CatLogenzyme kinetics catalog

EC 2.7.4.22: UMP kinase

TransferasesOpen in Browse

CatLog read 84 records for EC 2.7.4.22 from 4 papers, for 4 organisms. Names in these papers: UMP kinase, UMP kinase (UMPKeco).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 49 records for EC 2.7.4.22 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.4.22 in Browse.

Papers

PaperOrganismsRecords
Regulatory mechanisms differ in UMP kinases from gram-negative and gram-positive bacteriaBacillus subtilis, Neisseria meningitidis30
Mutational analysis of UMP kinase from Escherichia coliEscherichia coli28
Structural and functional characterization of Escherichia coli UMP kinase in complex with its allosteric regulator GTPEscherichia coli22
UMP kinase from Streptococcus pneumoniae: evidence for co-operative ATP binding and allosteric regulationStreptococcus pneumoniae4

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
UMP kinaseBacillus subtilisuridine 5'-diphosphateno activator—0.004—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseBacillus subtilisuridine 5'-diphosphate—0.0241—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseBacillus subtilisuridine 5'-monophosphateUMP varied at fixed 2.0 mM ATP; no GTP—0.01—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseBacillus subtilisuridine 5'-monophosphateUMP varied at fixed 2.0 mM ATP; 0.5 mM GTP—0.0274—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseBacillus subtilisuridine 5'-monophosphateUMP varied at fixed 30.0 mM ATP; no GTP—0.131—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseBacillus subtilisuridine 5'-monophosphateUMP varied at fixed 30.0 mM ATP; 0.5 mM GTP—0.155—pH 7.4, 30 °CPMID 17210578Open
UMP kinasevariant N137ABacillus subtilisadenosine 5'-triphosphateno GTP—10.5—pH 7.4, 30 °CPMID 17210578Open
UMP kinasevariant N137ABacillus subtilisadenosine 5'-triphosphate—1—pH 7.4, 30 °CPMID 17210578Open
UMP kinasevariant N137ABacillus subtilisuridine 5'-monophosphateno GTP—0.0127—pH 7.4, 30 °CPMID 17210578Open
UMP kinasevariant N137ABacillus subtilisuridine 5'-monophosphate—0.0178—pH 7.4, 30 °CPMID 17210578Open
UMP kinasevariant T135ABacillus subtilisadenosine 5'-triphosphateno GTP—9.5—pH 7.4, 30 °CPMID 17210578Open
UMP kinasevariant T135ABacillus subtilisadenosine 5'-triphosphate—1.2—pH 7.4, 30 °CPMID 17210578Open
UMP kinasevariant T135ABacillus subtilisuridine 5'-monophosphateno GTP—0.0475—pH 7.4, 30 °CPMID 17210578Open
UMP kinasevariant T135ABacillus subtilisuridine 5'-monophosphate—0.0807—pH 7.4, 30 °CPMID 17210578Open
UMP kinasevariant T135A/N137ABacillus subtilisadenosine 5'-triphosphateno GTP—10—pH 7.4, 30 °CPMID 17210578Open
UMP kinasevariant T135A/N137ABacillus subtilisadenosine 5'-triphosphate—2.2—pH 7.4, 30 °CPMID 17210578Open
UMP kinasevariant T135A/N137ABacillus subtilisuridine 5'-monophosphateno GTP—0.0531—pH 7.4, 30 °CPMID 17210578Open
UMP kinasevariant T135A/N137ABacillus subtilisuridine 5'-monophosphate—0.143—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseEscherichia coliadenosine 5'-triphosphate—0.048—pH 6, 30 °CPMID 9457846Open
UMP kinaseEscherichia coliuridine 5'-monophosphate—0.17—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant D146NEscherichia coliadenosine 5'-triphosphate—0.03—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant D146NEscherichia coliadenosine 5'-triphosphate—0.1—pH 8, 30 °CPMID 9457846Open
UMP kinasevariant D146NEscherichia coliuridine 5'-monophosphate—0.45—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant D146NEscherichia coliuridine 5'-monophosphate—0.059—pH 8, 30 °CPMID 9457846Open
UMP kinasevariant D159NEscherichia coliadenosine 5'-triphosphate—0.095—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant D159NEscherichia coliuridine 5'-monophosphate—0.27—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant D168NEscherichia coliadenosine 5'-triphosphate—0.14—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant D168NEscherichia coliadenosine 5'-triphosphate—0.42—pH 8, 30 °CPMID 9457846Open
UMP kinasevariant D168NEscherichia coliuridine 5'-monophosphate—0.32—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant D168NEscherichia coliuridine 5'-monophosphate—0.06—pH 8, 30 °CPMID 9457846Open
UMP kinasevariant D174NEscherichia coliadenosine 5'-triphosphate—0.17—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant D174NEscherichia coliadenosine 5'-triphosphate—0.31—pH 8, 30 °CPMID 9457846Open
UMP kinasevariant D174NEscherichia coliuridine 5'-monophosphate—3.7—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant D174NEscherichia coliuridine 5'-monophosphate—0.31—pH 8, 30 °CPMID 9457846Open
UMP kinasevariant D201NEscherichia coliadenosine 5'-triphosphate—0.2—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant D201NEscherichia coliadenosine 5'-triphosphate—0.46—pH 8, 30 °CPMID 9457846Open
UMP kinasevariant D201NEscherichia coliuridine 5'-monophosphate—0.86—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant D201NEscherichia coliuridine 5'-monophosphate—0.22—pH 8, 30 °CPMID 9457846Open
UMP kinasevariant D77NEscherichia coliadenosine 5'-triphosphate—0.12—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant D77NEscherichia coliadenosine 5'-triphosphate—0.48—pH 8, 30 °CPMID 9457846Open
