EC 1.1.2.3: L-lactate dehydrogenase (cytochrome)
OxidoreductasesOpen in Browse
CatLog read 87 records for EC 1.1.2.3 from 4 papers, for 1 organism. Names in these papers: flavocytochrome b2 (L-lactate dehydrogenase), flavocytochrome b2 (L-lactate cytochrome c oxidoreductase), flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 34 records for EC 1.1.2.3 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.1.2.3 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Molecular interpretation of inhibition by excess substrate in flavocytochrome b2: a study with wild-type and Y143F mutant enzymes | Saccharomyces cerevisiae | 34 |
| Interdomain contacts in flavocytochrome b(2), a mutational analysis | Saccharomyces cerevisiae | 27 |
| Potentiometric and further kinetic characterization of the flavin-binding domain of Saccharomyces cerevisiae flavocytochrome b2. Inhibition by anions binding in the active site | Saccharomyces cerevisiae | 20 |
| Kinetic and crystallographic studies on the active site Arg289Lys mutant of flavocytochrome b2 (yeast L-lactate dehydrogenase) | Saccharomyces cerevisiae | 6 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate | 214 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant A67L | Saccharomyces cerevisiae | horse heart cytochrome c | 6 | 0.032 | 190 calc. | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant A67L, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant A67L | Saccharomyces cerevisiae | L-lactate | 185 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant A67L, L-lactate |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant A67Q | Saccharomyces cerevisiae | horse heart cytochrome c | 63 | 0.051 | 1.2 × 10³ calc. | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant A67Q, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant A67Q | Saccharomyces cerevisiae | L-lactate | 190 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant A67Q, L-lactate |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant D72A | Saccharomyces cerevisiae | horse heart cytochrome c | 171 | 0.105 | 1.63 × 10³ calc. | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant D72A, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant D72A | Saccharomyces cerevisiae | L-lactate | 225 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant D72A, L-lactate |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant E63K | Saccharomyces cerevisiae | horse heart cytochrome c | 139 | 0.119 | 1.17 × 10³ calc. | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant E63K, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant E63K | Saccharomyces cerevisiae | L-lactate | 218 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant E63K, L-lactate |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant F39A | Saccharomyces cerevisiae | horse heart cytochrome c | 39 | 0.113 | 350 calc. | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant F39A, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant F39A | Saccharomyces cerevisiae | L-lactate | 209 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant F39A, L-lactate |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant K73A | Saccharomyces cerevisiae | horse heart cytochrome c | 164 | 0.097 | 1.7 × 10³ calc. | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant K73A, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant K73A | Saccharomyces cerevisiae | L-lactate | 214 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant K73A, L-lactate |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant L65A | Saccharomyces cerevisiae | horse heart cytochrome c | 62 | 0.065 | 950 calc. | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant L65A, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant L65A | Saccharomyces cerevisiae | L-lactate | 196 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant L65A, L-lactate |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant N69K | Saccharomyces cerevisiae | horse heart cytochrome c | 108 | 0.085 | 1.3 × 10³ calc. | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant N69K, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant N69K | Saccharomyces cerevisiae | L-lactate | 208 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant N69K, L-lactate |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant P44A | Saccharomyces cerevisiae | horse heart cytochrome c | 87 | 0.02 | 4.4 × 10³ calc. | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant P44A, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant P44A | Saccharomyces cerevisiae | L-lactate | 211 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant P44A, L-lactate |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant P64Q | Saccharomyces cerevisiae | horse heart cytochrome c | 168 | 0.069 | 2.4 × 10³ calc. | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant P64Q, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant P64Q | Saccharomyces cerevisiae | L-lactate | 207 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant P64Q, L-lactate |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant P64R | Saccharomyces cerevisiae | horse heart cytochrome c | 143 | 0.061 | 2.3 × 10³ calc. | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant P64R, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant P64R | Saccharomyces cerevisiae | L-lactate | 212 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant P64R, L-lactate |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant V70M | Saccharomyces cerevisiae | horse heart cytochrome c | 184 | 0.092 | 2.0 × 10³ calc. | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant V70M, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant V70M | Saccharomyces cerevisiae | L-lactate | 218 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant