Values CatLog read from this paper
Identification of acceptor substrate binding subsites +2 and +3 in the amylomaltase from Thermus thermophilus HB8
PMID 17407266doi:10.1021/bi602408jOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 19 records (29 values) from this paper: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) from Thermus thermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | gelatinized native potato starch | 0.06 | — | — | pH 6.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), gelatinized native potato starch |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | maltoheptaose | 213 | 14.9 | 14.3 calc. | pH 5.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), maltoheptaose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | maltohexaose | 304 | 17.4 | 17.5 calc. | pH 5.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), maltohexaose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | maltopentaose | 329 | 10.8 | 30.5 calc. | pH 5.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), maltopentaose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | maltotetraose | 425 | 5.4 | 79 calc. | pH 5.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), maltotetraose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | maltotriose | 317 | 2.2 | 140 calc. | pH 5.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | maltotriose | 291 | — | — | pH 6.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant D293A | Thermus thermophilus | maltotriose | 4 × 10⁻⁵ | — | — | pH 6.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant D293A, maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant D293N | Thermus thermophilus | maltotriose | 0.02 | — | — | pH 6.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant D293N, maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant D395A | Thermus thermophilus | maltotriose | 6 × 10⁻⁴ | — | — | pH 6.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant D395A, maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant D395N | Thermus thermophilus | maltotriose | 0.2 | — | — | pH 6.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant D395N, maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant E340A | Thermus thermophilus | maltotriose | 9 × 10⁻⁴ | — | — | pH 6.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant E340A, maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant E340Q | Thermus thermophilus | maltotriose | 0.006 | — | — | pH 6.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant E340Q, maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant F366L | Thermus thermophilus | gelatinized native potato starch | 0.04 | — | — | pH 6.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant F366L, gelatinized native potato starch |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant F366L | Thermus thermophilus | maltoheptaose | 159 | 15.1 | 10.5 calc. | pH 5.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant F366L, maltoheptaose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant F366L | Thermus thermophilus | maltohexaose | 188 | 15.5 | 12.1 calc. | pH 5.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant F366L, maltohexaose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant F366L | Thermus thermophilus | maltopentaose | 179 | 6.2 | 29 calc. | pH 5.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant F366L, maltopentaose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant F366L | Thermus thermophilus | maltotetraose | 106 | 2.4 | 44 calc. | pH 5.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant F366L, maltotetraose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant F366L | Thermus thermophilus | maltotriose | 111 | 3.3 | 34 calc. | pH 5.5, 70 °C | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant F366L, maltotriose |