Values CatLog read from this paper

Regulatory mechanisms differ in UMP kinases from gram-negative and gram-positive bacteria

PMID 17210578doi:10.1074/jbc.m606963200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 62 records (62 values) from this paper: UMP kinase from Escherichia coli, Bacillus subtilis, Neisseria meningitidis and 2 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.4.22

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
UMP kinase	Bacillus subtilis	uridine 5'-diphosphateno activator	—	0.004	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-diphosphate
UMP kinase	Bacillus subtilis	uridine 5'-diphosphate	—	0.0241	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-diphosphate
UMP kinase	Bacillus subtilis	uridine 5'-monophosphateUMP varied at fixed 2.0 mM ATP; no GTP	—	0.01	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Bacillus subtilis	uridine 5'-monophosphateUMP varied at fixed 2.0 mM ATP; 0.5 mM GTP	—	0.0274	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Bacillus subtilis	uridine 5'-monophosphateUMP varied at fixed 30.0 mM ATP; no GTP	—	0.131	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Bacillus subtilis	uridine 5'-monophosphateUMP varied at fixed 30.0 mM ATP; 0.5 mM GTP	—	0.155	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinasevariant N137A	Bacillus subtilis	adenosine 5'-triphosphateno GTP	—	10.5	—	pH 7.4, 30 °C	Open record: UMP kinase variant N137A, adenosine 5'-triphosphate
UMP kinasevariant N137A	Bacillus subtilis	adenosine 5'-triphosphate	—	1	—	pH 7.4, 30 °C	Open record: UMP kinase variant N137A, adenosine 5'-triphosphate
UMP kinasevariant N137A	Bacillus subtilis	uridine 5'-monophosphateno GTP	—	0.0127	—	pH 7.4, 30 °C	Open record: UMP kinase variant N137A, uridine 5'-monophosphate
UMP kinasevariant N137A	Bacillus subtilis	uridine 5'-monophosphate	—	0.0178	—	pH 7.4, 30 °C	Open record: UMP kinase variant N137A, uridine 5'-monophosphate
UMP kinasevariant T135A	Bacillus subtilis	adenosine 5'-triphosphateno GTP	—	9.5	—	pH 7.4, 30 °C	Open record: UMP kinase variant T135A, adenosine 5'-triphosphate
UMP kinasevariant T135A	Bacillus subtilis	adenosine 5'-triphosphate	—	1.2	—	pH 7.4, 30 °C	Open record: UMP kinase variant T135A, adenosine 5'-triphosphate
UMP kinasevariant T135A	Bacillus subtilis	uridine 5'-monophosphateno GTP	—	0.0475	—	pH 7.4, 30 °C	Open record: UMP kinase variant T135A, uridine 5'-monophosphate
UMP kinasevariant T135A	Bacillus subtilis	uridine 5'-monophosphate	—	0.0807	—	pH 7.4, 30 °C	Open record: UMP kinase variant T135A, uridine 5'-monophosphate
UMP kinasevariant T135A/N137A	Bacillus subtilis	adenosine 5'-triphosphateno GTP	—	10	—	pH 7.4, 30 °C	Open record: UMP kinase variant T135A/N137A, adenosine 5'-triphosphate
UMP kinasevariant T135A/N137A	Bacillus subtilis	adenosine 5'-triphosphate	—	2.2	—	pH 7.4, 30 °C	Open record: UMP kinase variant T135A/N137A, adenosine 5'-triphosphate
UMP kinasevariant T135A/N137A	Bacillus subtilis	uridine 5'-monophosphateno GTP	—	0.0531	—	pH 7.4, 30 °C	Open record: UMP kinase variant T135A/N137A, uridine 5'-monophosphate
UMP kinasevariant T135A/N137A	Bacillus subtilis	uridine 5'-monophosphate	—	0.143	—	pH 7.4, 30 °C	Open record: UMP kinase variant T135A/N137A, uridine 5'-monophosphate
UMP kinasevariant D159N	Escherichia coli	adenosine 5'-triphosphateATP varied at fixed 0.1 mM UMP; no GTP; Table 4	—	0.23	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, adenosine 5'-triphosphate
UMP kinasevariant D159N	Escherichia coli	adenosine 5'-triphosphateATP varied at fixed 0.1 mM UMP; 0.5 mM GTP; Table 4	—	0.27	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, adenosine 5'-triphosphate
