EC 2.4.1.25: 4-alpha-glucanotransferase
TransferasesOpen in Browse
CatLog read 49 records for EC 2.4.1.25 from 5 papers, for 4 organisms. Names in these papers: amylomaltase (CgAM), amylomaltase (Tt AMase, 4-alpha-glucanotransferase), amylomaltase (PyAMase), amylomaltase (4-alpha-glucanotransferase, TBGT).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 38 records for EC 2.4.1.25 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.4.1.25 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Identification of acceptor substrate binding subsites +2 and +3 in the amylomaltase from Thermus thermophilus HB8 | Thermus thermophilus | 19 |
| Identification of crucial amino acid residues involved in large ring cyclodextrin synthesis by amylomaltase from Corynebacterium glutamicum | Corynebacterium glutamicum | 15 |
| Significance of H461 at subsite +1 in substrate binding and transglucosylation activity of amylomaltase from Corynebacterium glutamicum | Corynebacterium glutamicum | 6 |
| Amylomaltase of Pyrobaculum aerophilum IM2 produces thermoreversible starch gels | Pyrobaculum aerophilum | 5 |
| Structural and functional analysis of substrate recognition by the 250s loop in amylomaltase from Thermus brockianus | Thermus brockianus | 4 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| amylomaltase (4-alpha-glucanotransferase, TBGT) | Thermus brockianus | maltotriose | 0.015 | 2 | 0.007 | pH 6, 70 °C | PMID 21117235 | Open record: amylomaltase (4-alpha-glucanotransferase, TBGT), maltotriose |
| amylomaltase (4-alpha-glucanotransferase, TBGT)variant F251G | Thermus brockianus | maltotriose | 0.0017 | 5.8 | 3.5 × 10⁻⁴ | pH 6, 70 °C | PMID 21117235 | Open record: amylomaltase (4-alpha-glucanotransferase, TBGT) variant F251G, maltotriose |
| amylomaltase (4-alpha-glucanotransferase, TBGT)variant Q256G | Thermus brockianus | maltotriose | 0.08 | 165 | 4.8 × 10⁻⁴ | pH 6, 70 °C | PMID 21117235 | Open record: amylomaltase (4-alpha-glucanotransferase, TBGT) variant Q256G, maltotriose |
| amylomaltase (4-alpha-glucanotransferase, TBGT)variant W258G | Thermus brockianus | maltotriose | 0.005 | 9.5 | 4.8 × 10⁻⁴ | pH 6, 70 °C | PMID 21117235 | Open record: amylomaltase (4-alpha-glucanotransferase, TBGT) variant W258G, maltotriose |
| amylomaltase (CgAM) | Corynebacterium glutamicum | glucose | 0.00728 | 0.0822 | 0.0957 | pH 6, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM), glucose |
| amylomaltase (CgAM) | Corynebacterium glutamicum | maltotriose | 3.03 × 10³ | 16.9 | 180 | PMID 29885290 | Open record: amylomaltase (CgAM), maltotriose | |
| amylomaltase (CgAM) | Corynebacterium glutamicum | maltotriose | 103 | 18.1 | 5.7 | pH 6, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM), maltotriose |
| amylomaltase (CgAM) | Corynebacterium glutamicum | pea starch | 0.002773 | — | — | pH 6, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM), pea starch |
| amylomaltase (CgAM)variant E231Y | Corynebacterium glutamicum | glucose | 0.01793 | 0.186 | 0.0957 | pH 6.5, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM) variant E231Y, glucose |
| amylomaltase (CgAM)variant E231Y | Corynebacterium glutamicum | maltotriose | 111.3 | 15.4 | 7.07 | pH 6.5, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM) variant E231Y, maltotriose |
| amylomaltase (CgAM)variant E231Y | Corynebacterium glutamicum | pea starch | 0.002792 | — | — | pH 6.5, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM) variant E231Y, pea starch |
| amylomaltase (CgAM)variant G417F | Corynebacterium glutamicum | glucose | 0.00545 | 0.0718 | 0.0773 | pH 6.5, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM) variant G417F, glucose |
| amylomaltase (CgAM)variant G417F | Corynebacterium glutamicum | maltotriose | 139.5 | 22.9 | 6.15 | pH 6.5, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM) variant G417F, maltotriose |
| amylomaltase (CgAM)variant G417F | Corynebacterium glutamicum | pea starch | 7.55 × 10⁻⁴ | — | — | pH 6.5, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM) variant G417F, pea starch |
| amylomaltase (CgAM)variant H461A | Corynebacterium glutamicum | maltotriose | 121.2 | 35.7 | 3.5 | PMID 29885290 | Open record: amylomaltase (CgAM) variant H461A, maltotriose | |
| amylomaltase (CgAM)variant H461D | Corynebacterium glutamicum | maltotriose | 143.7 | 37.2 | 3.7 | PMID 29885290 | Open record: amylomaltase (CgAM) variant H461D, maltotriose | |
| amylomaltase (CgAM)variant H461R | Corynebacterium glutamicum | maltotriose | 122 | 37.4 | 3.2 | PMID 29885290 | Open record: amylomaltase (CgAM) variant H461R, maltotriose | |
| amylomaltase (CgAM)variant H461S | Corynebacterium glutamicum | maltotriose | 136 | 35.1 | 3.8 | PMID 29885290 | Open record: amylomaltase (CgAM) variant H461S, maltotriose | |
| amylomaltase (CgAM)variant H461W | Corynebacterium glutamicum | maltotriose | 120.8 | 36.2 | 3.3 | PMID 29885290 | Open record: amylomaltase (CgAM) variant H461W, maltotriose | |
| amylomaltase (CgAM)variant P228Y | Corynebacterium glutamicum | glucose | 0.00728 | 0.0741 | 0.0987 | pH 6.5, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM) variant P228Y, glucose |
| amylomaltase (CgAM)variant P228Y | Corynebacterium glutamicum | maltotriose | 43.8 | 19.7 | 2.32 | pH 6.5, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM) variant P228Y, maltotriose |
