CatLogenzyme kinetics catalog

Values CatLog read from this paper

The role of Tyr605 and Ala607 of thimet oligopeptidase and Tyr606 and Gly608 of neurolysin in substrate hydrolysis and inhibitor binding

PMID 17313369doi:10.1042/bj20070060Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 82 records (221 values) from this paper: thimet oligopeptidase (TOP), neurolysin (NEL).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.4.24.15, EC 3.4.24.16

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
neurolysin (NEL)—o-aminobenzoyl-Gly-Phe-Ser-Trp-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.38 × 10⁻⁴375pH 7.4, 37 °COpen
neurolysin (NEL)—o-aminobenzoyl-Gly-Phe-Ser-Tyr-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine10.0096104pH 7.4, 37 °COpen
neurolysin (NEL)variant G608A—o-aminobenzoyl-Gly-Phe-Ser-Ala-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.92 × 10⁻⁴4.5 × 10³pH 7.4, 37 °COpen
neurolysin (NEL)variant G608A—o-aminobenzoyl-Gly-Phe-Ser-Arg-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine3.30.0083398pH 7.4, 37 °COpen
neurolysin (NEL)variant G608A—o-aminobenzoyl-Gly-Phe-Ser-Gln-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine5.90.00541093pH 7.4, 37 °COpen
neurolysin (NEL)variant G608A—o-aminobenzoyl-Gly-Phe-Ser-His-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine6.30.0095663pH 7.4, 37 °COpen
neurolysin (NEL)variant G608A—o-aminobenzoyl-Gly-Phe-Ser-Ile-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.048 × 10⁻⁴50pH 7.4, 37 °COpen
neurolysin (NEL)variant G608A—o-aminobenzoyl-Gly-Phe-Ser-Leu-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine2.40.00191263pH 7.4, 37 °COpen
neurolysin (NEL)variant G608A—o-aminobenzoyl-Gly-Phe-Ser-Phe-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine4.90.00251.96 × 10³pH 7.4, 37 °COpen
neurolysin (NEL)variant G608A—o-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.78 × 10⁻⁴875pH 7.4, 37 °COpen
neurolysin (NEL)variant G608A—o-aminobenzoyl-Gly-Phe-Ser-Ser-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine2.40.0056429pH 7.4, 37 °COpen
neurolysin (NEL)variant G608A—o-aminobenzoyl-Gly-Phe-Ser-Trp-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine2.40.0029828pH 7.4, 37 °COpen
neurolysin (NEL)variant G608A—o-aminobenzoyl-Gly-Phe-Ser-Tyr-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine4.10.00123417pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606A—o-aminobenzoyl-Gly-Phe-Ser-Ala-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.50.007864pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606A—o-aminobenzoyl-Gly-Phe-Ser-Arg-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.40.0026154pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606A—o-aminobenzoyl-Gly-Phe-Ser-Gln-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.70.02133pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606A—o-aminobenzoyl-Gly-Phe-Ser-Glu-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.10.005319pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606A—o-aminobenzoyl-Gly-Phe-Ser-His-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.050.01065pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606A—o-aminobenzoyl-Gly-Phe-Ser-Leu-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.10.006116pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606A—o-aminobenzoyl-Gly-Phe-Ser-Phe-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.090.01019pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606A—o-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamineoverall hydrolysis at both cleaved bonds (X–Phe and Phe–Ser)—0.003110pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606A—o-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediaminecleavage at the X–Phe bond only0.006——pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606A—o-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediaminecleavage at the Phe–Ser bond only0.024——pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606A—o-aminobenzoyl-Gly-Phe-Ser-Ser-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.060.00748pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606A—o-aminobenzoyl-Gly-Phe-Ser-Trp-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine10.0022455pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606A—o-aminobenzoyl-Gly-Phe-Ser-Tyr-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.30.006546pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606F—o-aminobenzoyl-Gly-Phe-Ser-Ala-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.80.0054148pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606F—o-aminobenzoyl-Gly-Phe-Ser-Arg-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.90.0019474pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606F—o-aminobenzoyl-Gly-Phe-Ser-Gln-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.60.009663pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606F—o-aminobenzoyl-Gly-Phe-Ser-Glu-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine1.20.005240pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606F—o-aminobenzoyl-Gly-Phe-Ser-His-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.20.010419pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606F—o-aminobenzoyl-Gly-Phe-Ser-Ile-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.010.00482pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606F—o-aminobenzoyl-Gly-Phe-Ser-Leu-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.70.0048146pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606F—o-aminobenzoyl-Gly-Phe-Ser-Phe-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine1.10.0098112pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606F—o-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.17 × 10⁻⁴143pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606F—o-aminobenzoyl-Gly-Phe-Ser-Ser-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine0.20.007427pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606F—o-aminobenzoyl-Gly-Phe-Ser-Trp-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine1.50.006250pH 7.4, 37 °COpen
