EC 3.2.1.4: cellulase

HydrolasesOpen in Browse

CatLog read 72 records for EC 3.2.1.4 from 8 papers, for 7 organisms. Names in these papers: β-glucanase Umcel9y-1 (GH9 endoglucanase), CtLic26A (family GH26 β-1,3:1,4 glucanase), hyperthermophilic endoglucanase EGPh (family 5 cellulase), endoglucanase K (EGK) and 4 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 130 records for EC 3.2.1.4 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.4 in Browse.

Papers

Papers with records for EC 3.2.1.4
PaperOrganismsRecords
A novel efficient β-glucanase from a paddy soil microbial metagenome with versatile activities—23
Probing the beta-1,3:1,4 glucanase, CtLic26A, with a thio-oligosaccharide and enzyme variantsClostridium thermocellum20
Analysis of the putative substrate binding region of hyperthermophilic endoglucanase from Pyrococcus horikoshiiPyrococcus horikoshii11
Site-directed mutagenesis of the putative active site of endoglucanase K from Bacillus sp. KSM-330Bacillus sp. KSM-3306
An endoglucanase, EglA, from the hyperthermophilic archaeon Pyrococcus furiosus hydrolyzes beta-1,4 bonds in mixed-linkage (1-->3),(1-->4)-beta-D-glucans and cellulosePyrococcus furiosus5
Modulating the thermostability of Endoglucanase I from Trichoderma reesei using computational approachesTrichoderma reesei4
Characterization of a novel bifunctional endo-β-1,4-glucanase/mannanase from Aspergillus oryzaeAspergillus oryzae2
Harnessing the potential of chloroplast-derived expression elements for enhanced production of cellulases in Escherichia coliThermotoga maritima1

Records

Records for EC 3.2.1.4 read from the paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaperOpen record
CtLic26A (family GH26 β-1,3:1,4 glucanase)Clostridium thermocellumGlc-β1,4-Glc-β1,3-Glc-methylumbelliferyl0.380.113.42PMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase), Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl
CtLic26A (family GH26 β-1,3:1,4 glucanase)Clostridium thermocellumhigh viscosity β-glucan (Megazyme)1.23 × 10³——pH 7, 60 °CPMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase), high viscosity β-glucan (Megazyme)
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant E258AClostridium thermocellumGlc-β1,4-Glc-β1,3-Glc-methylumbelliferyl0.0270.1880.138PMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant E258A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant E258AClostridium thermocellumhigh viscosity β-glucan (Megazyme)430——pH 7, 60 °CPMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant E258A, high viscosity β-glucan (Megazyme)
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant E70AClostridium thermocellumGlc-β1,4-Glc-β1,3-Glc-methylumbelliferyl8.3 × 10⁻⁵0.1550.0053PMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant E70A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant E70AClostridium thermocellumhigh viscosity β-glucan (Megazyme)135——pH 7, 60 °CPMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant E70A, high viscosity β-glucan (Megazyme)
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant F41AClostridium thermocellumGlc-β1,4-Glc-β1,3-Glc-methylumbelliferyl1.7 × 10⁻⁵0.1391.7 × 10⁻⁵PMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant F41A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant F41AClostridium thermocellumhigh viscosity β-glucan (Megazyme)27——pH 7, 60 °CPMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant F41A, high viscosity β-glucan (Megazyme)
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant K260AClostridium thermocellumGlc-β1,4-Glc-β1,3-Glc-methylumbelliferyl0.0531.1620.047PMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant K260A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant K260AClostridium thermocellumhigh viscosity β-glucan (Megazyme)83——pH 7, 60 °CPMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant K260A, high viscosity β-glucan (Megazyme)
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant N157AClostridium thermocellumGlc-β1,4-Glc-β1,3-Glc-methylumbelliferyl0.01220.0111.07PMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant N157A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant N157AClostridium thermocellumhigh viscosity β-glucan (Megazyme)1.3 × 10³——pH 7, 60 °CPMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant N157A, high viscosity β-glucan (Megazyme)