| amylomaltase (CgAM)variant P228Y | Corynebacterium glutamicum | pea starch | 0.00228 | — | — | pH 6.5, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM) variant P228Y, pea starch |
| amylomaltase (CgAM)variant Y23A | Corynebacterium glutamicum | glucose | 0.00625 | 0.14 | 0.0448 | pH 6.5, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM) variant Y23A, glucose |
| amylomaltase (CgAM)variant Y23A | Corynebacterium glutamicum | maltotriose | 110.8 | 12.27 | 9.05 | pH 6.5, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM) variant Y23A, maltotriose |
| amylomaltase (CgAM)variant Y23A | Corynebacterium glutamicum | pea starch | 0.001572 | — | — | pH 6.5, 30 °C | PMID 36698977 | Open record: amylomaltase (CgAM) variant Y23A, pea starch |
| amylomaltase (PyAMase) | Pyrobaculum aerophilum | maltoheptaose | 8.7 | 1.5 | 5.8 | pH 6.7, 80 °C | PMID 16151092 | Open record: amylomaltase (PyAMase), maltoheptaose |
| amylomaltase (PyAMase) | Pyrobaculum aerophilum | maltohexaose | 8.9 | 3.2 | 2.8 | pH 6.7, 80 °C | PMID 16151092 | Open record: amylomaltase (PyAMase), maltohexaose |
| amylomaltase (PyAMase) | Pyrobaculum aerophilum | maltopentaose | 24.8 | 6.9 | 3.6 | pH 6.7, 80 °C | PMID 16151092 | Open record: amylomaltase (PyAMase), maltopentaose |
| amylomaltase (PyAMase) | Pyrobaculum aerophilum | maltotetraose | 51.2 | 2.1 | 24 | pH 6.7, 80 °C | PMID 16151092 | Open record: amylomaltase (PyAMase), maltotetraose |
| amylomaltase (PyAMase) | Pyrobaculum aerophilum | maltotriose | 115 | 3.7 | 31 | pH 6.7, 80 °C | PMID 16151092 | Open record: amylomaltase (PyAMase), maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | gelatinized native potato starch | 0.06 | — | — | pH 6.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), gelatinized native potato starch |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | maltoheptaose | 213 | 14.9 | 14.3 calc. | pH 5.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), maltoheptaose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | maltohexaose | 304 | 17.4 | 17.5 calc. | pH 5.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), maltohexaose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | maltopentaose | 329 | 10.8 | 30.5 calc. | pH 5.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), maltopentaose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | maltotetraose | 425 | 5.4 | 79 calc. | pH 5.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), maltotetraose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | maltotriose | 317 | 2.2 | 140 calc. | pH 5.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase) | Thermus thermophilus | maltotriose | 291 | — | — | pH 6.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase), maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant D293A | Thermus thermophilus | maltotriose | 4 × 10⁻⁵ | — | — | pH 6.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant D293A, maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant D293N | Thermus thermophilus | maltotriose | 0.02 | — | — | pH 6.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant D293N, maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant D395A | Thermus thermophilus | maltotriose | 6 × 10⁻⁴ | — | — | pH 6.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant D395A, maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant D395N | Thermus thermophilus | maltotriose | 0.2 | — | — | pH 6.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant D395N, maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant E340A | Thermus thermophilus | maltotriose | 9 × 10⁻⁴ | — | — | pH 6.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant E340A, maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant E340Q | Thermus thermophilus | maltotriose | 0.006 | — | — | pH 6.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant E340Q, maltotriose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant F366L | Thermus thermophilus | gelatinized native potato starch | 0.04 | — | — | pH 6.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant F366L, gelatinized native potato starch |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant F366L | Thermus thermophilus | maltoheptaose | 159 | 15.1 | 10.5 calc. | pH 5.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant F366L, maltoheptaose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant F366L | Thermus thermophilus | maltohexaose | 188 | 15.5 | 12.1 calc. | pH 5.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant F366L, maltohexaose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant F366L | Thermus thermophilus | maltopentaose | 179 | 6.2 | 29 calc. | pH 5.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant F366L, maltopentaose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant F366L | Thermus thermophilus | maltotetraose | 106 | 2.4 | 44 calc. | pH 5.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant F366L, maltotetraose |
| amylomaltase (Tt AMase, 4-alpha-glucanotransferase)variant F366L | Thermus thermophilus | maltotriose | 111 | 3.3 | 34 calc. | pH 5.5, 70 °C | PMID 17407266 | Open record: amylomaltase (Tt AMase, 4-alpha-glucanotransferase) variant F366L, maltotriose |