Values CatLog read from this paper
The molecular mechanism of thermostable α-galactosidases AgaA and AgaB explained by x-ray crystallography and mutational studies
PMID 23012371doi:10.1074/jbc.m112.394114Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (39 values) from this paper: α-galactosidase AgaA, α-galactosidase AgaB from Geobacillus stearothermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| α-galactosidase AgaA | Geobacillus stearothermophilus | para-nitrophenyl α-D-galactopyranoside | 105.6 | 0.21 | 502 | pH 6.5, 25 °C | Open record: α-galactosidase AgaA, para-nitrophenyl α-D-galactopyranoside |
| α-galactosidase AgaA | Geobacillus stearothermophilus | raffinose | 180 | 3.8 | 64.3 | pH 6.5, 25 °C | Open record: α-galactosidase AgaA, raffinose |
| α-galactosidase AgaAvariant W336A | Geobacillus stearothermophilus | para-nitrophenyl α-D-galactopyranoside | 7.9 | 0.44 | 17.9 | pH 6.5, 25 °C | Open record: α-galactosidase AgaA variant W336A, para-nitrophenyl α-D-galactopyranoside |
| α-galactosidase AgaAvariant W336A | Geobacillus stearothermophilus | raffinose | 48.1 | 252 | 0.19 | pH 6.5, 25 °C | Open record: α-galactosidase AgaA variant W336A, raffinose |
| α-galactosidase AgaAvariant W336D | Geobacillus stearothermophilus | para-nitrophenyl α-D-galactopyranoside | 0.97 | 1.33 | 0.7 | pH 6.5, 25 °C | Open record: α-galactosidase AgaA variant W336D, para-nitrophenyl α-D-galactopyranoside |
| α-galactosidase AgaAvariant W336F | Geobacillus stearothermophilus | para-nitrophenyl α-D-galactopyranoside | 375 | 0.24 | 1562 | pH 6.5, 25 °C | Open record: α-galactosidase AgaA variant W336F, para-nitrophenyl α-D-galactopyranoside |
| α-galactosidase AgaAvariant W336F | Geobacillus stearothermophilus | raffinose | 832 | 250 | 3.3 | pH 6.5, 25 °C | Open record: α-galactosidase AgaA variant W336F, raffinose |
| α-galactosidase AgaAvariant W336N | Geobacillus stearothermophilus | para-nitrophenyl α-D-galactopyranoside | 38.2 | 0.84 | 45.5 | pH 6.5, 25 °C | Open record: α-galactosidase AgaA variant W336N, para-nitrophenyl α-D-galactopyranoside |
| α-galactosidase AgaAvariant W336N | Geobacillus stearothermophilus | raffinose | 10.3 | 205 | 0.05 | pH 6.5, 25 °C | Open record: α-galactosidase AgaA variant W336N, raffinose |
| α-galactosidase AgaAvariant W336S | Geobacillus stearothermophilus | para-nitrophenyl α-D-galactopyranoside | 48 | 8.7 | 5.5 | pH 6.5, 25 °C | Open record: α-galactosidase AgaA variant W336S, para-nitrophenyl α-D-galactopyranoside |
| α-galactosidase AgaAvariant W336S | Geobacillus stearothermophilus | raffinose | 5.2 | 36.6 | 0.14 | pH 6.5, 25 °C | Open record: α-galactosidase AgaA variant W336S, raffinose |
| α-galactosidase AgaB | Geobacillus stearothermophilus | para-nitrophenyl α-D-galactopyranoside | 2382 | 0.83 | 2.87 × 10³ | pH 6.5, 25 °C | Open record: α-galactosidase AgaB, para-nitrophenyl α-D-galactopyranoside |
| α-galactosidase AgaB | Geobacillus stearothermophilus | raffinose | 1205 | 200 | 6 | pH 6.5, 25 °C | Open record: α-galactosidase AgaB, raffinose |