	10
The C-terminal regulatory domain is required for catalysis by Neisseria meningitidis alpha-isopropylmalate synthase	Neisseria meningitidis	10
Amino-acid substitutions at the domain interface affect substrate and allosteric inhibitor binding in α-isopropylmalate synthase from Mycobacterium tuberculosis	Mycobacterium tuberculosis	10
Isopropylmalate synthase regulatory domain removal abolishes feedback regulation at the expense of leucine homeostasis in plants	Brassica juncea	8
Amenability of the Gatekeeper Enzyme HphA to Engineering in the Homologation Pathway of l‑Phenylalanine and l‑Tyrosine through Homology-Based Site-Directed Mutagenesis	Nostoc punctiforme	8

Records

Records for EC 2.3.3.13 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
Bju.IPMS1-A1	Brassica juncea	3-methyl-2-oxobutanoate	20.37	0.1577	129.1		PMID 39869449	Open record: Bju.IPMS1-A1, 3-methyl-2-oxobutanoate
Bju.IPMS1-A1	Brassica juncea	acetyl-CoA	24.92	0.77	32.36		PMID 39869449	Open record: Bju.IPMS1-A1, acetyl-CoA
Bju.IPMS1-A2	Brassica juncea	3-methyl-2-oxobutanoate	20.52	0.1439	142.6		PMID 39869449	Open record: Bju.IPMS1-A2, 3-methyl-2-oxobutanoate
Bju.IPMS1-A2	Brassica juncea	acetyl-CoA	16.87	0.406	41.55		PMID 39869449	Open record: Bju.IPMS1-A2, acetyl-CoA
Bju.IPMS1-B1	Brassica juncea	3-methyl-2-oxobutanoate	24.22	0.2166	111.8		PMID 39869449	Open record: Bju.IPMS1-B1, 3-methyl-2-oxobutanoate
Bju.IPMS1-B1	Brassica juncea	acetyl-CoA	42.07	1.262	33.34		PMID 39869449	Open record: Bju.IPMS1-B1, acetyl-CoA
Bju.IPMS1-B2	Brassica juncea	3-methyl-2-oxobutanoate	16.74	0.2307	72.5		PMID 39869449	Open record: Bju.IPMS1-B2, 3-methyl-2-oxobutanoate
Bju.IPMS1-B2	Brassica juncea	acetyl-CoA	12.81	0.532	24.07		PMID 39869449	Open record: Bju.IPMS1-B2, acetyl-CoA
HphA (2-benzylmalate synthase)	Nostoc punctiforme	3-methyl-2-oxobutanoic acid	0.0078	4	0.0019	pH 7.5, 20.6 °C	PMID 41799147	Open record: HphA (2-benzylmalate synthase), 3-methyl-2-oxobutanoic acid
HphA (2-benzylmalate synthase)	Nostoc punctiforme	4-methyl-2-oxovaleric acid	—	—	0.0034	pH 7.5, 20.6 °C	PMID 41799147	Open record: HphA (2-benzylmalate synthase), 4-methyl-2-oxovaleric acid
HphA (2-benzylmalate synthase)variant A73L	Nostoc punctiforme	3-methyl-2-oxobutanoic acid	0.12	0.65	0.18	pH 7.5, 20.6 °C	PMID 41799147	Open record: HphA (2-benzylmalate synthase) variant A73L, 3-methyl-2-oxobutanoic acid
HphA (2-benzylmalate synthase)variant A73L	Nostoc punctiforme	4-hydroxyphenylpyruvic acid	0.22	0.41	0.54	pH 7.5, 20.6 °C	PMID 41799147	Open record: HphA (2-benzylmalate synthase) variant A73L, 4-hydroxyphenylpyruvic acid
HphA (2-benzylmalate synthase)variant A73L	Nostoc punctiforme	4-methyl-2-oxovaleric acid	0.1	1.6	0.064	pH 7.5, 20.6 °C	PMID 41799147	Open record: HphA (2-benzylmalate synthase) variant A73L, 4-methyl-2-oxovaleric acid
HphA (2-benzylmalate synthase)variant A73L	Nostoc punctiforme	hydroxypyruvic acid	0.0046	3.2	0.0014	pH 7.5, 20.6 °C	PMID 41799147	Open record: HphA (2-benzylmalate synthase) variant A73L, hydroxypyruvic acid
HphA (2-benzylmalate synthase)variant A73L	Nostoc punctiforme	indole-3-pyruvic acid	0.0091	0.21	0.043	pH 7.5, 20.6 °C	PMID 41799147	Open record: HphA (2-benzylmalate synthase) variant A73L, indole-3-pyruvic acid