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant Q18AClostridium thermocellumGlc-β1,4-Glc-β1,3-Glc-methylumbelliferyl0.0351.7630.02PMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant Q18A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant Q18AClostridium thermocellumhigh viscosity β-glucan (Megazyme)380——pH 7, 60 °CPMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant Q18A, high viscosity β-glucan (Megazyme)
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant W114AClostridium thermocellumGlc-β1,4-Glc-β1,3-Glc-methylumbelliferyl——0.0027PMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant W114A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant W114AClostridium thermocellumhigh viscosity β-glucan (Megazyme)1.42 × 10³——pH 7, 60 °CPMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant W114A, high viscosity β-glucan (Megazyme)
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant W72AClostridium thermocellumGlc-β1,4-Glc-β1,3-Glc-methylumbelliferyl——0.011PMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant W72A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant W72AClostridium thermocellumhigh viscosity β-glucan (Megazyme)2.25 × 10³——pH 7, 60 °CPMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant W72A, high viscosity β-glucan (Megazyme)
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant Y115AClostridium thermocellumGlc-β1,4-Glc-β1,3-Glc-methylumbelliferyl8.3 × 10⁻⁴0.260.0028PMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant Y115A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl
CtLic26A (family GH26 β-1,3:1,4 glucanase)variant Y115AClostridium thermocellumhigh viscosity β-glucan (Megazyme)250——pH 7, 60 °CPMID 18292875Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant Y115A, high viscosity β-glucan (Megazyme)
EgmA (bifunctional endo-β-1,4-glucanase/mannanase)Aspergillus oryzaecarob galactomannan (Megazyme)7.9——pH 4, 50 °CPMID 42098350Open record: EgmA (bifunctional endo-β-1,4-glucanase/mannanase), carob galactomannan (Megazyme)
EgmA (bifunctional endo-β-1,4-glucanase/mannanase)Aspergillus oryzaekonjac glucomannan (Megazyme)115.5——pH 4, 50 °CPMID 42098350Open record: EgmA (bifunctional endo-β-1,4-glucanase/mannanase), konjac glucomannan (Megazyme)
endoglucanaseThermotoga maritimacarboxymethyl cellulose8.05——pH 4, 90 °CPMID 39902317Open record: endoglucanase, carboxymethyl cellulose
endoglucanase EglAPyrococcus furiosusp-nitrophenyl β-D-cellobioside0.790.158.7pH 6, 95 °CPMID 9864341Open record: endoglucanase EglA, p-nitrophenyl β-D-cellobioside
endoglucanase EglAPyrococcus furiosusp-nitrophenyl β-D-cellopentaoside1.40.366.5pH 6, 95 °CPMID 9864341Open record: endoglucanase EglA, p-nitrophenyl β-D-cellopentaoside
endoglucanase EglAPyrococcus furiosusp-nitrophenyl β-D-cellotetraoside3.60.1249pH 6, 95 °CPMID 9864341Open record: endoglucanase EglA, p-nitrophenyl β-D-cellotetraoside
endoglucanase EglAPyrococcus furiosusp-nitrophenyl β-D-cellotrioside8.30.061220pH 6, 95 °CPMID 9864341Open record: endoglucanase EglA, p-nitrophenyl β-D-cellotrioside
endoglucanase EglAPyrococcus furiosusp-nitrophenyl β-D-glucopyranoside——0.06pH 6, 95 °CPMID 9864341Open record: endoglucanase EglA, p-nitrophenyl β-D-glucopyranoside
Endoglucanase I (EGI, Cel7B)Trichoderma reesei4-methylumbelliferyl beta-D-cellobioside0.010.1250.08pH 5, 45 °CPMID 25784767Open record: Endoglucanase I (EGI, Cel7B), 4-methylumbelliferyl beta-D-cellobioside
Endoglucanase I (EGI, Cel7B)variant K272FTrichoderma reesei4-methylumbelliferyl beta-D-cellobioside0.0010.1110.01pH 5, 45 °CPMID 25784767Open record: Endoglucanase I (EGI, Cel7B) variant K272F, 4-methylumbelliferyl beta-D-cellobioside
Endoglucanase I (EGI, Cel7B)variant Q126FTrichoderma reesei4-methylumbelliferyl beta-D-cellobioside0.0030.1140.02pH 5, 45 °CPMID 25784767Open record: Endoglucanase I (EGI, Cel7B) variant Q126F, 4-methylumbelliferyl beta-D-cellobioside