V70M, L-lactate |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant Y74F | Saccharomyces cerevisiae | horse heart cytochrome c | 165 | 0.109 | 1.51 × 10³ calc. | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant Y74F, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate cytochrome c oxidoreductase)variant Y74F | Saccharomyces cerevisiae | L-lactate | 223 | — | — | pH 7, 30 °C | PMID 19821613 | Open record: flavocytochrome b2 (L-lactate cytochrome c oxidoreductase) variant Y74F, L-lactate |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | ferricyanide | — | 0.07 | — | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), ferricyanide |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | horse heart cytochrome c0.1 M phosphate; I = 0.22 M | — | 0.045 | — | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), horse heart cytochrome c |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | horse heart cytochrome c0.1 M phosphate + 500 mM acetate | — | 0.355 | — | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), horse heart cytochrome c |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | horse heart cytochrome c0.1 M phosphate; I = 0.22 M | — | 0.131 | — | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), horse heart cytochrome c |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | horse heart cytochrome c0.043 M phosphate; I = 0.1 M | — | 0.0179 | — | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), horse heart cytochrome c |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | L-[2-2H]lactate1 mM ferricyanide (94% saturation); cosubstrate ferricyanide | 25 | 0.42 | 59 | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), L-[2-2H]lactate |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | L-[2-2H]lactate450 µM horse heart cytochrome c (91% saturation) | 17 | 0.52 | 33 | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), L-[2-2H]lactate |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | L-[2-2H]lactate100 mM phosphate; pH 7 (I = 0.22 M) | 51 | 0.53 | 96 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), L-[2-2H]lactate |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | L-[2-2H]lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate cytochrome c | 47 | 0.95 | 49 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), L-[2-2H]lactate |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | L-[2-2H]lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate ferricyanide | 47 | 0.61 | 77 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), L-[2-2H]lactate |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | L-lactate1 mM ferricyanide (94% saturation); cosubstrate ferricyanide | 101 | 0.62 | 163 | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), L-lactate |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | L-lactate450 µM horse heart cytochrome c (91% saturation) | 61 | 0.24 | 254 | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), L-lactate |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | L-lactate100 mM phosphate; pH 7 (I = 0.22 M) | 155 | 0.29 | 534 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), L-lactate |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | L-lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate cytochrome c | 165 | 0.57 | 289 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), L-lactate |
| flavocytochrome b2 (L-lactate dehydrogenase) | Saccharomyces cerevisiae | L-lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate ferricyanide | 218 | 0.3 | 727 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase), L-lactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant R289K | Saccharomyces cerevisiae | horse heart cytochrome c | 16.5 | — | — | pH 7, 30 °C | PMID 10727218 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant R289K, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate dehydrogenase)variant R289K | Saccharomyces cerevisiae | horse heart cytochrome capparent Km | — | 0.074 | — | pH 7, 30 °C | PMID 10727218 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant R289K, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate dehydrogenase)variant R289K | Saccharomyces cerevisiae | L-3-phenyllactate | 6 | 1.43 | 4.2 | pH 7, 30 °C | PMID 10727218 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant R289K, L-3-phenyllactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant R289K | Saccharomyces cerevisiae | L-[2-2H]lactate | 4.7 | 4.3 | 1.1 | pH 7, 30 °C | PMID 10727218 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant R289K, L-[2-2H]lactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant R289K | Saccharomyces cerevisiae | L-lactate | 20.6 | 3.2 | 6.4 | pH 7, 30 °C | PMID 10727218 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant R289K, L-lactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant R289K | Saccharomyces cerevisiae | potassium ferricyanide | — | 0.03 | — | pH 7, 30 °C | PMID 10727218 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant R289K, potassium ferricyanide |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | ferricyanide0.1 M phosphate; I = 0.22 M | — | 0.61 | — | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, ferricyanide |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | ferricyanide0.1 M phosphate + 500 mM acetate | — | 2.5 | — | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, ferricyanide |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | ferricyanide0.1 M phosphate; I = 0.22 M | — | 1.5 | — | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, ferricyanide |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | ferricyanide0.043 M phosphate; I = 0.1 M | — | 0.85 | — | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, ferricyanide |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | horse heart cytochrome c0.1 M phosphate; I = 0.22 M | — | 0.131 | — | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | horse heart cytochrome c0.1 M phosphate + 50 mM acetate | — | 0.061 | — | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | horse heart cytochrome c0.1 M phosphate; I = 0.22 M | — | 0.121 | — | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | horse heart cytochrome c0.043 M phosphate; I = 0.1 M | — | 0.009 | — | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, horse heart cytochrome c |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | L-[2-2H]lactate | 28 | 5.6 | 5 | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, L-[2-2H]lactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | L-[2-2H]lactate100 mM phosphate; pH 7 (I = 0.22 M) | 140 | 2.5 | 55 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, L-[2-2H]lactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | L-[2-2H]lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate ferricyanide | 116 | 2.2 | 53 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, L-[2-2H]lactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | L-[2-2H]lactate100 mM phosphate; pH 7 (I = 0.22 M) | 34 | 0.66 | 51 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, L-[2-2H]lactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | L-[2-2H]lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate cytochrome c | 22 | 0.92 | 24 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, L-[2-2H]lactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | L-lactate0.1 M Na+/K+ phosphate; pH 7 (I = 0.22 M) | 120 | 3.4 | 35 | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, L-lactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | L-lactate100 mM phosphate; pH 7 (I = 0.22 M) | 382 | 1.4 | 227 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, L-lactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | L-lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate ferricyanide | 272 | 1.3 | 209 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, L-lactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | L-lactate100 mM phosphate; pH 7 (I = 0.22 M) | 61 | 0.34 | 179 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, L-lactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | L-lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate cytochrome c | 28 | 0.17 | 162 | pH 7, 30 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, L-lactate |
| flavocytochrome b2 (L-lactate dehydrogenase)variant Y143F | Saccharomyces cerevisiae | L-lactate0.1 M phosphate; pH 7 + 500 mM acetate | — | 6.4 | — | pH 7, 5 °C | PMID 9188712 | Open record: flavocytochrome b2 (L-lactate dehydrogenase) variant Y143F, L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | hexacyanoferrate(III)apparent Km | — | 1 | — | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), hexacyanoferrate(III) |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | hexacyanoferrate(III) | — | — | 110 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), hexacyanoferrate(III) |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate | 259 | 0.66 | 407 | pH 7, 30 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate100 mM phosphate buffer; recombinant | 133 | 1.18 | 113 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate100 mM phosphate buffer | 117 | 0.89 | 131 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate100 mM phosphate buffer; 1 mM EDTA | 240 | 0.66 | 363 | pH 7.5, 25 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate10 mM Tris/HCl buffer; NaCl to I = 0.1 M | 214 | 0.86 | 249 | pH 7.5, 25 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate10 mM ferricyanide; recombinant | 101 | 2.8 | 36 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate100 mM phosphate buffer; + 400 mM K+ acetate; recombinant | 86 | 4.6 | 19 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate100 mM phosphate buffer; + 400 mM K+ chloride; recombinant | 60 | 2.3 | 26 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate100 mM phosphate buffer; + 400 mM K+ bromide; recombinant | 45 | 4 | 11 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate1.5 mM ferricyanide | 71 | 0.9 | 79 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer; + 400 mM K+ acetate | 113 | 2.7 | 42 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer; + 400 mM K+ chloride | 75 | 1.5 | 50 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer; + 400 mM K+ bromide | 61 | 0.94 | 65 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase), L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase)variant R289K | Saccharomyces cerevisiae | L-lactate | 9.2 | 8.7 | 1.06 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) variant R289K, L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase)variant R289K | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer; + 400 mM K+ acetate | 8.8 | 9.2 | 0.96 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) variant R289K, L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase)variant R289K | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer; + 400 mM K+ chloride | 9.2 | 6.5 | 1.41 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) variant R289K, L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase)variant R289K | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer; + 400 mM K+ bromide | 7.8 | 5.8 | 1.34 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) variant R289K, L-lactate |
| flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase)variant R289K | Saccharomyces cerevisiae | L-lactate0.1 M phosphate buffer | 8.6 | 7 | 1.23 | pH 7, 5 °C | PMID 17373777 | Open record: flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) variant R289K, L-lactate |