Values CatLog read from this paper
Probing the beta-1,3:1,4 glucanase, CtLic26A, with a thio-oligosaccharide and enzyme variants
PMID 18292875doi:10.1039/b719288fOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (36 values) from this paper: CtLic26A (family GH26 β-1,3:1,4 glucanase) from Clostridium thermocellum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CtLic26A (family GH26 β-1,3:1,4 glucanase) | Clostridium thermocellum | Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | 0.38 | 0.11 | 3.42 | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase), Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | |
| CtLic26A (family GH26 β-1,3:1,4 glucanase) | Clostridium thermocellum | high viscosity β-glucan (Megazyme) | 1.23 × 10³ | — | — | pH 7, 60 °C | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase), high viscosity β-glucan (Megazyme) |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant E258A | Clostridium thermocellum | Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | 0.027 | 0.188 | 0.138 | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant E258A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant E258A | Clostridium thermocellum | high viscosity β-glucan (Megazyme) | 430 | — | — | pH 7, 60 °C | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant E258A, high viscosity β-glucan (Megazyme) |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant E70A | Clostridium thermocellum | Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | 8.3 × 10⁻⁵ | 0.155 | 0.0053 | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant E70A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant E70A | Clostridium thermocellum | high viscosity β-glucan (Megazyme) | 135 | — | — | pH 7, 60 °C | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant E70A, high viscosity β-glucan (Megazyme) |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant F41A | Clostridium thermocellum | Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | 1.7 × 10⁻⁵ | 0.139 | 1.7 × 10⁻⁵ | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant F41A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant F41A | Clostridium thermocellum | high viscosity β-glucan (Megazyme) | 27 | — | — | pH 7, 60 °C | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant F41A, high viscosity β-glucan (Megazyme) |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant K260A | Clostridium thermocellum | Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | 0.053 | 1.162 | 0.047 | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant K260A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant K260A | Clostridium thermocellum | high viscosity β-glucan (Megazyme) | 83 | — | — | pH 7, 60 °C | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant K260A, high viscosity β-glucan (Megazyme) |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant N157A | Clostridium thermocellum | Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | 0.0122 | 0.011 | 1.07 | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant N157A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant N157A | Clostridium thermocellum | high viscosity β-glucan (Megazyme) | 1.3 × 10³ | — | — | pH 7, 60 °C | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant N157A, high viscosity β-glucan (Megazyme) |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant Q18A | Clostridium thermocellum | Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | 0.035 | 1.763 | 0.02 | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant Q18A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant Q18A | Clostridium thermocellum | high viscosity β-glucan (Megazyme) | 380 | — | — | pH 7, 60 °C | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant Q18A, high viscosity β-glucan (Megazyme) |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant W114A | Clostridium thermocellum | Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | — | — | 0.0027 | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant W114A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant W114A | Clostridium thermocellum | high viscosity β-glucan (Megazyme) | 1.42 × 10³ | — | — | pH 7, 60 °C | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant W114A, high viscosity β-glucan (Megazyme) |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant W72A | Clostridium thermocellum | Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | — | — | 0.011 | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant W72A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant W72A | Clostridium thermocellum | high viscosity β-glucan (Megazyme) | 2.25 × 10³ | — | — | pH 7, 60 °C | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant W72A, high viscosity β-glucan (Megazyme) |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant Y115A | Clostridium thermocellum | Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | 8.3 × 10⁻⁴ | 0.26 | 0.0028 | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant Y115A, Glc-β1,4-Glc-β1,3-Glc-methylumbelliferyl | |
| CtLic26A (family GH26 β-1,3:1,4 glucanase)variant Y115A | Clostridium thermocellum | high viscosity β-glucan (Megazyme) | 250 | — | — | pH 7, 60 °C | Open record: CtLic26A (family GH26 β-1,3:1,4 glucanase) variant Y115A, high viscosity β-glucan (Megazyme) |