UMP kinasevariant D159N	Escherichia coli	adenosine 5'-triphosphateATP varied at fixed 1.0 mM UMP; no GTP; Table 4	—	0.15	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, adenosine 5'-triphosphate
UMP kinasevariant D159N	Escherichia coli	adenosine 5'-triphosphateATP varied at fixed 1.0 mM UMP; 0.5 mM GTP; Table 4	—	0.2	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, adenosine 5'-triphosphate
UMP kinasevariant D159N	Escherichia coli	adenosine 5'-triphosphateATP varied at constant 0.3 mM UMP; effector: none; Table 6	—	0.21	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, adenosine 5'-triphosphate
UMP kinasevariant D159N	Escherichia coli	adenosine 5'-triphosphateATP varied at constant 0.3 mM UMP; effector: 0.5 mM GTP	—	0.24	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, adenosine 5'-triphosphate
UMP kinasevariant D159N	Escherichia coli	adenosine 5'-triphosphateATP varied at constant 0.3 mM UMP; apparent Km; Table 6	—	0.77	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, adenosine 5'-triphosphate
UMP kinasevariant D159N	Escherichia coli	adenosine 5'-triphosphateATP varied at constant 0.3 mM UMP; apparent Km; Table 6	—	0.26	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, adenosine 5'-triphosphate
UMP kinasevariant D159N	Escherichia coli	uridine 5'-monophosphateUMP varied at fixed 0.2 mM ATP; no GTP; Table 5	—	0.0492	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, uridine 5'-monophosphate
UMP kinasevariant D159N	Escherichia coli	uridine 5'-monophosphateUMP varied at fixed 0.2 mM ATP; 0.5 mM GTP; Table 5	—	0.047	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, uridine 5'-monophosphate
UMP kinasevariant D159N	Escherichia coli	uridine 5'-monophosphateUMP varied at fixed 2.0 mM ATP; no GTP; Table 5	—	0.046	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, uridine 5'-monophosphate
UMP kinasevariant D159N	Escherichia coli	uridine 5'-monophosphateUMP varied at fixed 2.0 mM ATP; 0.5 mM GTP; Table 5	—	0.051	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, uridine 5'-monophosphate
UMP kinasevariant D159N	Escherichia coli	uridine 5'-monophosphateUMP varied at constant 0.2 mM ATP; effector: none; Table 6	—	0.05	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, uridine 5'-monophosphate
UMP kinasevariant D159N	Escherichia coli	uridine 5'-monophosphateUMP varied at constant 0.2 mM ATP; effector: 0.5 mM GTP	—	0.0593	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, uridine 5'-monophosphate
UMP kinasevariant D159N	Escherichia coli	uridine 5'-monophosphateUMP varied at constant 0.2 mM ATP; apparent Km; Table 6	—	1.69	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, uridine 5'-monophosphate
UMP kinasevariant D159N	Escherichia coli	uridine 5'-monophosphateUMP varied at constant 0.2 mM ATP; apparent Km; Table 6	—	0.207	—	pH 7.4, 30 °C	Open record: UMP kinase variant D159N, uridine 5'-monophosphate
UMP kinasevariant N140A	Escherichia coli	adenosine 5'-triphosphateeffector: none	—	0.25	—	pH 7.4, 30 °C	Open record: UMP kinase variant N140A, adenosine 5'-triphosphate
UMP kinasevariant N140A	Escherichia coli	adenosine 5'-triphosphateeffector: 0.5 mM GTP	—	0.21	—	pH 7.4, 30 °C	Open record: UMP kinase variant N140A, adenosine 5'-triphosphate
UMP kinasevariant N140A	Escherichia coli	adenosine 5'-triphosphateeffector: 0.5 mM UTP (inhibitor)	—	0.53	—	pH 7.4, 30 °C	Open record: UMP kinase variant N140A, adenosine 5'-triphosphate
UMP kinasevariant N140A	Escherichia coli	adenosine 5'-triphosphateeffector: 0.5 mM GTP + 0.5 mM UTP	—	0.23	—	pH 7.4, 30 °C	Open record: UMP kinase variant N140A, adenosine 5'-triphosphate
UMP kinasevariant N140A	Escherichia coli	uridine 5'-monophosphateeffector: none	—	0.05	—	pH 7.4, 30 °C	Open record: UMP kinase variant N140A, uridine 5'-monophosphate
