Values CatLog read from this paper

Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases

PMID 17323919doi:10.1021/bi061521nOpen in Browse

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CatLog read 26 records (78 values) from this paper: alpha-galactosidase TmGalA from Thermotoga maritima.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.2.1.22

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
alpha-galactosidase TmGalA	Thermotoga maritima	2,4-dinitrophenyl alpha-D-galactopyranoside	12.23	0.037	330.5	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA, 2,4-dinitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	2,5-dinitrophenyl alpha-D-galactopyranoside	11.2	0.143	78.3	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA, 2,5-dinitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	2-naphthyl alpha-D-galactopyranoside	6.26	0.039	160.5	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA, 2-naphthyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	2-nitrophenyl alpha-D-galactopyranoside	13.67	0.143	95.6	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA, 2-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	3,4-dimethylphenyl alpha-D-galactopyranoside	10.39	0.086	120.8	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA, 3,4-dimethylphenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	3-nitrophenyl alpha-D-galactopyranoside	10.5	0.096	109.4	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA, 3-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	4-bromophenyl alpha-D-galactopyranoside	12.58	0.111	113.3	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA, 4-bromophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	4-methoxyphenyl alpha-D-galactopyranoside	6.69	0.048	139.4	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA, 4-methoxyphenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranoside50 mM sodium acetate pH 5.0 containing 0.5 mg/mL BSA	8.58	0.08	107.3	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranosideno external nucleophile; 200 mM sodium citrate pH 5.0	33	0.052	620	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranosidepH 5.0 after addition of azide; Gal-PNP 12 µM to 2 mM	38	0.077	490	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranosidewith 2 M sodium formate buffer (external nucleophile)	31	0.044	700	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	phenyl alpha-D-galactopyranoside	5.71	0.176	32.4	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA, phenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	2,4-dinitrophenyl alpha-D-galactopyranoside	0.085	0.135	0.63	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA variant D327G, 2,4-dinitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	2,5-dinitrophenyl alpha-D-galactopyranoside	0.042	0.243	0.173	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA variant D327G, 2,5-dinitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	2-naphthyl alpha-D-galactopyranoside	0.021	0.225	0.093	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA variant D327G, 2-naphthyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	2-nitrophenyl alpha-D-galactopyranoside	0.035	0.133	0.263	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA variant D327G, 2-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	3,4-dimethylphenyl alpha-D-galactopyranoside	0.01	0.089	0.112	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA variant D327G, 3,4-dimethylphenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	3-nitrophenyl alpha-D-galactopyranoside	0.042	0.14	0.3	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA variant D327G, 3-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	4-bromophenyl alpha-D-galactopyranoside	0.016	0.104	0.154	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA variant D327G, 4-bromophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	4-methoxyphenyl alpha-D-galactopyranoside	0.01	0.088	0.114	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA variant D327G, 4-methoxyphenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranoside	0.03	0.083	0.361	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA variant D327G, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	phenyl alpha-D-galactopyranoside	0.027	0.324	0.083	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA variant D327G, phenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D387G	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranosideno external nucleophile; 200 mM sodium citrate pH 5.0	0.022	0.036	0.61	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA variant D387G, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D387G	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranosidepH 5.0 after addition of azide	0.027	0.074	0.36	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA variant D387G, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D387G	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranosidewith 2 M sodium formate buffer (external nucleophile)	0.031	0.04	0.77	pH 5, 37 °C	Open record: alpha-galactosidase TmGalA variant D387G, 4-nitrophenyl alpha-D-galactopyranoside