EC 3.2.1.22: alpha-galactosidase

HydrolasesOpen in Browse

CatLog read 82 records for EC 3.2.1.22 from 8 papers, for 7 organisms. Names in these papers: alpha-galactosidase TmGalA, α-galactosidase AgaA, alpha-D-galactosidase, α-galactosidase (ScAGal) and 7 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 141 records for EC 3.2.1.22 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.22 in Browse.

Papers

Papers with records for EC 3.2.1.22
Paper	Organisms	Records
Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases	Thermotoga maritima	26
The molecular mechanism of thermostable α-galactosidases AgaA and AgaB explained by x-ray crystallography and mutational studies	Geobacillus stearothermophilus	13
Effects of several alcohols on glycosidase activities in rat liver and brain	Rattus norvegicus	10
Purification and characterization of two alpha-galactosidases associated with catabolism of guar gum and other alpha-galactosides by Bacteroides ovatus	Bacteroides ovatus	10
Structural analysis of Saccharomyces cerevisiae alpha-galactosidase and its complexes with natural substrates reveals new insights into substrate specificity of GH27 glycosidases	Saccharomyces cerevisiae	9
Characterization of alpha-Galactosidase from Cucumber Leaves	Cucumis sativus	8
Catalytic mechanism of retaining alpha-galactosidase belonging to glycoside hydrolase family 97	Bacteroides thetaiotaomicron	4
Divergence of catalytic mechanism within a glycosidase family provides insight into evolution of carbohydrate metabolism by human gut flora	Bacteroides thetaiotaomicron	2

Records

Records for EC 3.2.1.22 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
alpha-D-galactosidase	Rattus norvegicus	4-nitrophenyl alpha-D-galactopyranosideliver; Table 2	—	3.56	—	pH 4.5, 37 °C	PMID 1499277	Open record: alpha-D-galactosidase, 4-nitrophenyl alpha-D-galactopyranoside
alpha-D-galactosidase	Rattus norvegicus	4-nitrophenyl alpha-D-galactopyranosideliver; Table 2	—	3.55	—	pH 4.5, 37 °C	PMID 1499277	Open record: alpha-D-galactosidase, 4-nitrophenyl alpha-D-galactopyranoside
alpha-D-galactosidase	Rattus norvegicus	4-nitrophenyl alpha-D-galactopyranosideliver; Table 2	—	3.35	—	pH 4.5, 37 °C	PMID 1499277	Open record: alpha-D-galactosidase, 4-nitrophenyl alpha-D-galactopyranoside
alpha-D-galactosidase	Rattus norvegicus	4-nitrophenyl alpha-D-galactopyranosideliver; Table 2	—	3.75	—	pH 4.5, 37 °C	PMID 1499277	Open record: alpha-D-galactosidase, 4-nitrophenyl alpha-D-galactopyranoside
alpha-D-galactosidase	Rattus norvegicus	4-nitrophenyl alpha-D-galactopyranosideliver; Table 2	—	3.53	—	pH 4.5, 37 °C	PMID 1499277	Open record: alpha-D-galactosidase, 4-nitrophenyl alpha-D-galactopyranoside
alpha-D-galactosidase	Rattus norvegicus	4-nitrophenyl alpha-D-galactopyranosidebrain; Table 4	—	3.51	—	pH 4.5, 37 °C	PMID 1499277	Open record: alpha-D-galactosidase, 4-nitrophenyl alpha-D-galactopyranoside
alpha-D-galactosidase	Rattus norvegicus	4-nitrophenyl alpha-D-galactopyranosidebrain; Table 4	—	3.57	—	pH 4.5, 37 °C	PMID 1499277	Open record: alpha-D-galactosidase, 4-nitrophenyl alpha-D-galactopyranoside
alpha-D-galactosidase	Rattus norvegicus	4-nitrophenyl alpha-D-galactopyranosidebrain; Table 4	—	3.57	—	pH 4.5, 37 °C	PMID 1499277	Open record: alpha-D-galactosidase, 4-nitrophenyl alpha-D-galactopyranoside
alpha-D-galactosidase	Rattus norvegicus	4-nitrophenyl alpha-D-galactopyranosidebrain; Table 4	—	3.6	—	pH 4.5, 37 °C	PMID 1499277	Open record: alpha-D-galactosidase, 4-nitrophenyl alpha-D-galactopyranoside
