Values CatLog read from this paper
Crystal structure of Escherichia coli ketopantoate reductase in a ternary complex with NADP+ and pantoate bound: substrate recognition, conformational change, and cooperativity
PMID 17229734doi:10.1074/jbc.m611171200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (21 values) from this paper: ketopantoate reductase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ketopantoate reductase | Escherichia coli | ketopantoate | 25 | 0.03 | 830 calc. | pH 7.6, 25 °C | Open record: ketopantoate reductase, ketopantoate |
| ketopantoate reductase | Escherichia coli | NADPH | — | 0.0073 | — | pH 7.6, 25 °C | Open record: ketopantoate reductase, NADPH |
| ketopantoate reductasevariant E256A | Escherichia coli | ketopantoate | 2.1 | 35 | 0.060 calc. | pH 7.6, 25 °C | Open record: ketopantoate reductase variant E256A, ketopantoate |
| ketopantoate reductasevariant E256A | Escherichia coli | NADPH | — | 0.0026 | — | pH 7.6, 25 °C | Open record: ketopantoate reductase variant E256A, NADPH |
| ketopantoate reductasevariant K176A | Escherichia coli | ketopantoate | 0.015 | 44 | 3.4 × 10⁻⁴ calc. | pH 7.6, 25 °C | Open record: ketopantoate reductase variant K176A, ketopantoate |
| ketopantoate reductasevariant K176A | Escherichia coli | NADPH | — | 0.0047 | — | pH 7.6, 25 °C | Open record: ketopantoate reductase variant K176A, NADPH |
| ketopantoate reductasevariant K72A | Escherichia coli | ketopantoate | 42.8 | 1.7 | 25 calc. | pH 7.6, 25 °C | Open record: ketopantoate reductase variant K72A, ketopantoate |
| ketopantoate reductasevariant K72A | Escherichia coli | NADPH | — | 0.068 | — | pH 7.6, 25 °C | Open record: ketopantoate reductase variant K72A, NADPH |
| ketopantoate reductasevariant N98A | Escherichia coli | ketopantoate | 1.6 | 4.4 | 0.36 calc. | pH 7.6, 25 °C | Open record: ketopantoate reductase variant N98A, ketopantoate |
| ketopantoate reductasevariant N98A | Escherichia coli | NADPH | — | 0.0026 | — | pH 7.6, 25 °C | Open record: ketopantoate reductase variant N98A, NADPH |
| ketopantoate reductasevariant R31A | Escherichia coli | ketopantoate | 37 | 0.05 | 740 calc. | pH 7.6, 25 °C | Open record: ketopantoate reductase variant R31A, ketopantoate |
| ketopantoate reductasevariant R31A | Escherichia coli | NADPH | — | 0.03 | — | pH 7.6, 25 °C | Open record: ketopantoate reductase variant R31A, NADPH |
| ketopantoate reductasevariant S244A | Escherichia coli | ketopantoate | 15 | 7 | 2.1 calc. | pH 7.6, 25 °C | Open record: ketopantoate reductase variant S244A, ketopantoate |
| ketopantoate reductasevariant S244A | Escherichia coli | NADPH | — | 0.0023 | — | pH 7.6, 25 °C | Open record: ketopantoate reductase variant S244A, NADPH |