UMP kinasevariant N140A	Escherichia coli	uridine 5'-monophosphateeffector: 0.5 mM GTP	—	0.0739	—	pH 7.4, 30 °C	Open record: UMP kinase variant N140A, uridine 5'-monophosphate
UMP kinasevariant N140A	Escherichia coli	uridine 5'-monophosphateeffector: 0.5 mM UTP (inhibitor)	—	0.1812	—	pH 7.4, 30 °C	Open record: UMP kinase variant N140A, uridine 5'-monophosphate
UMP kinasevariant N140A	Escherichia coli	uridine 5'-monophosphateeffector: 0.5 mM GTP + 0.5 mM UTP	—	0.1086	—	pH 7.4, 30 °C	Open record: UMP kinase variant N140A, uridine 5'-monophosphate
UMP kinase	Haemophilus influenzae	uridine 5'-monophosphateUMP varied at fixed 1.0 mM ATP; no GTP	—	0.04	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Haemophilus influenzae	uridine 5'-monophosphateUMP varied at fixed 1.0 mM ATP; 0.5 mM GTP	—	0.04	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Haemophilus influenzae	uridine 5'-monophosphateUMP varied at fixed 12.0 mM ATP; no GTP	—	0.1	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Haemophilus influenzae	uridine 5'-monophosphateUMP varied at fixed 12.0 mM ATP; 0.5 mM GTP	—	0.05	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Neisseria meningitidis	adenosine 5'-triphosphateATP varied at fixed 0.05 mM UMP; no GTP	—	2.98	—	pH 7.4, 30 °C	Open record: UMP kinase, adenosine 5'-triphosphate
UMP kinase	Neisseria meningitidis	adenosine 5'-triphosphateATP varied at fixed 0.05 mM UMP; 0.5 mM GTP	—	0.83	—	pH 7.4, 30 °C	Open record: UMP kinase, adenosine 5'-triphosphate
UMP kinase	Neisseria meningitidis	adenosine 5'-triphosphateATP varied at fixed 1.0 mM UMP; no GTP	—	3.22	—	pH 7.4, 30 °C	Open record: UMP kinase, adenosine 5'-triphosphate
UMP kinase	Neisseria meningitidis	adenosine 5'-triphosphateATP varied at fixed 1.0 mM UMP; 0.5 mM GTP	—	1.98	—	pH 7.4, 30 °C	Open record: UMP kinase, adenosine 5'-triphosphate
UMP kinase	Neisseria meningitidis	adenosine 5'-triphosphateno GTP	—	4.7	—	pH 7.4, 30 °C	Open record: UMP kinase, adenosine 5'-triphosphate
UMP kinase	Neisseria meningitidis	adenosine 5'-triphosphate0.5 mM GTP	—	1.7	—	pH 7.4, 30 °C	Open record: UMP kinase, adenosine 5'-triphosphate
UMP kinase	Neisseria meningitidis	uridine 5'-diphosphateno activator	—	0.0034	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-diphosphate
UMP kinase	Neisseria meningitidis	uridine 5'-diphosphate	—	0.0085	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-diphosphate
UMP kinase	Neisseria meningitidis	uridine 5'-monophosphateUMP varied at fixed 2.0 mM ATP; no GTP	—	0.0087	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Neisseria meningitidis	uridine 5'-monophosphateUMP varied at fixed 2.0 mM ATP; 0.5 mM GTP	—	0.01	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Neisseria meningitidis	uridine 5'-monophosphateUMP varied at fixed 10.0 mM ATP; no GTP	—	0.0156	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Neisseria meningitidis	uridine 5'-monophosphateUMP varied at fixed 10.0 mM ATP; 0.5 mM GTP	—	0.0574	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Streptococcus pneumoniae	uridine 5'-monophosphateUMP varied at fixed 2.0 mM ATP; no GTP	—	0.0222	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Streptococcus pneumoniae	uridine 5'-monophosphateUMP varied at fixed 2.0 mM ATP; 0.5 mM GTP	—	0.0997	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Streptococcus pneumoniae	uridine 5'-monophosphateUMP varied at fixed 30.0 mM ATP; no GTP	—	0.15	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate
UMP kinase	Streptococcus pneumoniae	uridine 5'-monophosphateUMP varied at fixed 30.0 mM ATP; 0.5 mM GTP	—	0.1056	—	pH 7.4, 30 °C	Open record: UMP kinase, uridine 5'-monophosphate