Values CatLog read from this paper
The C-terminal regulatory domain is required for catalysis by Neisseria meningitidis alpha-isopropylmalate synthase
PMID 20117081doi:10.1016/j.bbrc.2010.01.114Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (19 values) from this paper: α-isopropylmalate synthase (NmeIPMS) from Neisseria meningitidis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| α-isopropylmalate synthase (NmeIPMS) | Neisseria meningitidis | 2-oxobutanoate | 3.3 | 1.36 | 2.4 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (NmeIPMS), 2-oxobutanoate |
| α-isopropylmalate synthase (NmeIPMS) | Neisseria meningitidis | 2-oxopentanoate (α-ketovalerate) | 3.4 | 0.62 | 5.5 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (NmeIPMS), 2-oxopentanoate (α-ketovalerate) |
| α-isopropylmalate synthase (NmeIPMS) | Neisseria meningitidis | 3-fluoropyruvate | 0.3 | 105 | 0.0029 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (NmeIPMS), 3-fluoropyruvate |
| α-isopropylmalate synthase (NmeIPMS) | Neisseria meningitidis | 3-methyl-2-oxopentanoate (α-keto-β-methylvalerate) | 0.027 | 0.28 | 0.096 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (NmeIPMS), 3-methyl-2-oxopentanoate (α-keto-β-methylvalerate) |
| α-isopropylmalate synthase (NmeIPMS) | Neisseria meningitidis | acetyl coenzyme Afreshly purified enzyme; cosubstrate α-KIV | — | 0.035 | — | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (NmeIPMS), acetyl coenzyme A |
| α-isopropylmalate synthase (NmeIPMS) | Neisseria meningitidis | acetyl coenzyme Auncoupled AcCoA hydrolysis; no α-KIV; 3.3 μM enzyme | 0.011 | 0.25 | 0.044 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (NmeIPMS), acetyl coenzyme A |
| α-isopropylmalate synthase (NmeIPMS) | Neisseria meningitidis | pyruvate | 0.63 | 1.5 | 0.42 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (NmeIPMS), pyruvate |
| α-isopropylmalate synthase (NmeIPMS) | Neisseria meningitidis | α-ketoisovalerate20 mM KCl; freshly purified enzyme | 13 | 0.03 | 430 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (NmeIPMS), α-ketoisovalerate |
| α-isopropylmalate synthase (NmeIPMS) | Neisseria meningitidis | α-ketoisovaleratedialysed enzyme; 0.6 mM KCl; 80 μM AcCoA | 5.4 | 0.035 | 150 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (NmeIPMS), α-ketoisovalerate |
| α-isopropylmalate synthase (NmeIPMS)variant E365Term | Neisseria meningitidis | acetyl coenzyme A | 0.01 | 0.15 | 0.067 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (NmeIPMS) variant E365Term, acetyl coenzyme A |