alpha-D-galactosidase	Rattus norvegicus	4-nitrophenyl alpha-D-galactopyranosidebrain; Table 4	—	3.56	—	pH 4.5, 37 °C	PMID 1499277	Open record: alpha-D-galactosidase, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase I	Bacteroides ovatus	2-nitrophenyl alpha-D-galactopyranoside	—	0.2	—		PMID 2981815	Open record: alpha-galactosidase I, 2-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase I	Bacteroides ovatus	4-nitrophenyl alpha-D-galactopyranoside	—	0.2	—	pH 6.3, 37 °C	PMID 2981815	Open record: alpha-galactosidase I, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase I	Bacteroides ovatus	melibiose	—	20.8	—	pH 6.3, 37 °C	PMID 2981815	Open record: alpha-galactosidase I, melibiose
alpha-galactosidase I	Bacteroides ovatus	raffinose	—	98.1	—	pH 6.3, 37 °C	PMID 2981815	Open record: alpha-galactosidase I, raffinose
alpha-galactosidase I	Bacteroides ovatus	stachyose	—	8.5	—	pH 6.3, 37 °C	PMID 2981815	Open record: alpha-galactosidase I, stachyose
alpha-galactosidase II	Bacteroides ovatus	2-nitrophenyl alpha-D-galactopyranoside	—	0.3	—		PMID 2981815	Open record: alpha-galactosidase II, 2-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase II	Bacteroides ovatus	4-nitrophenyl alpha-D-galactopyranoside	—	0.4	—	pH 6.3, 37 °C	PMID 2981815	Open record: alpha-galactosidase II, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase II	Bacteroides ovatus	melibiose	—	2.3	—	pH 6.3, 37 °C	PMID 2981815	Open record: alpha-galactosidase II, melibiose
alpha-galactosidase II	Bacteroides ovatus	raffinose	—	5.9	—	pH 6.3, 37 °C	PMID 2981815	Open record: alpha-galactosidase II, raffinose
alpha-galactosidase II	Bacteroides ovatus	stachyose	—	0.3	—	pH 6.3, 37 °C	PMID 2981815	Open record: alpha-galactosidase II, stachyose
alpha-galactosidase TmGalA	Thermotoga maritima	2,4-dinitrophenyl alpha-D-galactopyranoside	12.23	0.037	330.5	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA, 2,4-dinitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	2,5-dinitrophenyl alpha-D-galactopyranoside	11.2	0.143	78.3	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA, 2,5-dinitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	2-naphthyl alpha-D-galactopyranoside	6.26	0.039	160.5	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA, 2-naphthyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	2-nitrophenyl alpha-D-galactopyranoside	13.67	0.143	95.6	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA, 2-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	3,4-dimethylphenyl alpha-D-galactopyranoside	10.39	0.086	120.8	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA, 3,4-dimethylphenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	3-nitrophenyl alpha-D-galactopyranoside	10.5	0.096	109.4	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA, 3-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	4-bromophenyl alpha-D-galactopyranoside	12.58	0.111	113.3	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA, 4-bromophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	4-methoxyphenyl alpha-D-galactopyranoside	6.69	0.048	139.4	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA, 4-methoxyphenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranoside50 mM sodium acetate pH 5.0 containing 0.5 mg/mL BSA	8.58	0.08	107.3	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranosideno external nucleophile; 200 mM sodium citrate pH 5.0	33	0.052	620	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranosidepH 5.0 after addition of azide; Gal-PNP 12 µM to 2 mM	38	0.077	490	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranosidewith 2 M sodium formate buffer (external nucleophile)	31	0.044	700	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalA	Thermotoga maritima	phenyl alpha-D-galactopyranoside	5.71	0.176	32.4	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA, phenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	2,4-dinitrophenyl alpha-D-galactopyranoside	0.085	0.135	0.63	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA variant D327G, 2,4-dinitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	2,5-dinitrophenyl alpha-D-galactopyranoside	0.042	0.243	0.173	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA variant D327G, 2,5-dinitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	2-naphthyl alpha-D-galactopyranoside	0.021	0.225	0.093	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA variant D327G, 2-naphthyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	2-nitrophenyl alpha-D-galactopyranoside	0.035	0.133	0.263	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA variant D327G, 2-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	3,4-dimethylphenyl alpha-D-galactopyranoside	0.01	0.089	0.112	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA variant D327G, 3,4-dimethylphenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	3-nitrophenyl alpha-D-galactopyranoside	0.042	0.14	0.3	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA variant D327G, 3-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	4-bromophenyl alpha-D-galactopyranoside	0.016	0.104	0.154	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA variant D327G, 4-bromophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	4-methoxyphenyl alpha-D-galactopyranoside	0.01	0.088	0.114	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA variant D327G, 4-methoxyphenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranoside	0.03	0.083	0.361	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA variant D327G, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D327G	Thermotoga maritima	phenyl alpha-D-galactopyranoside	0.027	0.324	0.083	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA variant D327G, phenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D387G	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranosideno external nucleophile; 200 mM sodium citrate pH 5.0	0.022	0.036	0.61	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA variant D387G, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D387G	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranosidepH 5.0 after addition of azide	0.027	0.074	0.36	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA variant D387G, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase TmGalAvariant D387G	Thermotoga maritima	4-nitrophenyl alpha-D-galactopyranosidewith 2 M sodium formate buffer (external nucleophile)	0.031	0.04	0.77	pH 5, 37 °C	PMID 17323919	Open record: alpha-galactosidase TmGalA variant D387G, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase, 25,000-mol wt form	Cucumis sativus	4-nitrophenyl alpha-D-galactopyranoside	—	0.32	—	pH 5.5, 30 °C	PMID 16661511	Open record: alpha-galactosidase, 25,000-mol wt form, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase, 25,000-mol wt form	Cucumis sativus	melibiose	—	3.5	—	pH 5.5, 30 °C	PMID 16661511	Open record: alpha-galactosidase, 25,000-mol wt form, melibiose