HphA (2-benzylmalate synthase)variant A73L	Nostoc punctiforme	oxaloacetic acid	0.0069	—	—	pH 7.5, 20.6 °C	PMID 41799147	Open record: HphA (2-benzylmalate synthase) variant A73L, oxaloacetic acid
α-isopropylmalate synthase	Mycobacterium tuberculosis	acetyl-Coenzyme A20 mM KCl and 20 mM MgCl2 (Methods standard assay); Table 1	2.1	0.136	15 calc.	pH 7.4, 25 °C	PMID 16846242	Open record: α-isopropylmalate synthase, acetyl-Coenzyme A
α-isopropylmalate synthase	Mycobacterium tuberculosis	acetyl-Coenzyme A20 mM KCl and 20 mM MgCl2 (Methods standard assay); Table 1	5	0.279	18 calc.	pH 7.4, 25 °C	PMID 16846242	Open record: α-isopropylmalate synthase, acetyl-Coenzyme A
α-isopropylmalate synthase	Mycobacterium tuberculosis	acetyl-Coenzyme A20 mM KCl and 20 mM MgCl2 (Methods standard assay); Table 1	7.6	0.057	130 calc.	pH 7.4, 25 °C	PMID 16846242	Open record: α-isopropylmalate synthase, acetyl-Coenzyme A
α-isopropylmalate synthase	Mycobacterium tuberculosis	acetyl-Coenzyme A20 mM KCl and 20 mM MgCl2 (Methods standard assay); Table 1	4.5	0.074	61 calc.	pH 7.4, 25 °C	PMID 16846242	Open record: α-isopropylmalate synthase, acetyl-Coenzyme A
α-isopropylmalate synthase	Mycobacterium tuberculosis	acetyl-Coenzyme Awithout added MgCl2; Table 2	0.06	0.37	0.15	pH 7.4, 25 °C	PMID 16846242	Open record: α-isopropylmalate synthase, acetyl-Coenzyme A
α-isopropylmalate synthase	Mycobacterium tuberculosis	acetyl-Coenzyme Awith added MgCl2; Table 2	0.03	0.16	0.17	pH 7.4, 25 °C	PMID 16846242	Open record: α-isopropylmalate synthase, acetyl-Coenzyme A
α-isopropylmalate synthase	Mycobacterium tuberculosis	crotonyl-Coenzyme Awithout added MgCl2	0.05	0.05	0.94	pH 7.4, 25 °C	PMID 16846242	Open record: α-isopropylmalate synthase, crotonyl-Coenzyme A
α-isopropylmalate synthase	Mycobacterium tuberculosis	crotonyl-Coenzyme Awith added MgCl2	0.04	0.095	0.4	pH 7.4, 25 °C	PMID 16846242	Open record: α-isopropylmalate synthase, crotonyl-Coenzyme A
α-isopropylmalate synthase	Mycobacterium tuberculosis	propionyl-Coenzyme A	0.04	0.22	0.19	pH 7.4, 25 °C	PMID 16846242	Open record: α-isopropylmalate synthase, propionyl-Coenzyme A
α-isopropylmalate synthase	Mycobacterium tuberculosis	pyruvate	6.1	9.5	0.64 calc.	pH 7.4, 25 °C	PMID 16846242	Open record: α-isopropylmalate synthase, pyruvate
α-isopropylmalate synthase	Mycobacterium tuberculosis	α-ketobutyrate	10.7	0.86	12 calc.	pH 7.4, 25 °C	PMID 16846242	Open record: α-isopropylmalate synthase, α-ketobutyrate
α-isopropylmalate synthase	Mycobacterium tuberculosis	α-ketoisovalerate	3.5	0.012	290 calc.	pH 7.4, 25 °C	PMID 16846242	Open record: α-isopropylmalate synthase, α-ketoisovalerate
α-isopropylmalate synthase	Mycobacterium tuberculosis	α-ketovalerate	7	0.41	17 calc.	pH 7.4, 25 °C	PMID 16846242	Open record: α-isopropylmalate synthase, α-ketovalerate
α-isopropylmalate synthase (MtIPMS)	Mycobacterium tuberculosis	Acetyl Coenzyme ATable 4 monovalent-cation block: Me+ = K+ (saturating)	—	0.103	34	pH 7.4, 25 °C	PMID 16684501	Open record: α-isopropylmalate synthase (MtIPMS), Acetyl Coenzyme A
