Values CatLog read from this paper
Analysis of the putative substrate binding region of hyperthermophilic endoglucanase from Pyrococcus horikoshii
PMID 17928686doi:10.1271/bbb.70322Open in Browse
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CatLog read 11 records (33 values) from this paper: hyperthermophilic endoglucanase EGPh (family 5 cellulase) from Pyrococcus horikoshii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| hyperthermophilic endoglucanase EGPh (family 5 cellulase) | Pyrococcus horikoshii | 4-nitrophenyl beta-D-cellobioside | 0.157 | 1.12 | 0.14 | pH 5.5, 50 °C | Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase), 4-nitrophenyl beta-D-cellobioside |
| hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant C159A | Pyrococcus horikoshii | 4-nitrophenyl beta-D-cellobioside | 0.144 | 1.9 | 0.076 | pH 5.5, 50 °C | Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant C159A, 4-nitrophenyl beta-D-cellobioside |
| hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant E163A | Pyrococcus horikoshii | 4-nitrophenyl beta-D-cellobioside | 0.036 | 1.55 | 0.023 | pH 5.5, 50 °C | Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant E163A, 4-nitrophenyl beta-D-cellobioside |
| hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant G158A | Pyrococcus horikoshii | 4-nitrophenyl beta-D-cellobioside | 0.092 | 1.37 | 0.068 | pH 5.5, 50 °C | Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant G158A, 4-nitrophenyl beta-D-cellobioside |
| hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant H155A | Pyrococcus horikoshii | 4-nitrophenyl beta-D-cellobioside | 0.006 | 6.69 | 0.001 | pH 5.5, 50 °C | Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant H155A, 4-nitrophenyl beta-D-cellobioside |
| hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant H161A | Pyrococcus horikoshii | 4-nitrophenyl beta-D-cellobioside | 0.167 | 1.23 | 0.136 | pH 5.5, 50 °C | Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant H161A, 4-nitrophenyl beta-D-cellobioside |
| hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant I157A | Pyrococcus horikoshii | 4-nitrophenyl beta-D-cellobioside | 0.115 | 0.88 | 0.13 | pH 5.5, 50 °C | Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant I157A, 4-nitrophenyl beta-D-cellobioside |
| hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant I162A | Pyrococcus horikoshii | 4-nitrophenyl beta-D-cellobioside | 0.003 | 4.57 | 0.001 | pH 5.5, 50 °C | Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant I162A, 4-nitrophenyl beta-D-cellobioside |
| hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant P164A | Pyrococcus horikoshii | 4-nitrophenyl beta-D-cellobioside | 0.037 | 1.58 | 0.024 | pH 5.5, 50 °C | Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant P164A, 4-nitrophenyl beta-D-cellobioside |
| hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant R156A | Pyrococcus horikoshii | 4-nitrophenyl beta-D-cellobioside | 0.083 | 5.85 | 0.014 | pH 5.5, 50 °C | Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant R156A, 4-nitrophenyl beta-D-cellobioside |
| hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant T160A | Pyrococcus horikoshii | 4-nitrophenyl beta-D-cellobioside | 0.129 | 0.92 | 0.141 | pH 5.5, 50 °C | Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant T160A, 4-nitrophenyl beta-D-cellobioside |