CatLogenzyme kinetics catalog

Values CatLog read from this paper

Molecular interpretation of inhibition by excess substrate in flavocytochrome b2: a study with wild-type and Y143F mutant enzymes

PMID 9188712doi:10.1021/bi963035dOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 34 records (74 values) from this paper: flavocytochrome b2 (L-lactate dehydrogenase) from Saccharomyces cerevisiae.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.2.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaeferricyanide—0.07—pH 7, 5 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaehorse heart cytochrome c0.1 M phosphate; I = 0.22 M—0.045—pH 7, 5 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaehorse heart cytochrome c0.1 M phosphate + 500 mM acetate—0.355—pH 7, 5 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaehorse heart cytochrome c0.1 M phosphate; I = 0.22 M—0.131—pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaehorse heart cytochrome c0.043 M phosphate; I = 0.1 M—0.0179—pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaeL-[2-2H]lactate1 mM ferricyanide (94% saturation); cosubstrate ferricyanide250.4259pH 7, 5 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaeL-[2-2H]lactate450 µM horse heart cytochrome c (91% saturation)170.5233pH 7, 5 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaeL-[2-2H]lactate100 mM phosphate; pH 7 (I = 0.22 M)510.5396pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaeL-[2-2H]lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate cytochrome c470.9549pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaeL-[2-2H]lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate ferricyanide470.6177pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaeL-lactate1 mM ferricyanide (94% saturation); cosubstrate ferricyanide1010.62163pH 7, 5 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaeL-lactate450 µM horse heart cytochrome c (91% saturation)610.24254pH 7, 5 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaeL-lactate100 mM phosphate; pH 7 (I = 0.22 M)1550.29534pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaeL-lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate cytochrome c1650.57289pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)Saccharomyces cerevisiaeL-lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate ferricyanide2180.3727pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeferricyanide0.1 M phosphate; I = 0.22 M—0.61—pH 7, 5 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeferricyanide0.1 M phosphate + 500 mM acetate—2.5—pH 7, 5 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeferricyanide0.1 M phosphate; I = 0.22 M—1.5—pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeferricyanide0.043 M phosphate; I = 0.1 M—0.85—pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaehorse heart cytochrome c0.1 M phosphate; I = 0.22 M—0.131—pH 7, 5 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaehorse heart cytochrome c0.1 M phosphate + 50 mM acetate—0.061—pH 7, 5 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaehorse heart cytochrome c0.1 M phosphate; I = 0.22 M—0.121—pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaehorse heart cytochrome c0.043 M phosphate; I = 0.1 M—0.009—pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeL-[2-2H]lactate285.65pH 7, 5 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeL-[2-2H]lactate100 mM phosphate; pH 7 (I = 0.22 M)1402.555pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeL-[2-2H]lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate ferricyanide1162.253pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeL-[2-2H]lactate100 mM phosphate; pH 7 (I = 0.22 M)340.6651pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeL-[2-2H]lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate cytochrome c220.9224pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeL-lactate0.1 M Na+/K+ phosphate; pH 7 (I = 0.22 M)1203.435pH 7, 5 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeL-lactate100 mM phosphate; pH 7 (I = 0.22 M)3821.4227pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeL-lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate ferricyanide2721.3209pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeL-lactate100 mM phosphate; pH 7 (I = 0.22 M)610.34179pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeL-lactate43 mM phosphate; pH 7 (I = 0.1 M); cosubstrate cytochrome c280.17162pH 7, 30 °COpen
flavocytochrome b2 (L-lactate dehydrogenase)variant Y143FSaccharomyces cerevisiaeL-lactate0.1 M phosphate; pH 7 + 500 mM acetate—6.4—pH 7, 5 °COpen