α-isopropylmalate synthase (MtIPMS)	Mycobacterium tuberculosis	Acetyl Coenzyme ATable 4 monovalent-cation block: Me+ = Rb+ (saturating)	—	0.12	28	pH 7.4, 25 °C	PMID 16684501	Open record: α-isopropylmalate synthase (MtIPMS), Acetyl Coenzyme A
α-isopropylmalate synthase (MtIPMS)	Mycobacterium tuberculosis	Acetyl Coenzyme ATable 4 divalent-metal block: Me2+ = Mg2+ (saturating)	—	0.138	15	pH 7.4, 25 °C	PMID 16684501	Open record: α-isopropylmalate synthase (MtIPMS), Acetyl Coenzyme A
α-isopropylmalate synthase (MtIPMS)	Mycobacterium tuberculosis	Acetyl Coenzyme ATable 4 divalent-metal block: Me2+ = Mn2+ (saturating)	—	0.05	34	pH 7.4, 25 °C	PMID 16684501	Open record: α-isopropylmalate synthase (MtIPMS), Acetyl Coenzyme A
α-isopropylmalate synthase (MtIPMS)	Mycobacterium tuberculosis	Acetyl Coenzyme ATable 4 divalent-metal block: Me2+ = Co2+ (saturating)	—	0.037	25	pH 7.4, 25 °C	PMID 16684501	Open record: α-isopropylmalate synthase (MtIPMS), Acetyl Coenzyme A
α-isopropylmalate synthase (MtIPMS)	Mycobacterium tuberculosis	acetyl-CoA	2.72	0.024	110 calc.	pH 7.5, 25 °C	PMID 19166329	Open record: α-isopropylmalate synthase (MtIPMS), acetyl-CoA
α-isopropylmalate synthase (MtIPMS)	Mycobacterium tuberculosis	α-ketoisovalerateTable 4 monovalent-cation block: Me+ = K+ (saturating)	3.2	0.016	210	pH 7.4, 25 °C	PMID 16684501	Open record: α-isopropylmalate synthase (MtIPMS), α-ketoisovalerate
α-isopropylmalate synthase (MtIPMS)	Mycobacterium tuberculosis	α-ketoisovalerateTable 4 monovalent-cation block: Me+ = Rb+ (saturating)	2.9	0.019	150	pH 7.4, 25 °C	PMID 16684501	Open record: α-isopropylmalate synthase (MtIPMS), α-ketoisovalerate
α-isopropylmalate synthase (MtIPMS)	Mycobacterium tuberculosis	α-ketoisovalerateTable 4 divalent-metal block: Me2+ = Mg2+ (saturating)	3.5	0.012	3.4 × 10³	pH 7.4, 25 °C	PMID 16684501	Open record: α-isopropylmalate synthase (MtIPMS), α-ketoisovalerate
α-isopropylmalate synthase (MtIPMS)	Mycobacterium tuberculosis	α-ketoisovalerateTable 4 divalent-metal block: Me2+ = Mn2+ (saturating)	1.69	0.0017	720	pH 7.4, 25 °C	PMID 16684501	Open record: α-isopropylmalate synthase (MtIPMS), α-ketoisovalerate
α-isopropylmalate synthase (MtIPMS)	Mycobacterium tuberculosis	α-ketoisovalerateTable 4 divalent-metal block: Me2+ = Co2+ (saturating)	0.77	0.0022	350	pH 7.4, 25 °C	PMID 16684501	Open record: α-isopropylmalate synthase (MtIPMS), α-ketoisovalerate
α-isopropylmalate synthase (MtIPMS)	Mycobacterium tuberculosis	α-ketoisovalerate	2.53	0.0071	360 calc.	pH 7.5, 25 °C	PMID 19166329	Open record: α-isopropylmalate synthase (MtIPMS), α-ketoisovalerate
α-isopropylmalate synthase (MtIPMS)variant E218A	Mycobacterium tuberculosis	acetyl-CoAphase 1 (unactivated form)	0.058	0.0095	6.1 calc.	pH 7.5, 25 °C	PMID 19166329	Open record: α-isopropylmalate synthase (MtIPMS) variant E218A, acetyl-CoA
α-isopropylmalate synthase (MtIPMS)variant E218A	Mycobacterium tuberculosis	acetyl-CoAphase 2 (activated form)	0.093	0.697	0.13 calc.	pH 7.5, 25 °C	PMID 19166329	Open record: α-isopropylmalate synthase (MtIPMS) variant E218A, acetyl-CoA
α-isopropylmalate synthase (MtIPMS)variant E218A	Mycobacterium tuberculosis	α-ketoisovaleratephase 1 (unactivated form)	0.037	0.0016	23 calc.	pH 7.5, 25 °C	PMID 19166329	Open record: α-isopropylmalate synthase (MtIPMS) variant E218A, α-ketoisovalerate