Endoglucanase I (EGI, Cel7B)variant Q274VTrichoderma reesei4-methylumbelliferyl beta-D-cellobioside0.0010.10.01pH 5, 45 °CPMID 25784767Open record: Endoglucanase I (EGI, Cel7B) variant Q274V, 4-methylumbelliferyl beta-D-cellobioside
endoglucanase K (EGK)Bacillus sp. KSM-330p-nitrophenyl-beta-D-cellotrioside0.0221.50.014 calc.pH 5.2, 40 °CPMID 8075149Open record: endoglucanase K (EGK), p-nitrophenyl-beta-D-cellotrioside
endoglucanase K (EGK)variant D154NBacillus sp. KSM-330p-nitrophenyl-beta-D-cellotrioside0.0221.50.014 calc.pH 5.2, 40 °CPMID 8075149Open record: endoglucanase K (EGK) variant D154N, p-nitrophenyl-beta-D-cellotrioside
endoglucanase K (EGK)variant D191NBacillus sp. KSM-330p-nitrophenyl-beta-D-cellotrioside1.7 × 10⁻⁵2.17.9 × 10⁻⁶ calc.pH 5.2, 40 °CPMID 8075149Open record: endoglucanase K (EGK) variant D191N, p-nitrophenyl-beta-D-cellotrioside
endoglucanase K (EGK)variant D193NBacillus sp. KSM-330p-nitrophenyl-beta-D-cellotrioside5 × 10⁻⁴2.12.4 × 10⁻⁴ calc.pH 5.2, 40 °CPMID 8075149Open record: endoglucanase K (EGK) variant D193N, p-nitrophenyl-beta-D-cellotrioside
endoglucanase K (EGK)variant D300NBacillus sp. KSM-330p-nitrophenyl-beta-D-cellotrioside0.001225.8 × 10⁻⁴ calc.pH 5.2, 40 °CPMID 8075149Open record: endoglucanase K (EGK) variant D300N, p-nitrophenyl-beta-D-cellotrioside
endoglucanase K (EGK)variant E130QBacillus sp. KSM-330p-nitrophenyl-beta-D-cellotrioside3.3 × 10⁻⁵1.72.0 × 10⁻⁵ calc.pH 5.2, 40 °CPMID 8075149Open record: endoglucanase K (EGK) variant E130Q, p-nitrophenyl-beta-D-cellotrioside
hyperthermophilic endoglucanase EGPh (family 5 cellulase)Pyrococcus horikoshii4-nitrophenyl beta-D-cellobioside0.1571.120.14pH 5.5, 50 °CPMID 17928686Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase), 4-nitrophenyl beta-D-cellobioside
hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant C159APyrococcus horikoshii4-nitrophenyl beta-D-cellobioside0.1441.90.076pH 5.5, 50 °CPMID 17928686Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant C159A, 4-nitrophenyl beta-D-cellobioside
hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant E163APyrococcus horikoshii4-nitrophenyl beta-D-cellobioside0.0361.550.023pH 5.5, 50 °CPMID 17928686Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant E163A, 4-nitrophenyl beta-D-cellobioside
hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant G158APyrococcus horikoshii4-nitrophenyl beta-D-cellobioside0.0921.370.068pH 5.5, 50 °CPMID 17928686Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant G158A, 4-nitrophenyl beta-D-cellobioside
hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant H155APyrococcus horikoshii4-nitrophenyl beta-D-cellobioside0.0066.690.001pH 5.5, 50 °CPMID 17928686Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant H155A, 4-nitrophenyl beta-D-cellobioside
hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant H161APyrococcus horikoshii4-nitrophenyl beta-D-cellobioside0.1671.230.136pH 5.5, 50 °CPMID 17928686Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant H161A, 4-nitrophenyl beta-D-cellobioside
hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant I157APyrococcus horikoshii4-nitrophenyl beta-D-cellobioside0.1150.880.13pH 5.5, 50 °CPMID 17928686Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant I157A, 4-nitrophenyl beta-D-cellobioside
hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant I162APyrococcus horikoshii4-nitrophenyl beta-D-cellobioside0.0034.570.001pH 5.5, 50 °CPMID 17928686Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant I162A, 4-nitrophenyl beta-D-cellobioside
hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant P164APyrococcus horikoshii4-nitrophenyl beta-D-cellobioside0.0371.580.024pH 5.5, 50 °CPMID 17928686Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant P164A, 4-nitrophenyl beta-D-cellobioside
hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant R156APyrococcus horikoshii4-nitrophenyl beta-D-cellobioside0.0835.850.014pH 5.5, 50 °CPMID 17928686Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant R156A, 4-nitrophenyl beta-D-cellobioside