UMP kinasevariant D77NEscherichia coliuridine 5'-monophosphate—1.78—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant D77NEscherichia coliuridine 5'-monophosphate—0.47—pH 8, 30 °CPMID 9457846Open
UMP kinasevariant R62HEscherichia coliadenosine 5'-triphosphate—0.24—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant R62HEscherichia coliadenosine 5'-triphosphate—3—pH 8, 30 °CPMID 9457846Open
UMP kinasevariant R62HEscherichia coliuridine 5'-monophosphate—1.26—pH 6, 30 °CPMID 9457846Open
UMP kinasevariant R62HEscherichia coliuridine 5'-monophosphate—0.32—pH 8, 30 °CPMID 9457846Open
UMP kinaseNeisseria meningitidisadenosine 5'-triphosphateATP varied at fixed 0.05 mM UMP; no GTP—2.98—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseNeisseria meningitidisadenosine 5'-triphosphateATP varied at fixed 0.05 mM UMP; 0.5 mM GTP—0.83—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseNeisseria meningitidisadenosine 5'-triphosphateATP varied at fixed 1.0 mM UMP; no GTP—3.22—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseNeisseria meningitidisadenosine 5'-triphosphateATP varied at fixed 1.0 mM UMP; 0.5 mM GTP—1.98—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseNeisseria meningitidisadenosine 5'-triphosphateno GTP—4.7—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseNeisseria meningitidisadenosine 5'-triphosphate0.5 mM GTP—1.7—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseNeisseria meningitidisuridine 5'-diphosphateno activator—0.0034—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseNeisseria meningitidisuridine 5'-diphosphate—0.0085—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseNeisseria meningitidisuridine 5'-monophosphateUMP varied at fixed 2.0 mM ATP; no GTP—0.0087—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseNeisseria meningitidisuridine 5'-monophosphateUMP varied at fixed 2.0 mM ATP; 0.5 mM GTP—0.01—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseNeisseria meningitidisuridine 5'-monophosphateUMP varied at fixed 10.0 mM ATP; no GTP—0.0156—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseNeisseria meningitidisuridine 5'-monophosphateUMP varied at fixed 10.0 mM ATP; 0.5 mM GTP—0.0574—pH 7.4, 30 °CPMID 17210578Open
UMP kinaseStreptococcus pneumoniaeATPMnCl2 (cation/NTP molar ratio 2)120——pH 7.5, 37 °CPMID 15324307Open
UMP kinaseStreptococcus pneumoniaeATPMgCl2 (cation/NTP molar ratio 2)175——pH 7.5, 37 °CPMID 15324307Open
UMP kinaseStreptococcus pneumoniaeuridine 5'-monophosphateMnCl2 (cation/NTP molar ratio 2)—0.15—pH 7.5, 37 °CPMID 15324307Open
UMP kinaseStreptococcus pneumoniaeuridine 5'-monophosphateMgCl2 (cation/NTP molar ratio 2)—0.1—pH 7.5, 37 °CPMID 15324307Open
UMP kinase (UMPKeco)variant D159NEscherichia coliATPUMP fixed at 0.3 mM; no GTP—0.19—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant D159NEscherichia coliATPUMP fixed at 1 mM; 0.5 mM GTP—0.2—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliATPUMP fixed at 0.3 mM; no GTP—0.36—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliATPUMP fixed at 1 mM; 0.5 mM GTP—0.41—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliATPUMP fixed at 0.3 mM; effector: no effector—0.27—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliATPUMP fixed at 0.3 mM; effector: 0.5 mM GTP—0.27—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliATPUMP fixed at 0.3 mM; effector: 0.5 mM UTP—0.64—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliATPUMP fixed at 0.3 mM; effector: 0.5 mM GTP + 0.5 mM UTP—0.59—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliUMPATP fixed at 2 mM; no GTP—0.0238—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliUMPATP fixed at 2 mM; 0.5 mM GTP—0.0244—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliUMPATP fixed at 0.2 mM; effector: no effector—0.0357—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliUMPATP fixed at 0.2 mM; effector: 0.5 mM GTP—0.0275—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliUMPATP fixed at 0.2 mM; effector: 0.5 mM UTP—0.0788—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliUMPATP fixed at 0.2 mM; effector: 0.5 mM GTP + 0.5 mM UTP—0.0755—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant N72A/D159NEscherichia coliATPUMP fixed at 0.3 mM; no GTP—0.59—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant N72A/D159NEscherichia coliATPUMP fixed at 1 mM; 0.5 mM GTP—0.4—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant N72A/D159NEscherichia coliUMPno GTP—0.0321—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant N72A/D159NEscherichia coliUMP0.5 mM GTP—0.0478—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant N72A/D93A/D159NEscherichia coliATPUMP fixed at 0.3 mM; no GTP—0.59—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant N72A/D93A/D159NEscherichia coliATPUMP fixed at 1 mM; 0.5 mM GTP—0.66—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant N72A/D93A/D159NEscherichia coliUMPno GTP—0.024—pH 7.4, 30 °CPMID 18945668Open
UMP kinase (UMPKeco)variant N72A/D93A/D159NEscherichia coliUMP0.5 mM GTP—0.0246—pH 7.4, 30 °CPMID 18945668Open