α-isopropylmalate synthase (MtIPMS)variant E218A	Mycobacterium tuberculosis	α-ketoisovaleratephase 2 (activated form)	0.022	0.039	0.56 calc.	pH 7.5, 25 °C	PMID 19166329	Open record: α-isopropylmalate synthase (MtIPMS) variant E218A, α-ketoisovalerate
α-isopropylmalate synthase (MtIPMS)variant H379A	Mycobacterium tuberculosis	acetyl-CoA	2.55	0.023	110 calc.	pH 7.5, 25 °C	PMID 19166329	Open record: α-isopropylmalate synthase (MtIPMS) variant H379A, acetyl-CoA
α-isopropylmalate synthase (MtIPMS)variant H379A	Mycobacterium tuberculosis	α-ketoisovaleratephase 1 (unactivated form)	0.7	0.0016	440 calc.	pH 7.5, 25 °C	PMID 19166329	Open record: α-isopropylmalate synthase (MtIPMS) variant H379A, α-ketoisovalerate
α-isopropylmalate synthase (MtIPMS)variant H379A	Mycobacterium tuberculosis	α-ketoisovaleratephase 2 (activated form)	1.48	0.019	78 calc.	pH 7.5, 25 °C	PMID 19166329	Open record: α-isopropylmalate synthase (MtIPMS) variant H379A, α-ketoisovalerate
α-isopropylmalate synthase (MtIPMS)variant Y169F	Mycobacterium tuberculosis	acetyl-CoA	5.67	0.04	140 calc.	pH 7.5, 25 °C	PMID 19166329	Open record: α-isopropylmalate synthase (MtIPMS) variant Y169F, acetyl-CoA
α-isopropylmalate synthase (MtIPMS)variant Y169F	Mycobacterium tuberculosis	α-ketoisovalerate	4.75	0.0134	354 calc.	pH 7.5, 25 °C	PMID 19166329	Open record: α-isopropylmalate synthase (MtIPMS) variant Y169F, α-ketoisovalerate
α-isopropylmalate synthase (MtIPMS)variant Y410F	Mycobacterium tuberculosis	acetyl-CoA	0.093	0.0011	85 calc.	pH 7.5, 25 °C	PMID 19166329	Open record: α-isopropylmalate synthase (MtIPMS) variant Y410F, acetyl-CoA
α-isopropylmalate synthase (MtIPMS)variant Y410F	Mycobacterium tuberculosis	α-ketoisovalerate	0.075	5 × 10⁻⁴	150 calc.	pH 7.5, 25 °C	PMID 19166329	Open record: α-isopropylmalate synthase (MtIPMS) variant Y410F, α-ketoisovalerate
α-isopropylmalate synthase (MtuIPMS)	Mycobacterium tuberculosis	acetyl coenzyme A	—	0.14	27	pH 7.5, 25 °C	PMID 23500460	Open record: α-isopropylmalate synthase (MtuIPMS), acetyl coenzyme A
α-isopropylmalate synthase (MtuIPMS)	Mycobacterium tuberculosis	α-ketoisovalerate	3.7	0.015	250	pH 7.5, 25 °C	PMID 23500460	Open record: α-isopropylmalate synthase (MtuIPMS), α-ketoisovalerate
α-isopropylmalate synthase (MtuIPMS)variant D444A	Mycobacterium tuberculosis	acetyl coenzyme A	—	0.042	70	pH 7.5, 25 °C	PMID 23500460	Open record: α-isopropylmalate synthase (MtuIPMS) variant D444A, acetyl coenzyme A
α-isopropylmalate synthase (MtuIPMS)variant D444A	Mycobacterium tuberculosis	α-ketoisovalerate	3.1	0.005	600	pH 7.5, 25 °C	PMID 23500460	Open record: α-isopropylmalate synthase (MtuIPMS) variant D444A, α-ketoisovalerate
α-isopropylmalate synthase (MtuIPMS)variant D444R	Mycobacterium tuberculosis	acetyl coenzyme A	—	0.027	210	pH 7.5, 25 °C	PMID 23500460	Open record: α-isopropylmalate synthase (MtuIPMS) variant D444R, acetyl coenzyme A
α-isopropylmalate synthase (MtuIPMS)variant D444R	Mycobacterium tuberculosis	α-ketoisovalerate	5.7	0.009	630	pH 7.5, 25 °C	PMID 23500460	Open record: α-isopropylmalate synthase (MtuIPMS) variant D444R, α-ketoisovalerate