hyperthermophilic endoglucanase EGPh (family 5 cellulase)variant T160APyrococcus horikoshii4-nitrophenyl beta-D-cellobioside0.1290.920.141pH 5.5, 50 °CPMID 17928686Open record: hyperthermophilic endoglucanase EGPh (family 5 cellulase) variant T160A, 4-nitrophenyl beta-D-cellobioside
β-glucanase Umcel9y-1 (GH9 endoglucanase)—carboxymethylcellulose49.2120.852.36pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), carboxymethylcellulose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—cellohexaose41.75.1068.161pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), cellohexaose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—cellopentaoseTable 142.270.61568.73pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), cellopentaose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—cellopentaose200 mM D-galactose added; Table 235.570.48373.64pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), cellopentaose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—cellopentaose1000 mM D-galactose added; Table 247.810.253189.0pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), cellopentaose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—cellopentaose200 mM D-arabinose added; Table 256.911.12250.72pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), cellopentaose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—cellopentaose1000 mM D-arabinose added; Table 277.780.91784.82pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), cellopentaose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—cellopentaoseTable 230.460.205148.6pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), cellopentaose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—cellopentaoseTable 245.570.169269.6pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), cellopentaose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—cellopentaose200 mM D-Xylose added; Table 245.570.446102.2pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), cellopentaose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—cellopentaose1000 mM D-Xylose added; Table 270.710.109648.7pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), cellopentaose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—cellotetraose159.818.38.733pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), cellotetraose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—cellotriose897.41525.917pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), cellotriose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—hydroxyethyl cellulose185.728.266.569pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), hydroxyethyl cellulose
β-glucanase Umcel9y-1 (GH9 endoglucanase)—laminarin from Eisenia bicyclis127.547.32.696pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), laminarin from Eisenia bicyclis
β-glucanase Umcel9y-1 (GH9 endoglucanase)—p-nitrophenyl β-D-cellobioside4671.381337.9pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), p-nitrophenyl β-D-cellobioside
β-glucanase Umcel9y-1 (GH9 endoglucanase)—β-D-glucan from barleyTable 1327613.31246.1pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), β-D-glucan from barley
β-glucanase Umcel9y-1 (GH9 endoglucanase)—β-D-glucan from barleyno additive; Table 2—13.7239.2pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), β-D-glucan from barley
β-glucanase Umcel9y-1 (GH9 endoglucanase)—β-D-glucan from barleywith 100 mM D-galactose added; Table 2972.25.654171.9pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), β-D-glucan from barley
β-glucanase Umcel9y-1 (GH9 endoglucanase)—β-D-glucan from barleywith 100 mM D-arabinose added; Table 2972.24.404220.8pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), β-D-glucan from barley
β-glucanase Umcel9y-1 (GH9 endoglucanase)—β-D-glucan from barleyTable 2897.45.378166.9pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), β-D-glucan from barley
β-glucanase Umcel9y-1 (GH9 endoglucanase)—β-D-glucan from barleywith 100 mM D-Xylose added; Table 2972.23.706262.3pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), β-D-glucan from barley
β-glucanase Umcel9y-1 (GH9 endoglucanase)—β-D-glucan from barleywith 100 mM D-glucose added; Table 2145814.04103.9pH 7, 37 °CPMID 26877766Open record: β-glucanase Umcel9y-1 (GH9 endoglucanase), β-D-glucan from barley