Values CatLog read from this paper
Structural analysis of Saccharomyces cerevisiae alpha-galactosidase and its complexes with natural substrates reveals new insights into substrate specificity of GH27 glycosidases
PMID 20592022doi:10.1074/jbc.m110.144584Open in Browse
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CatLog read 9 records (9 values) from this paper: α-galactosidase (ScAGal) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| α-galactosidase (ScAGal) | Saccharomyces cerevisiae | melibiose | — | 11.2 | — | pH 4, 30 °C | Open record: α-galactosidase (ScAGal), melibiose |
| α-galactosidase (ScAGal) | Saccharomyces cerevisiae | p-nitrophenyl-α-D-galactopyranoside | — | 4.5 | — | pH 4, 30 °C | Open record: α-galactosidase (ScAGal), p-nitrophenyl-α-D-galactopyranoside |
| α-galactosidase (ScAGal) | Saccharomyces cerevisiae | raffinose | — | 54.1 | — | pH 4, 30 °C | Open record: α-galactosidase (ScAGal), raffinose |
| α-galactosidase (ScAGal)variant A41Y | Saccharomyces cerevisiae | melibiose | — | 5.8 | — | pH 4, 30 °C | Open record: α-galactosidase (ScAGal) variant A41Y, melibiose |
| α-galactosidase (ScAGal)variant A41Y | Saccharomyces cerevisiae | p-nitrophenyl-α-D-galactopyranoside | — | 3.7 | — | pH 4, 30 °C | Open record: α-galactosidase (ScAGal) variant A41Y, p-nitrophenyl-α-D-galactopyranoside |
| α-galactosidase (ScAGal)variant A41Y | Saccharomyces cerevisiae | raffinose | — | 87.9 | — | pH 4, 30 °C | Open record: α-galactosidase (ScAGal) variant A41Y, raffinose |
| α-galactosidase (ScAGal)variant Q251A | Saccharomyces cerevisiae | melibiose | — | 254 | — | pH 4, 30 °C | Open record: α-galactosidase (ScAGal) variant Q251A, melibiose |
| α-galactosidase (ScAGal)variant Q251A | Saccharomyces cerevisiae | p-nitrophenyl-α-D-galactopyranoside | — | 1.4 | — | pH 4, 30 °C | Open record: α-galactosidase (ScAGal) variant Q251A, p-nitrophenyl-α-D-galactopyranoside |
| α-galactosidase (ScAGal)variant Q251W | Saccharomyces cerevisiae | p-nitrophenyl-α-D-galactopyranoside | — | 0.24 | — | pH 4, 30 °C | Open record: α-galactosidase (ScAGal) variant Q251W, p-nitrophenyl-α-D-galactopyranoside |