α-isopropylmalate synthase (MtuIPMS)variant D444Y	Mycobacterium tuberculosis	acetyl coenzyme A	—	0.042	80	pH 7.5, 25 °C	PMID 23500460	Open record: α-isopropylmalate synthase (MtuIPMS) variant D444Y, acetyl coenzyme A
α-isopropylmalate synthase (MtuIPMS)variant D444Y	Mycobacterium tuberculosis	α-ketoisovalerate	3.4	0.016	210	pH 7.5, 25 °C	PMID 23500460	Open record: α-isopropylmalate synthase (MtuIPMS) variant D444Y, α-ketoisovalerate
α-isopropylmalate synthase (MtuIPMS)variant R97A	Mycobacterium tuberculosis	acetyl coenzyme A	—	0.21	33	pH 7.5, 25 °C	PMID 23500460	Open record: α-isopropylmalate synthase (MtuIPMS) variant R97A, acetyl coenzyme A
α-isopropylmalate synthase (MtuIPMS)variant R97A	Mycobacterium tuberculosis	α-ketoisovalerate	7	0.008	900	pH 7.5, 25 °C	PMID 23500460	Open record: α-isopropylmalate synthase (MtuIPMS) variant R97A, α-ketoisovalerate
α-isopropylmalate synthase (NmeIPMS)	Neisseria meningitidis	2-oxobutanoate	3.3	1.36	2.4 calc.	pH 7.5, 25 °C	PMID 20117081	Open record: α-isopropylmalate synthase (NmeIPMS), 2-oxobutanoate
α-isopropylmalate synthase (NmeIPMS)	Neisseria meningitidis	2-oxopentanoate (α-ketovalerate)	3.4	0.62	5.5 calc.	pH 7.5, 25 °C	PMID 20117081	Open record: α-isopropylmalate synthase (NmeIPMS), 2-oxopentanoate (α-ketovalerate)
α-isopropylmalate synthase (NmeIPMS)	Neisseria meningitidis	3-fluoropyruvate	0.3	105	0.0029 calc.	pH 7.5, 25 °C	PMID 20117081	Open record: α-isopropylmalate synthase (NmeIPMS), 3-fluoropyruvate
α-isopropylmalate synthase (NmeIPMS)	Neisseria meningitidis	3-methyl-2-oxopentanoate (α-keto-β-methylvalerate)	0.027	0.28	0.096 calc.	pH 7.5, 25 °C	PMID 20117081	Open record: α-isopropylmalate synthase (NmeIPMS), 3-methyl-2-oxopentanoate (α-keto-β-methylvalerate)
α-isopropylmalate synthase (NmeIPMS)	Neisseria meningitidis	acetyl coenzyme Afreshly purified enzyme; cosubstrate α-KIV	—	0.035	—	pH 7.5, 25 °C	PMID 20117081	Open record: α-isopropylmalate synthase (NmeIPMS), acetyl coenzyme A
α-isopropylmalate synthase (NmeIPMS)	Neisseria meningitidis	acetyl coenzyme Auncoupled AcCoA hydrolysis; no α-KIV; 3.3 μM enzyme	0.011	0.25	0.044 calc.	pH 7.5, 25 °C	PMID 20117081	Open record: α-isopropylmalate synthase (NmeIPMS), acetyl coenzyme A
α-isopropylmalate synthase (NmeIPMS)	Neisseria meningitidis	pyruvate	0.63	1.5	0.42 calc.	pH 7.5, 25 °C	PMID 20117081	Open record: α-isopropylmalate synthase (NmeIPMS), pyruvate
α-isopropylmalate synthase (NmeIPMS)	Neisseria meningitidis	α-ketoisovalerate20 mM KCl; freshly purified enzyme	13	0.03	430 calc.	pH 7.5, 25 °C	PMID 20117081	Open record: α-isopropylmalate synthase (NmeIPMS), α-ketoisovalerate
α-isopropylmalate synthase (NmeIPMS)	Neisseria meningitidis	α-ketoisovaleratedialysed enzyme; 0.6 mM KCl; 80 μM AcCoA	5.4	0.035	150 calc.	pH 7.5, 25 °C	PMID 20117081	Open record: α-isopropylmalate synthase (NmeIPMS), α-ketoisovalerate
α-isopropylmalate synthase (NmeIPMS)variant E365Term	Neisseria meningitidis	acetyl coenzyme A	0.01	0.15	0.067 calc.	pH 7.5, 25 °C	PMID 20117081	Open record: α-isopropylmalate synthase (NmeIPMS) variant E365Term, acetyl coenzyme A