neurolysin (NEL)variant Y606F—o-aminobenzoyl-Gly-Phe-Ser-Tyr-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine1.30.0038342pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)—o-aminobenzoyl-Gly-Phe-Ser-Trp-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine2.78 × 10⁻⁴3375pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)—o-aminobenzoyl-Gly-Phe-Ser-Tyr-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine6.29 × 10⁻⁴6889pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant A607G—o-aminobenzoyl-Gly-Phe-Ser-Ala-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine10.65 × 10⁻⁴2.12 × 10⁴pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant A607G—o-aminobenzoyl-Gly-Phe-Ser-Arg-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine9.87 × 10⁻⁴1.4 × 10⁴pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant A607G—o-aminobenzoyl-Gly-Phe-Ser-Gln-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine2.30.0034677pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant A607G—o-aminobenzoyl-Gly-Phe-Ser-His-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine10.69 × 10⁻⁴11,778pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant A607G—o-aminobenzoyl-Gly-Phe-Ser-Leu-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine3.80.00271407pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant A607G—o-aminobenzoyl-Gly-Phe-Ser-Phe-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine2.78 × 10⁻⁴3375pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant A607G—o-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine4.50.00133462pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant A607G—o-aminobenzoyl-Gly-Phe-Ser-Ser-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine12.10.00139308pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant A607G—o-aminobenzoyl-Gly-Phe-Ser-Trp-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine3.80.00192 × 10³pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant A607G—o-aminobenzoyl-Gly-Phe-Ser-Tyr-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine4.98 × 10⁻⁴6125pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Ala-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine10.40.00981061pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Arg-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamineoverall hydrolysis at both cleaved bonds (X–Phe and Phe–Ser)—0.01100pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Arg-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediaminecleavage at the X–Phe bond only0.6——pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Arg-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediaminecleavage at the Phe–Ser bond only0.4——pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Gln-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine5.80.00581 × 10³pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Glu-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine5.30.0031767pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-His-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine1.80.0096188pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Ile-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine50.0051 × 10³pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Leu-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamineoverall hydrolysis at both cleaved bonds (X–Phe and Phe–Ser)—0.01021745pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Leu-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediaminecleavage at the X–Phe bond only7——pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Leu-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediaminecleavage at the Phe–Ser bond only10.8——pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Phe-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine12.70.016794pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamineoverall hydrolysis at both cleaved bonds (X–Phe and Phe–Ser)—0.00431372pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediaminecleavage at the X–Phe bond only4.7——pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediaminecleavage at the Phe–Ser bond only1.2——pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Ser-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine1.50.0275pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Trp-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamineoverall hydrolysis at both cleaved bonds (X–Phe and Phe–Ser)—0.0074122pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Trp-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediaminecleavage at the X–Phe bond only0.2——pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Trp-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediaminecleavage at the Phe–Ser bond only0.7——pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605A—o-aminobenzoyl-Gly-Phe-Ser-Tyr-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine20.40.0111855pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605F—o-aminobenzoyl-Gly-Phe-Ser-Ala-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine2.60.0047553pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605F—o-aminobenzoyl-Gly-Phe-Ser-Arg-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine1.50.0044341pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605F—o-aminobenzoyl-Gly-Phe-Ser-Gln-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine12.10.00542241pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605F—o-aminobenzoyl-Gly-Phe-Ser-Glu-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine3.20.00211524pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605F—o-aminobenzoyl-Gly-Phe-Ser-His-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine15.10.00861756pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605F—o-aminobenzoyl-Gly-Phe-Ser-Ile-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine13.60.00149714pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605F—o-aminobenzoyl-Gly-Phe-Ser-Leu-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine13.60.00235913pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605F—o-aminobenzoyl-Gly-Phe-Ser-Phe-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine27.30.00338273pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605F—o-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine1.40.0015933pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605F—o-aminobenzoyl-Gly-Phe-Ser-Ser-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine1.70.0047362pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605F—o-aminobenzoyl-Gly-Phe-Ser-Trp-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine9.10.00156067pH 7.4, 37 °COpen
thimet oligopeptidase (TOP)variant Y605F—o-aminobenzoyl-Gly-Phe-Ser-Tyr-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine6.40.00232783pH 7.4, 37 °COpen