Values CatLog read from this paper
Delta 5-3beta-hydroxysteroid dehydrogenase (3 beta HSD) from Digitalis lanata. Heterologous expression and characterisation of the recombinant enzyme
PMID 17564944doi:10.1055/s-2007-981537Open in Browse
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CatLog read 8 records (8 values) from this paper: Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD) from Digitalis lanata.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD) | Digitalis lanata | 5-Androsten-3β-ol-17-one | — | 0.642 | — | pH 7.8, 50 °C | Open record: Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD), 5-Androsten-3β-ol-17-one |
| Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD) | Digitalis lanata | 5-pregnen-3β-ol-20-one | — | 0.09 | — | pH 7.8, 50 °C | Open record: Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD), 5-pregnen-3β-ol-20-one |
| Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD) | Digitalis lanata | 5α-Pregnan-3β-ol-20-one | — | 0.116 | — | pH 7.8, 50 °C | Open record: Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD), 5α-Pregnan-3β-ol-20-one |
| Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD) | Digitalis lanata | 5α-Pregnane-3,20-dione | — | 0.106 | — | pH 7.8, 50 °C | Open record: Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD), 5α-Pregnane-3,20-dione |
| Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD) | Digitalis lanata | 5β-Pregnan-3β-ol-20-one | — | 0.314 | — | pH 7.8, 50 °C | Open record: Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD), 5β-Pregnan-3β-ol-20-one |
| Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD) | Digitalis lanata | 5β-Pregnane-3,20-dione | — | 1.725 | — | pH 7.8, 50 °C | Open record: Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD), 5β-Pregnane-3,20-dione |
| Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD) | Digitalis lanata | NAD+ | — | 0.26 | — | pH 7.8, 50 °C | Open record: Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD), NAD+ |
| Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD) | Digitalis lanata | NADH | — | 0.642 | — | pH 7.8, 50 °C | Open record: Δ5-3β-hydroxysteroid dehydrogenase (3βHSD; recombinant pQ3βHSD), NADH |