CatLogenzyme kinetics catalog

Values CatLog read from this paper

Rat NAD+-dependent 3alpha-hydroxysteroid dehydrogenase (AKR1C17): a member of the aldo-keto reductase family highly expressed in kidney cytosol

PMID 17475203doi:10.1016/j.abb.2007.04.003Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 51 records (126 values) from this paper: AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd) from Rattus norvegicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 11 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.213

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus4-Androsten-3α-ol-17-one0.183 × 10⁻⁴600pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus4-Androstene-3α,17β-diol0.1270.001967pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus4-Pregnen-3α-ol-20-one0.1337 × 10⁻⁴180pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus5α-Androstan-3α-ol-17-one0.01230.00393.2pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus5α-Androstane-3α,17β-diol0.00350.00381pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus5α-Pregnan-3α-ol-20-one0.00180.00121.5pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus5α-Pregnane-3α,20α-diol0.00380.00192pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus5β-Androstan-3α-ol-17-one0.0287 × 10⁻⁴40pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus5β-androstane-3,17-dione0.0470.002122 calc.pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus5β-Androstane-3α,17β-diol0.01320.00113.2pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus5β-pregnan-20α-ol-3-one0.1150.002743 calc.pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus5β-Pregnan-3α-ol-20-one0.083 × 10⁻⁴270pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus5β-pregnan-3α-ol-20-oneTHP varied with 1 mM NAD+; cosubstrate NAD+—3 × 10⁻⁴—pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus5β-pregnan-3α-ol-20-oneTHP varied with 1 mM NADP+; cosubstrate NADP+—0.0025—pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus5β-pregnane-3,20-dione0.1050.002444 calc.pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus5β-Pregnane-3α,20α-diol0.1325 × 10⁻⁴270pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus6-tert-butyl-2,3-epoxy-5-cyclohexene-1,4-dione0.10.0234.3 calc.pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicus9,10-phenanthrenequinone0.180.0384.8 calc.pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusChenodeoxycholic acid0.0680.001257pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusCholic acid0.0380.0152.5pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicuscis-benzene dihydrodiol0.0432.50.017 calc.pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusdehydrolithocholic acid0.0224 × 10⁻⁴54 calc.pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusDeoxycholic acid0.01450.001410.3pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusdiacetyl0.170.420.40 calc.pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusGlycochenodeoxycholic acid0.1020.001102pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusGlycolithocholic acid0.0632 × 10⁻⁴320pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusGlycoursodeoxycholic acid0.1180.001392pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusHyodeoxycholic acid0.0020.00171.2pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusisatin0.280.02213 calc.pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusLithocholic acid0.0323 × 10⁻⁴105pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusMurocholic acid0.00350.00341pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusNAD+0.0650.080.82pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusNADP+0.0421.10.038pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusS-tetralol0.0171.70.0098 calc.pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusTaurolithocholic acid0.0853 × 10⁻⁴280pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusTauroursodeoxycholic acid0.1320.001132pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicustrans-benzene dihydrodiol0.00980.790.012 calc.pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusUrsodeoxycholic acid0.0853 × 10⁻⁴280pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)Rattus norvegicusβ-Muricholic acid0.00970.00581.7pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)variant E276RRattus norvegicus5β-pregnan-3α-ol-20-oneTHP varied with 1 mM NAD+; cosubstrate NAD+—0.0028—pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)variant E276RRattus norvegicus5β-pregnan-3α-ol-20-oneTHP varied with 1 mM NADP+; cosubstrate NADP+—4 × 10⁻⁴—pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)variant E276RRattus norvegicusNAD+0.0471.10.042pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)variant E276RRattus norvegicusNADP+0.0580.0222.65pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)variant Q270KRattus norvegicus5β-pregnan-3α-ol-20-oneTHP varied with 1 mM NAD+; cosubstrate NAD+—0.0028—pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)variant Q270KRattus norvegicus5β-pregnan-3α-ol-20-oneTHP varied with 1 mM NADP+; cosubstrate NADP+—5 × 10⁻⁴—pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)variant Q270KRattus norvegicusNAD+0.070.30.23pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)variant Q270KRattus norvegicusNADP+0.0520.20.27pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)variant Q270K/E276RRattus norvegicus5β-pregnan-3α-ol-20-oneTHP varied with 1 mM NAD+; cosubstrate NAD+—0.001—pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)variant Q270K/E276RRattus norvegicus5β-pregnan-3α-ol-20-oneTHP varied with 1 mM NADP+; cosubstrate NADP+—1 × 10⁻⁴—pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)variant Q270K/E276RRattus norvegicusNAD+0.1081.10.098pH 7.4, 25 °COpen
AKR1C17 (NAD+-dependent 3α-hydroxysteroid dehydrogenase, RAKd)variant Q270K/E276RRattus norvegicusNADP+0.020.0036.7pH 7.4, 25 °COpen