Values CatLog read from this paper
Kinetic evidence for interdomain communication in the allosteric regulation of alpha-isopropylmalate synthase from Mycobacterium tuberculosis
PMID 19166329doi:10.1021/bi801707tOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (26 values) from this paper: α-isopropylmalate synthase (MtIPMS) from Mycobacterium tuberculosis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 13 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| α-isopropylmalate synthase (MtIPMS) | Mycobacterium tuberculosis | acetyl-CoA | 2.72 | 0.024 | 110 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtIPMS), acetyl-CoA |
| α-isopropylmalate synthase (MtIPMS) | Mycobacterium tuberculosis | α-ketoisovalerate | 2.53 | 0.0071 | 360 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtIPMS), α-ketoisovalerate |
| α-isopropylmalate synthase (MtIPMS)variant E218A | Mycobacterium tuberculosis | acetyl-CoAphase 1 (unactivated form) | 0.058 | 0.0095 | 6.1 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtIPMS) variant E218A, acetyl-CoA |
| α-isopropylmalate synthase (MtIPMS)variant E218A | Mycobacterium tuberculosis | acetyl-CoAphase 2 (activated form) | 0.093 | 0.697 | 0.13 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtIPMS) variant E218A, acetyl-CoA |
| α-isopropylmalate synthase (MtIPMS)variant E218A | Mycobacterium tuberculosis | α-ketoisovaleratephase 1 (unactivated form) | 0.037 | 0.0016 | 23 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtIPMS) variant E218A, α-ketoisovalerate |
| α-isopropylmalate synthase (MtIPMS)variant E218A | Mycobacterium tuberculosis | α-ketoisovaleratephase 2 (activated form) | 0.022 | 0.039 | 0.56 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtIPMS) variant E218A, α-ketoisovalerate |
| α-isopropylmalate synthase (MtIPMS)variant H379A | Mycobacterium tuberculosis | acetyl-CoA | 2.55 | 0.023 | 110 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtIPMS) variant H379A, acetyl-CoA |
| α-isopropylmalate synthase (MtIPMS)variant H379A | Mycobacterium tuberculosis | α-ketoisovaleratephase 1 (unactivated form) | 0.7 | 0.0016 | 440 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtIPMS) variant H379A, α-ketoisovalerate |
| α-isopropylmalate synthase (MtIPMS)variant H379A | Mycobacterium tuberculosis | α-ketoisovaleratephase 2 (activated form) | 1.48 | 0.019 | 78 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtIPMS) variant H379A, α-ketoisovalerate |
| α-isopropylmalate synthase (MtIPMS)variant Y169F | Mycobacterium tuberculosis | acetyl-CoA | 5.67 | 0.04 | 140 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtIPMS) variant Y169F, acetyl-CoA |
| α-isopropylmalate synthase (MtIPMS)variant Y169F | Mycobacterium tuberculosis | α-ketoisovalerate | 4.75 | 0.0134 | 354 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtIPMS) variant Y169F, α-ketoisovalerate |
| α-isopropylmalate synthase (MtIPMS)variant Y410F | Mycobacterium tuberculosis | acetyl-CoA | 0.093 | 0.0011 | 85 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtIPMS) variant Y410F, acetyl-CoA |
| α-isopropylmalate synthase (MtIPMS)variant Y410F | Mycobacterium tuberculosis | α-ketoisovalerate | 0.075 | 5 × 10⁻⁴ | 150 calc. | pH 7.5, 25 °C | Open record: α-isopropylmalate synthase (MtIPMS) variant Y410F, α-ketoisovalerate |