alpha-galactosidase, 25,000-mol wt form	Cucumis sativus	raffinose	—	5	—	pH 5.5, 30 °C	PMID 16661511	Open record: alpha-galactosidase, 25,000-mol wt form, raffinose
alpha-galactosidase, 25,000-mol wt form	Cucumis sativus	stachyose	—	30	—	pH 5.5, 30 °C	PMID 16661511	Open record: alpha-galactosidase, 25,000-mol wt form, stachyose
alpha-galactosidase, 50,000-mol wt form	Cucumis sativus	4-nitrophenyl alpha-D-galactopyranoside	—	0.22	—	pH 5.2, 30 °C	PMID 16661511	Open record: alpha-galactosidase, 50,000-mol wt form, 4-nitrophenyl alpha-D-galactopyranoside
alpha-galactosidase, 50,000-mol wt form	Cucumis sativus	melibiose	—	3	—	pH 5.2, 30 °C	PMID 16661511	Open record: alpha-galactosidase, 50,000-mol wt form, melibiose
alpha-galactosidase, 50,000-mol wt form	Cucumis sativus	raffinose	—	6.5	—	pH 5.2, 30 °C	PMID 16661511	Open record: alpha-galactosidase, 50,000-mol wt form, raffinose
alpha-galactosidase, 50,000-mol wt form	Cucumis sativus	stachyose	—	10	—	pH 5.2, 30 °C	PMID 16661511	Open record: alpha-galactosidase, 50,000-mol wt form, stachyose
BtGH97b (GH97 alpha-galactosidase)	Bacteroides thetaiotaomicron	4-nitrophenyl alpha-D-galactopyranoside	91	0.23	391	pH 6.6, 37 °C	PMID 18848471	Open record: BtGH97b (GH97 alpha-galactosidase), 4-nitrophenyl alpha-D-galactopyranoside
BtGH97b (GH97 alpha-galactosidase)	Bacteroides thetaiotaomicron	melibiose	0.015	1.5	0.011	pH 6.6, 37 °C	PMID 18848471	Open record: BtGH97b (GH97 alpha-galactosidase), melibiose
BtGH97b (retaining alpha-galactosidase, glycoside hydrolase family 97)	Bacteroides thetaiotaomicron	p-nitrophenyl alpha-D-galactopyranoside	244	0.31	778	pH 6, 35 °C	PMID 19646996	Open record: BtGH97b (retaining alpha-galactosidase, glycoside hydrolase family 97), p-nitrophenyl alpha-D-galactopyranoside
BtGH97b (retaining alpha-galactosidase, glycoside hydrolase family 97)variant D415G	Bacteroides thetaiotaomicron	p-nitrophenyl alpha-D-galactopyranoside	0.039	0.033	86.5	pH 6	PMID 19646996	Open record: BtGH97b (retaining alpha-galactosidase, glycoside hydrolase family 97) variant D415G, p-nitrophenyl alpha-D-galactopyranoside
BtGH97b (retaining alpha-galactosidase, glycoside hydrolase family 97)variant D415N	Bacteroides thetaiotaomicron	p-nitrophenyl alpha-D-galactopyranoside	0.00218	0.19	0.0115	pH 6, 35 °C	PMID 19646996	Open record: BtGH97b (retaining alpha-galactosidase, glycoside hydrolase family 97) variant D415N, p-nitrophenyl alpha-D-galactopyranoside
BtGH97b (retaining alpha-galactosidase, glycoside hydrolase family 97)variant E470Q	Bacteroides thetaiotaomicron	p-nitrophenyl alpha-D-galactopyranoside	0.0684	0.32	0.213	pH 6, 35 °C	PMID 19646996	Open record: BtGH97b (retaining alpha-galactosidase, glycoside hydrolase family 97) variant E470Q, p-nitrophenyl alpha-D-galactopyranoside
α-galactosidase (ScAGal)	Saccharomyces cerevisiae	melibiose	—	11.2	—	pH 4, 30 °C	PMID 20592022	Open record: α-galactosidase (ScAGal), melibiose
α-galactosidase (ScAGal)	Saccharomyces cerevisiae	p-nitrophenyl-α-D-galactopyranoside	—	4.5	—	pH 4, 30 °C	PMID 20592022	Open record: α-galactosidase (ScAGal), p-nitrophenyl-α-D-galactopyranoside
α-galactosidase (ScAGal)	Saccharomyces cerevisiae	raffinose	—	54.1	—	pH 4, 30 °C	PMID 20592022	Open record: α-galactosidase (ScAGal), raffinose
α-galactosidase (ScAGal)variant A41Y	Saccharomyces cerevisiae	melibiose	—	5.8	—	pH 4, 30 °C	PMID 20592022	Open record: α-galactosidase (ScAGal) variant A41Y, melibiose
