Values CatLog read from this paper

Critical roles of conserved carboxylic acid residues in pigeon cytosolic NADP+-dependent malic enzyme

PMID 16889632doi:10.1111/j.1742-4658.2006.05409.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 23 records (41 values) from this paper: cytosolic NADP+-dependent malic enzyme from pigeon.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 18 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.40

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
cytosolic NADP+-dependent malic enzyme	pigeon	L-malatemetal Mn2+	31.34	0.08	390 calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme, L-malate
cytosolic NADP+-dependent malic enzyme	pigeon	L-malatemetal Mg2+ (replaces Mn2+)	34.83	0.27	130 calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme, L-malate
cytosolic NADP+-dependent malic enzyme	pigeon	L-malatewith 50 mM sodium azide; metal Mn2+	27.8	0.18	150 calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme, L-malate
cytosolic NADP+-dependent malic enzyme	pigeon	nicotinamide adenine dinucleotide phosphate (oxidized)	—	0.00207	—	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme, nicotinamide adenine dinucleotide phosphate (oxidized)
cytosolic NADP+-dependent malic enzyme	pigeon	oxaloacetate	33.41	0.17	200 calc.	pH 4.5	Open record: cytosolic NADP+-dependent malic enzyme, oxaloacetate
cytosolic NADP+-dependent malic enzyme	pigeon	pyruvate1-50 mM pyruvate	0.8	6.05	0.13 calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme, pyruvate
cytosolic NADP+-dependent malic enzyme	pigeon	pyruvatewith 50 mM sodium azide	0.87	0.65	1.3 calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme, pyruvate
cytosolic NADP+-dependent malic enzymevariant D235A	pigeon	L-malate	0.004	0.1	0.040 calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme variant D235A, L-malate
cytosolic NADP+-dependent malic enzymevariant D235A	pigeon	nicotinamide adenine dinucleotide phosphate (oxidized)	—	0.00179	—	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme variant D235A, nicotinamide adenine dinucleotide phosphate (oxidized)
cytosolic NADP+-dependent malic enzymevariant D235A	pigeon	oxaloacetate	0.09	0.91	0.099 calc.	pH 4.5	Open record: cytosolic NADP+-dependent malic enzyme variant D235A, oxaloacetate
cytosolic NADP+-dependent malic enzymevariant D235A	pigeon	pyruvate	0.001	6.11	1.6 × 10⁻⁴ calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme variant D235A, pyruvate
cytosolic NADP+-dependent malic enzymevariant D257A	pigeon	L-malate	1.1	0.1	11 calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme variant D257A, L-malate
cytosolic NADP+-dependent malic enzymevariant D257A	pigeon	nicotinamide adenine dinucleotide phosphate (oxidized)	—	0.00283	—	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme variant D257A, nicotinamide adenine dinucleotide phosphate (oxidized)
cytosolic NADP+-dependent malic enzymevariant D257A	pigeon	oxaloacetate	76.72	0.07	1.1 × 10³ calc.	pH 4.5	Open record: cytosolic NADP+-dependent malic enzyme variant D257A, oxaloacetate
cytosolic NADP+-dependent malic enzymevariant D257A	pigeon	pyruvate	0.18	1.42	0.13 calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme variant D257A, pyruvate
cytosolic NADP+-dependent malic enzymevariant D258A	pigeon	L-malate	0.006	0.05	0.12 calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme variant D258A, L-malate
cytosolic NADP+-dependent malic enzymevariant D258A	pigeon	L-malatewith 50 mM sodium azide	11.76	3.2	3.7 calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme variant D258A, L-malate
cytosolic NADP+-dependent malic enzymevariant D258A	pigeon	nicotinamide adenine dinucleotide phosphate (oxidized)	—	0.00296	—	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme variant D258A, nicotinamide adenine dinucleotide phosphate (oxidized)
cytosolic NADP+-dependent malic enzymevariant D258A	pigeon	oxaloacetate	0.22	2.37	0.093 calc.	pH 4.5	Open record: cytosolic NADP+-dependent malic enzyme variant D258A, oxaloacetate
cytosolic NADP+-dependent malic enzymevariant D258A	pigeon	pyruvate1-50 mM pyruvate	0.0014	1.9	7.4 × 10⁻⁴ calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme variant D258A, pyruvate
cytosolic NADP+-dependent malic enzymevariant D258A	pigeon	pyruvatewith 50 mM sodium azide	0.84	5.69	0.15 calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme variant D258A, pyruvate
cytosolic NADP+-dependent malic enzymevariant E234A	pigeon	L-malate	46.44	13.33	3.484 calc.	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme variant E234A, L-malate
cytosolic NADP+-dependent malic enzymevariant E234A	pigeon	nicotinamide adenine dinucleotide phosphate (oxidized)	—	0.0018	—	pH 7.4, 25 °C	Open record: cytosolic NADP+-dependent malic enzyme variant E234A, nicotinamide adenine dinucleotide phosphate (oxidized)