Values CatLog read from this paper
Amino-acid substitutions at the domain interface affect substrate and allosteric inhibitor binding in α-isopropylmalate synthase from Mycobacterium tuberculosis
PMID 23500460doi:10.1016/j.bbrc.2013.02.092Open in Browse
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CatLog read 10 records (25 values) from this paper: α-isopropylmalate synthase (MtuIPMS) from Mycobacterium tuberculosis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| α-isopropylmalate synthase (MtuIPMS) | Mycobacterium tuberculosis | acetyl coenzyme A | — | 0.14 | 27 | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtuIPMS), acetyl coenzyme A |
| α-isopropylmalate synthase (MtuIPMS) | Mycobacterium tuberculosis | α-ketoisovalerate | 3.7 | 0.015 | 250 | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtuIPMS), α-ketoisovalerate |
| α-isopropylmalate synthase (MtuIPMS)variant D444A | Mycobacterium tuberculosis | acetyl coenzyme A | — | 0.042 | 70 | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtuIPMS) variant D444A, acetyl coenzyme A |
| α-isopropylmalate synthase (MtuIPMS)variant D444A | Mycobacterium tuberculosis | α-ketoisovalerate | 3.1 | 0.005 | 600 | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtuIPMS) variant D444A, α-ketoisovalerate |
| α-isopropylmalate synthase (MtuIPMS)variant D444R | Mycobacterium tuberculosis | acetyl coenzyme A | — | 0.027 | 210 | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtuIPMS) variant D444R, acetyl coenzyme A |
| α-isopropylmalate synthase (MtuIPMS)variant D444R | Mycobacterium tuberculosis | α-ketoisovalerate | 5.7 | 0.009 | 630 | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtuIPMS) variant D444R, α-ketoisovalerate |
| α-isopropylmalate synthase (MtuIPMS)variant D444Y | Mycobacterium tuberculosis | acetyl coenzyme A | — | 0.042 | 80 | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtuIPMS) variant D444Y, acetyl coenzyme A |
| α-isopropylmalate synthase (MtuIPMS)variant D444Y | Mycobacterium tuberculosis | α-ketoisovalerate | 3.4 | 0.016 | 210 | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtuIPMS) variant D444Y, α-ketoisovalerate |
| α-isopropylmalate synthase (MtuIPMS)variant R97A | Mycobacterium tuberculosis | acetyl coenzyme A | — | 0.21 | 33 | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtuIPMS) variant R97A, acetyl coenzyme A |
| α-isopropylmalate synthase (MtuIPMS)variant R97A | Mycobacterium tuberculosis | α-ketoisovalerate | 7 | 0.008 | 900 | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtuIPMS) variant R97A, α-ketoisovalerate |