Values CatLog read from this paper
Potentiometric and further kinetic characterization of the flavin-binding domain of Saccharomyces cerevisiae flavocytochrome b2. Inhibition by anions binding in the active site
PMID 17373777doi:10.1021/bi602634yOpen in Browse
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CatLog read 20 records (56 values) from this paper: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | hexacyanoferrate(III)apparent Km | — | 1 | — | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), hexacyanoferrate(III) |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | hexacyanoferrate(III) | — | — | 110 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), hexacyanoferrate(III) |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate | 259 | 0.66 | 407 | pH 7, 30 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate100 mM phosphate buffer; recombinant | 133 | 1.18 | 113 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate100 mM phosphate buffer | 117 | 0.89 | 131 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate100 mM phosphate buffer; 1 mM EDTA | 240 | 0.66 | 363 | pH 7.5, 25 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate10 mM Tris/HCl buffer; NaCl to I = 0.1 M | 214 | 0.86 | 249 | pH 7.5, 25 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate10 mM ferricyanide; recombinant | 101 | 2.8 | 36 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate100 mM phosphate buffer; + 400 mM K+ acetate; recombinant | 86 | 4.6 | 19 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate100 mM phosphate buffer; + 400 mM K+ chloride; recombinant | 60 | 2.3 | 26 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate100 mM phosphate buffer; + 400 mM K+ bromide; recombinant | 45 | 4 | 11 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate1.5 mM ferricyanide | 71 | 0.9 | 79 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer; + 400 mM K+ acetate | 113 | 2.7 | 42 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer; + 400 mM K+ chloride | 75 | 1.5 | 50 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer; + 400 mM K+ bromide | 61 | 0.94 | 65 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase)variant R289K | Saccharomyces cerevisiae | L-lactate | 9.2 | 8.7 | 1.06 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) variant R289K, L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase)variant R289K | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer; + 400 mM K+ acetate | 8.8 | 9.2 | 0.96 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) variant R289K, L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase)variant R289K | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer; + 400 mM K+ chloride | 9.2 | 6.5 | 1.41 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) variant R289K, L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase)variant R289K | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer; + 400 mM K+ bromide | 7.8 | 5.8 | 1.34 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) variant R289K, L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase)variant R289K | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer | 8.6 | 7 | 1.23 | pH 7, 5 °C | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) variant R289K, L-lactate |