Values CatLog read from this paper
Divergence of catalytic mechanism within a glycosidase family provides insight into evolution of carbohydrate metabolism by human gut flora
PMID 18848471doi:10.1016/j.chembiol.2008.09.005Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (26 values) from this paper: BtGH97a (SusB, GH97 alpha-glucosidase), BtGH97b (GH97 alpha-galactosidase) from Bacteroides thetaiotaomicron.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| BtGH97a (SusB, GH97 alpha-glucosidase) | Bacteroides thetaiotaomicron | 2,4-dinitrophenyl alpha-D-glucopyranosidecontinuous spectrophotometric assay | 280 | 0.14 | 1997 | pH 6.6, 37 °C | Open record: BtGH97a (SusB, GH97 alpha-glucosidase), 2,4-dinitrophenyl alpha-D-glucopyranoside |
| BtGH97a (SusB, GH97 alpha-glucosidase) | Bacteroides thetaiotaomicron | 2,4-dinitrophenyl alpha-D-glucopyranosidekcat/Km determined directly | — | — | 1807 | pH 6.6, 37 °C | Open record: BtGH97a (SusB, GH97 alpha-glucosidase), 2,4-dinitrophenyl alpha-D-glucopyranoside |
| BtGH97a (SusB, GH97 alpha-glucosidase) | Bacteroides thetaiotaomicron | 4-nitrophenyl alpha-D-glucopyranoside | 53 | 0.085 | 620 | pH 6.6, 37 °C | Open record: BtGH97a (SusB, GH97 alpha-glucosidase), 4-nitrophenyl alpha-D-glucopyranoside |
| BtGH97a (SusB, GH97 alpha-glucosidase) | Bacteroides thetaiotaomicron | maltose | 0.015 | 2 | 0.0072 | pH 6.6, 37 °C | Open record: BtGH97a (SusB, GH97 alpha-glucosidase), maltose |
| BtGH97a (SusB, GH97 alpha-glucosidase)variant E194A | Bacteroides thetaiotaomicron | 2,4-dinitrophenyl alpha-D-glucopyranoside | — | — | 255 | pH 6.6, 37 °C | Open record: BtGH97a (SusB, GH97 alpha-glucosidase) variant E194A, 2,4-dinitrophenyl alpha-D-glucopyranoside |
| BtGH97a (SusB, GH97 alpha-glucosidase)variant E194A | Bacteroides thetaiotaomicron | 4-nitrophenyl alpha-D-glucopyranoside | 2.8 | 0.31 | 9.2 | pH 6.6, 37 °C | Open record: BtGH97a (SusB, GH97 alpha-glucosidase) variant E194A, 4-nitrophenyl alpha-D-glucopyranoside |
| BtGH97a (SusB, GH97 alpha-glucosidase)variant E439A | Bacteroides thetaiotaomicron | 2,4-dinitrophenyl alpha-D-glucopyranoside | — | — | 0.007 | pH 6.6, 37 °C | Open record: BtGH97a (SusB, GH97 alpha-glucosidase) variant E439A, 2,4-dinitrophenyl alpha-D-glucopyranoside |
| BtGH97a (SusB, GH97 alpha-glucosidase)variant E526A | Bacteroides thetaiotaomicron | 2,4-dinitrophenyl alpha-D-glucopyranoside | — | — | 48 | pH 6.6, 37 °C | Open record: BtGH97a (SusB, GH97 alpha-glucosidase) variant E526A, 2,4-dinitrophenyl alpha-D-glucopyranoside |
| BtGH97a (SusB, GH97 alpha-glucosidase)variant E532A | Bacteroides thetaiotaomicron | 2,4-dinitrophenyl alpha-D-glucopyranoside | — | — | 402 | pH 6.6, 37 °C | Open record: BtGH97a (SusB, GH97 alpha-glucosidase) variant E532A, 2,4-dinitrophenyl alpha-D-glucopyranoside |
| BtGH97a (SusB, GH97 alpha-glucosidase)variant E532A | Bacteroides thetaiotaomicron | 4-nitrophenyl alpha-D-glucopyranoside | 1.1 | 0.14 | 7.7 | pH 6.6, 37 °C | Open record: BtGH97a (SusB, GH97 alpha-glucosidase) variant E532A, 4-nitrophenyl alpha-D-glucopyranoside |
| BtGH97b (GH97 alpha-galactosidase) | Bacteroides thetaiotaomicron | 4-nitrophenyl alpha-D-galactopyranoside | 91 | 0.23 | 391 | pH 6.6, 37 °C | Open record: BtGH97b (GH97 alpha-galactosidase), 4-nitrophenyl alpha-D-galactopyranoside |
| BtGH97b (GH97 alpha-galactosidase) | Bacteroides thetaiotaomicron | melibiose | 0.015 | 1.5 | 0.011 | pH 6.6, 37 °C | Open record: BtGH97b (GH97 alpha-galactosidase), melibiose |