α-galactosidase (ScAGal)variant A41Y	Saccharomyces cerevisiae	p-nitrophenyl-α-D-galactopyranoside	—	3.7	—	pH 4, 30 °C	PMID 20592022	Open record: α-galactosidase (ScAGal) variant A41Y, p-nitrophenyl-α-D-galactopyranoside
α-galactosidase (ScAGal)variant A41Y	Saccharomyces cerevisiae	raffinose	—	87.9	—	pH 4, 30 °C	PMID 20592022	Open record: α-galactosidase (ScAGal) variant A41Y, raffinose
α-galactosidase (ScAGal)variant Q251A	Saccharomyces cerevisiae	melibiose	—	254	—	pH 4, 30 °C	PMID 20592022	Open record: α-galactosidase (ScAGal) variant Q251A, melibiose
α-galactosidase (ScAGal)variant Q251A	Saccharomyces cerevisiae	p-nitrophenyl-α-D-galactopyranoside	—	1.4	—	pH 4, 30 °C	PMID 20592022	Open record: α-galactosidase (ScAGal) variant Q251A, p-nitrophenyl-α-D-galactopyranoside
α-galactosidase (ScAGal)variant Q251W	Saccharomyces cerevisiae	p-nitrophenyl-α-D-galactopyranoside	—	0.24	—	pH 4, 30 °C	PMID 20592022	Open record: α-galactosidase (ScAGal) variant Q251W, p-nitrophenyl-α-D-galactopyranoside
α-galactosidase AgaA	Geobacillus stearothermophilus	para-nitrophenyl α-D-galactopyranoside	105.6	0.21	502	pH 6.5, 25 °C	PMID 23012371	Open record: α-galactosidase AgaA, para-nitrophenyl α-D-galactopyranoside
α-galactosidase AgaA	Geobacillus stearothermophilus	raffinose	180	3.8	64.3	pH 6.5, 25 °C	PMID 23012371	Open record: α-galactosidase AgaA, raffinose
α-galactosidase AgaAvariant W336A	Geobacillus stearothermophilus	para-nitrophenyl α-D-galactopyranoside	7.9	0.44	17.9	pH 6.5, 25 °C	PMID 23012371	Open record: α-galactosidase AgaA variant W336A, para-nitrophenyl α-D-galactopyranoside
α-galactosidase AgaAvariant W336A	Geobacillus stearothermophilus	raffinose	48.1	252	0.19	pH 6.5, 25 °C	PMID 23012371	Open record: α-galactosidase AgaA variant W336A, raffinose
α-galactosidase AgaAvariant W336D	Geobacillus stearothermophilus	para-nitrophenyl α-D-galactopyranoside	0.97	1.33	0.7	pH 6.5, 25 °C	PMID 23012371	Open record: α-galactosidase AgaA variant W336D, para-nitrophenyl α-D-galactopyranoside
α-galactosidase AgaAvariant W336F	Geobacillus stearothermophilus	para-nitrophenyl α-D-galactopyranoside	375	0.24	1562	pH 6.5, 25 °C	PMID 23012371	Open record: α-galactosidase AgaA variant W336F, para-nitrophenyl α-D-galactopyranoside
α-galactosidase AgaAvariant W336F	Geobacillus stearothermophilus	raffinose	832	250	3.3	pH 6.5, 25 °C	PMID 23012371	Open record: α-galactosidase AgaA variant W336F, raffinose
α-galactosidase AgaAvariant W336N	Geobacillus stearothermophilus	para-nitrophenyl α-D-galactopyranoside	38.2	0.84	45.5	pH 6.5, 25 °C	PMID 23012371	Open record: α-galactosidase AgaA variant W336N, para-nitrophenyl α-D-galactopyranoside
α-galactosidase AgaAvariant W336N	Geobacillus stearothermophilus	raffinose	10.3	205	0.05	pH 6.5, 25 °C	PMID 23012371	Open record: α-galactosidase AgaA variant W336N, raffinose
α-galactosidase AgaAvariant W336S	Geobacillus stearothermophilus	para-nitrophenyl α-D-galactopyranoside	48	8.7	5.5	pH 6.5, 25 °C	PMID 23012371	Open record: α-galactosidase AgaA variant W336S, para-nitrophenyl α-D-galactopyranoside
α-galactosidase AgaAvariant W336S	Geobacillus stearothermophilus	raffinose	5.2	36.6	0.14	pH 6.5, 25 °C	PMID 23012371	Open record: α-galactosidase AgaA variant W336S, raffinose
α-galactosidase AgaB	Geobacillus stearothermophilus	para-nitrophenyl α-D-galactopyranoside	2382	0.83	2.87 × 10³	pH 6.5, 25 °C	PMID 23012371	Open record: α-galactosidase AgaB, para-nitrophenyl α-D-galactopyranoside
α-galactosidase AgaB	Geobacillus stearothermophilus	raffinose	1205	200	6	pH 6.5, 25 °C	PMID 23012371	Open record: α-galactosidase AgaB, raffinose