Values CatLog read from this paper
The Helicobacter pylori amidotransferase GatCAB is equally efficient in glutamine-dependent transamidation of Asp-tRNAAsn and Glu-tRNAGln
PMID 17329242doi:10.1074/jbc.m700398200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (33 values) from this paper: GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase) from Helicobacter pylori.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase) | Helicobacter pylori | adenosine 5'-triphosphate | 6.1 | 0.2068 | 29.5 | pH 7.2, 37 °C | Open record: GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase), adenosine 5'-triphosphate |
| GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase) | Helicobacter pylori | L-asparagine | 0.027 | 0.0224 | 1.2 | pH 7.2, 37 °C | Open record: GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase), L-asparagine |
| GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase) | Helicobacter pylori | L-aspartyl-tRNA(Asn) (H. pylori tRNAAsn) | 1.3 | 9.5 × 10⁻⁴ | 1368 | pH 7.2, 37 °C | Open record: GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase), L-aspartyl-tRNA(Asn) (H. pylori tRNAAsn) |
| GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase) | Helicobacter pylori | L-glutamineamidotransferase (transamidation) assay; 1 nM enzyme | 3.49 | 0.0207 | 168.6 | pH 7.2, 37 °C | Open record: GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase), L-glutamine |
| GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase) | Helicobacter pylori | L-glutamineglutaminase assay (L-[14C]Gln -> Glu, cellulose TLC); no ATP | 0.34 | 0.0265 | 12.8 | pH 7.2, 37 °C | Open record: GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase), L-glutamine |
| GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase) | Helicobacter pylori | L-glutamineglutaminase assay (L-[14C]Gln -> Glu, cellulose TLC) | 0.2 | 0.0119 | 16.8 | pH 7.2, 37 °C | Open record: GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase), L-glutamine |
| GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase) | Helicobacter pylori | L-glutamineglutaminase assay (L-[14C]Gln -> Glu, cellulose TLC); no ATP | 0.97 | 0.0383 | 25.3 | pH 7.2, 37 °C | Open record: GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase), L-glutamine |
| GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase) | Helicobacter pylori | L-glutamineglutaminase assay (L-[14C]Gln -> Glu, cellulose TLC); no ATP | 1.32 | 0.0557 | 23.7 | pH 7.2, 37 °C | Open record: GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase), L-glutamine |
| GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase) | Helicobacter pylori | L-glutamineglutaminase assay (L-[14C]Gln -> Glu, cellulose TLC) | 11.8 | 0.0402 | 293.5 | pH 7.2, 37 °C | Open record: GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase), L-glutamine |
| GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase) | Helicobacter pylori | L-glutamineglutaminase assay (L-[14C]Gln -> Glu, cellulose TLC) | 10.29 | 0.0509 | 202.2 | pH 7.2, 37 °C | Open record: GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase), L-glutamine |
| GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase) | Helicobacter pylori | L-glutamyl-tRNA(Gln) (H. pylori tRNAGln) | 3.61 | 0.00118 | 3059 | pH 7.2, 37 °C | Open record: GatCAB (Glu-tRNAGln/Asp-tRNAAsn amidotransferase), L-glutamyl-tRNA(Gln) (H. pylori tRNAGln) |