Values CatLog read from this paper
Characterization of the acyl substrate binding pocket of acetyl-CoA synthetase
PMID 16981708doi:10.1021/bi061023eOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 35 records (78 values) from this paper: acetyl-CoA synthetase (MT-ACS1) from Methanothermobacter thermautotrophicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| acetyl-CoA synthetase (MT-ACS1) | Methanothermobacter thermautotrophicus | acetate | 65.4 | 3.5 | 18.6 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1), acetate |
| acetyl-CoA synthetase (MT-ACS1) | Methanothermobacter thermautotrophicus | coenzyme A | — | 0.19 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1), coenzyme A |
| acetyl-CoA synthetase (MT-ACS1) | Methanothermobacter thermautotrophicus | Mg2+-ATP | — | 3.3 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1), Mg2+-ATP |
| acetyl-CoA synthetase (MT-ACS1) | Methanothermobacter thermautotrophicus | propionate | 46.3 | 36.5 | 1.3 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1), propionate |
| acetyl-CoA synthetase (MT-ACS1)variant I312A | Methanothermobacter thermautotrophicus | acetate | 30.4 | 24.6 | 1.24 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant I312A, acetate |
| acetyl-CoA synthetase (MT-ACS1)variant I312A | Methanothermobacter thermautotrophicus | butyrate | 0.47 | 136.4 | 0.0034 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant I312A, butyrate |
| acetyl-CoA synthetase (MT-ACS1)variant I312A | Methanothermobacter thermautotrophicus | coenzyme A | — | 0.18 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant I312A, coenzyme A |
| acetyl-CoA synthetase (MT-ACS1)variant I312A | Methanothermobacter thermautotrophicus | Mg2+-ATP | — | 6.6 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant I312A, Mg2+-ATP |
| acetyl-CoA synthetase (MT-ACS1)variant I312A | Methanothermobacter thermautotrophicus | propionate | 18.2 | 73.1 | 0.25 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant I312A, propionate |
| acetyl-CoA synthetase (MT-ACS1)variant T313K | Methanothermobacter thermautotrophicus | acetate | 1.9 | 596 | 0.0032 calc. | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant T313K, acetate |
| acetyl-CoA synthetase (MT-ACS1)variant T313K | Methanothermobacter thermautotrophicus | Mg2+-ATP | — | 3.4 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant T313K, Mg2+-ATP |
| acetyl-CoA synthetase (MT-ACS1)variant T313V | Methanothermobacter thermautotrophicus | acetate | 3.4 | 0.46 | 7.3 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant T313V, acetate |
| acetyl-CoA synthetase (MT-ACS1)variant T313V | Methanothermobacter thermautotrophicus | coenzyme A | — | 0.7 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant T313V, coenzyme A |
| acetyl-CoA synthetase (MT-ACS1)variant T313V | Methanothermobacter thermautotrophicus | Mg2+-ATP | — | 0.65 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant T313V, Mg2+-ATP |
| acetyl-CoA synthetase (MT-ACS1)variant T313V | Methanothermobacter thermautotrophicus | propionate | 5.9 | 4.5 | 1.31 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant T313V, propionate |
| acetyl-CoA synthetase (MT-ACS1)variant V388A | Methanothermobacter thermautotrophicus | acetate | 36.2 | 13.2 | 2.7 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant V388A, acetate |
| acetyl-CoA synthetase (MT-ACS1)variant V388A | Methanothermobacter thermautotrophicus | butyrate | 1.56 | 151.9 | 0.01 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant V388A, butyrate |
| acetyl-CoA synthetase (MT-ACS1)variant V388A | Methanothermobacter thermautotrophicus | coenzyme A | — | 0.35 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant V388A, coenzyme A |
| acetyl-CoA synthetase (MT-ACS1)variant V388A | Methanothermobacter thermautotrophicus | Mg2+-ATP | — | 2.6 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant V388A, Mg2+-ATP |
| acetyl-CoA synthetase (MT-ACS1)variant V388A | Methanothermobacter thermautotrophicus | propionate | 13.2 | 4.1 | 3.2 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant V388A, propionate |
| acetyl-CoA synthetase (MT-ACS1)variant V388G | Methanothermobacter thermautotrophicus | acetate | 31.9 | 164.4 | 0.2 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant V388G, acetate |
| acetyl-CoA synthetase (MT-ACS1)variant V388G | Methanothermobacter thermautotrophicus | coenzyme A | — | 0.18 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant V388G, coenzyme A |
| acetyl-CoA synthetase (MT-ACS1)variant V388G | Methanothermobacter thermautotrophicus | Mg2+-ATP | — | 4 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant V388G, Mg2+-ATP |
| acetyl-CoA synthetase (MT-ACS1)variant V388G | Methanothermobacter thermautotrophicus | propionate | 6 | 128.6 | 0.05 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant V388G, propionate |
| acetyl-CoA synthetase (MT-ACS1)variant W416G | Methanothermobacter thermautotrophicus | 3-methylvalerate | 0.67 | 29.2 | 0.023 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant W416G, 3-methylvalerate |
| acetyl-CoA synthetase (MT-ACS1)variant W416G | Methanothermobacter thermautotrophicus | 4-methylvalerate | 10.9 | 7.6 | 1.42 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant W416G, 4-methylvalerate |
| acetyl-CoA synthetase (MT-ACS1)variant W416G | Methanothermobacter thermautotrophicus | acetate | 33.7 | 132.1 | 0.26 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant W416G, acetate |
| acetyl-CoA synthetase (MT-ACS1)variant W416G | Methanothermobacter thermautotrophicus | butyrate | 10.8 | 39.2 | 0.27 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant W416G, butyrate |
| acetyl-CoA synthetase (MT-ACS1)variant W416G | Methanothermobacter thermautotrophicus | coenzyme A | — | 0.43 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant W416G, coenzyme A |
| acetyl-CoA synthetase (MT-ACS1)variant W416G | Methanothermobacter thermautotrophicus | heptanoate | 7.3 | 10.2 | 0.72 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant W416G, heptanoate |
| acetyl-CoA synthetase (MT-ACS1)variant W416G | Methanothermobacter thermautotrophicus | hexanoate | 5.9 | 6.1 | 0.96 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant W416G, hexanoate |
| acetyl-CoA synthetase (MT-ACS1)variant W416G | Methanothermobacter thermautotrophicus | Mg2+-ATP | — | 2.3 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant W416G, Mg2+-ATP |
| acetyl-CoA synthetase (MT-ACS1)variant W416G | Methanothermobacter thermautotrophicus | octanoate | 3.3 | 18.1 | 0.18 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant W416G, octanoate |
| acetyl-CoA synthetase (MT-ACS1)variant W416G | Methanothermobacter thermautotrophicus | propionate | 19.2 | 188.8 | 0.1 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant W416G, propionate |
| acetyl-CoA synthetase (MT-ACS1)variant W416G | Methanothermobacter thermautotrophicus | valerate | 11.9 | 11.1 | 1.07 | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase (MT-ACS1) variant W416G, valerate |