Values CatLog read from this paper
Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase
PMID 17542990doi:10.1111/j.1742-4658.2007.05870.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (60 values) from this paper: CMP kinase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CMP kinasevariant D132A | Escherichia coli | 2'-deoxycytidine 5'-monophosphate | 0.73 | 1.8 | 0.4 | pH 7.4, 30 °C | Open record: CMP kinase variant D132A, 2'-deoxycytidine 5'-monophosphate |
| CMP kinasevariant D132A | Escherichia coli | cytidine 5'-monophosphate | 4.1 | 2.9 | 1.41 | pH 7.4, 30 °C | Open record: CMP kinase variant D132A, cytidine 5'-monophosphate |
| CMP kinasevariant D132A | Escherichia coli | uridine 5'-monophosphate | 9.9 | 7.9 | 1.25 | pH 7.4, 30 °C | Open record: CMP kinase variant D132A, uridine 5'-monophosphate |
| CMP kinasevariant D132H | Escherichia coli | 2'-deoxycytidine 5'-monophosphate | 0.06 | 0.055 | 1.1 | pH 7.4, 30 °C | Open record: CMP kinase variant D132H, 2'-deoxycytidine 5'-monophosphate |
| CMP kinasevariant D132H | Escherichia coli | cytidine 5'-monophosphate | 0.069 | 1.3 | 0.053 | pH 7.4, 30 °C | Open record: CMP kinase variant D132H, cytidine 5'-monophosphate |
| CMP kinasevariant D132H | Escherichia coli | uridine 5'-monophosphate | 0.013 | 3.9 | 0.0033 | pH 7.4, 30 °C | Open record: CMP kinase variant D132H, uridine 5'-monophosphate |
| CMP kinasevariant D132N | Escherichia coli | 2'-deoxycytidine 5'-monophosphate | 0.15 | 0.08 | 1.88 | pH 7.4, 30 °C | Open record: CMP kinase variant D132N, 2'-deoxycytidine 5'-monophosphate |
| CMP kinasevariant D132N | Escherichia coli | cytidine 5'-monophosphate | 1.4 | 2.6 | 0.54 | pH 7.4, 30 °C | Open record: CMP kinase variant D132N, cytidine 5'-monophosphate |
| CMP kinasevariant D132N | Escherichia coli | uridine 5'-monophosphate | 0.45 | 5.4 | 0.083 | pH 7.4, 30 °C | Open record: CMP kinase variant D132N, uridine 5'-monophosphate |
| CMP kinasevariant D132S | Escherichia coli | 2'-deoxycytidine 5'-monophosphate | 21.1 | 0.09 | 230 | pH 7.4, 30 °C | Open record: CMP kinase variant D132S, 2'-deoxycytidine 5'-monophosphate |
| CMP kinasevariant D132S | Escherichia coli | cytidine 5'-monophosphate | 22.4 | 0.038 | 589 | pH 7.4, 30 °C | Open record: CMP kinase variant D132S, cytidine 5'-monophosphate |
| CMP kinasevariant D132S | Escherichia coli | uridine 5'-monophosphate | 8.3 | 8 | 1.04 | pH 7.4, 30 °C | Open record: CMP kinase variant D132S, uridine 5'-monophosphate |
| CMP kinasevariant R110M | Escherichia coli | 2'-deoxycytidine 5'-monophosphate | 0.05 | 7.3 | 0.0068 | pH 7.4, 30 °C | Open record: CMP kinase variant R110M, 2'-deoxycytidine 5'-monophosphate |
| CMP kinasevariant R110M | Escherichia coli | cytidine 5'-monophosphate | 0.23 | 20.2 | 0.0114 | pH 7.4, 30 °C | Open record: CMP kinase variant R110M, cytidine 5'-monophosphate |
| CMP kinasevariant R110M | Escherichia coli | uridine 5'-monophosphate | 0.054 | 11.3 | 0.0048 | pH 7.4, 30 °C | Open record: CMP kinase variant R110M, uridine 5'-monophosphate |
| CMP kinasevariant R188M | Escherichia coli | 2'-deoxycytidine 5'-monophosphate | 0.04 | 0.77 | 0.052 | pH 7.4, 30 °C | Open record: CMP kinase variant R188M, 2'-deoxycytidine 5'-monophosphate |
| CMP kinasevariant R188M | Escherichia coli | cytidine 5'-monophosphate | 0.12 | 1 | 0.12 | pH 7.4, 30 °C | Open record: CMP kinase variant R188M, cytidine 5'-monophosphate |
| CMP kinasevariant S36A | Escherichia coli | 2'-deoxycytidine 5'-monophosphate | 14.5 | 3.5 | 4.1 | pH 7.4, 30 °C | Open record: CMP kinase variant S36A, 2'-deoxycytidine 5'-monophosphate |
| CMP kinasevariant S36A | Escherichia coli | cytidine 5'-monophosphate | 63 | 2.5 | 25.2 | pH 7.4, 30 °C | Open record: CMP kinase variant S36A, cytidine 5'-monophosphate |
| CMP kinasevariant S36A | Escherichia coli | uridine 5'-monophosphate | 0.57 | 1.9 | 0.3 | pH 7.4, 30 °C | Open record: CMP kinase variant S36A, uridine 5'-monophosphate |