CatLogenzyme kinetics catalog

Values CatLog read from this paper

Structural and functional characterization of Escherichia coli UMP kinase in complex with its allosteric regulator GTP

PMID 18945668doi:10.1074/jbc.m802614200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 22 records (22 values) from this paper: UMP kinase (UMPKeco) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.4.22

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
UMP kinase (UMPKeco)variant D159NEscherichia coliATPUMP fixed at 0.3 mM; no GTP—0.19—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant D159NEscherichia coliATPUMP fixed at 1 mM; 0.5 mM GTP—0.2—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliATPUMP fixed at 0.3 mM; no GTP—0.36—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliATPUMP fixed at 1 mM; 0.5 mM GTP—0.41—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliATPUMP fixed at 0.3 mM; effector: no effector—0.27—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliATPUMP fixed at 0.3 mM; effector: 0.5 mM GTP—0.27—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliATPUMP fixed at 0.3 mM; effector: 0.5 mM UTP—0.64—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliATPUMP fixed at 0.3 mM; effector: 0.5 mM GTP + 0.5 mM UTP—0.59—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliUMPATP fixed at 2 mM; no GTP—0.0238—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliUMPATP fixed at 2 mM; 0.5 mM GTP—0.0244—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliUMPATP fixed at 0.2 mM; effector: no effector—0.0357—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliUMPATP fixed at 0.2 mM; effector: 0.5 mM GTP—0.0275—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliUMPATP fixed at 0.2 mM; effector: 0.5 mM UTP—0.0788—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant D93A/D159NEscherichia coliUMPATP fixed at 0.2 mM; effector: 0.5 mM GTP + 0.5 mM UTP—0.0755—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant N72A/D159NEscherichia coliATPUMP fixed at 0.3 mM; no GTP—0.59—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant N72A/D159NEscherichia coliATPUMP fixed at 1 mM; 0.5 mM GTP—0.4—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant N72A/D159NEscherichia coliUMPno GTP—0.0321—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant N72A/D159NEscherichia coliUMP0.5 mM GTP—0.0478—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant N72A/D93A/D159NEscherichia coliATPUMP fixed at 0.3 mM; no GTP—0.59—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant N72A/D93A/D159NEscherichia coliATPUMP fixed at 1 mM; 0.5 mM GTP—0.66—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant N72A/D93A/D159NEscherichia coliUMPno GTP—0.024—pH 7.4, 30 °COpen
UMP kinase (UMPKeco)variant N72A/D93A/D159NEscherichia coliUMP0.5 mM GTP—0.0246—pH 7.4, 30 °COpen