Values CatLog read from this paper
Amenability of the Gatekeeper Enzyme HphA to Engineering in the Homologation Pathway of l‑Phenylalanine and l‑Tyrosine through Homology-Based Site-Directed Mutagenesis
PMID 41799147doi:10.1021/acsomega.5c12112Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (30 values) from this paper: HphA (2-benzylmalate synthase), HCS (homocitrate synthase), IPMS (2-isopropylmalate synthase) from Nostoc punctiforme.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| HCS (homocitrate synthase) | — | oxaloacetic acid | — | — | 0.006 | pH 7.5, 20.6 °C | Open record: HCS (homocitrate synthase), oxaloacetic acid |
| HCS (homocitrate synthase) | — | α-ketoglutaric acid | 0.065 | 0.92 | 0.07 | pH 7.5, 20.6 °C | Open record: HCS (homocitrate synthase), α-ketoglutaric acid |
| HphA (2-benzylmalate synthase) | Nostoc punctiforme | 3-methyl-2-oxobutanoic acid | 0.0078 | 4 | 0.0019 | pH 7.5, 20.6 °C | Open record: HphA (2-benzylmalate synthase), 3-methyl-2-oxobutanoic acid |
| HphA (2-benzylmalate synthase) | Nostoc punctiforme | 4-methyl-2-oxovaleric acid | — | — | 0.0034 | pH 7.5, 20.6 °C | Open record: HphA (2-benzylmalate synthase), 4-methyl-2-oxovaleric acid |
| HphA (2-benzylmalate synthase)variant A73L | Nostoc punctiforme | 3-methyl-2-oxobutanoic acid | 0.12 | 0.65 | 0.18 | pH 7.5, 20.6 °C | Open record: HphA (2-benzylmalate synthase) variant A73L, 3-methyl-2-oxobutanoic acid |
| HphA (2-benzylmalate synthase)variant A73L | Nostoc punctiforme | 4-hydroxyphenylpyruvic acid | 0.22 | 0.41 | 0.54 | pH 7.5, 20.6 °C | Open record: HphA (2-benzylmalate synthase) variant A73L, 4-hydroxyphenylpyruvic acid |
| HphA (2-benzylmalate synthase)variant A73L | Nostoc punctiforme | 4-methyl-2-oxovaleric acid | 0.1 | 1.6 | 0.064 | pH 7.5, 20.6 °C | Open record: HphA (2-benzylmalate synthase) variant A73L, 4-methyl-2-oxovaleric acid |
| HphA (2-benzylmalate synthase)variant A73L | Nostoc punctiforme | hydroxypyruvic acid | 0.0046 | 3.2 | 0.0014 | pH 7.5, 20.6 °C | Open record: HphA (2-benzylmalate synthase) variant A73L, hydroxypyruvic acid |
| HphA (2-benzylmalate synthase)variant A73L | Nostoc punctiforme | indole-3-pyruvic acid | 0.0091 | 0.21 | 0.043 | pH 7.5, 20.6 °C | Open record: HphA (2-benzylmalate synthase) variant A73L, indole-3-pyruvic acid |
| HphA (2-benzylmalate synthase)variant A73L | Nostoc punctiforme | oxaloacetic acid | 0.0069 | — | — | pH 7.5, 20.6 °C | Open record: HphA (2-benzylmalate synthase) variant A73L, oxaloacetic acid |
| IPMS (2-isopropylmalate synthase) | — | 3-methyl-2-oxobutanoic acid | 0.7 | 0.11 | 6.4 | pH 7.5, 20.6 °C | Open record: IPMS (2-isopropylmalate synthase), 3-methyl-2-oxobutanoic acid |
| IPMS (2-isopropylmalate synthase) | — | 4-methyl-2-oxovaleric acid | 0.013 | 0.011 | 1.1 | pH 7.5, 20.6 °C | Open record: IPMS (2-isopropylmalate synthase), 4-methyl-2-oxovaleric acid |