Values CatLog read from this paper
Site-directed mutagenesis of the putative active site of endoglucanase K from Bacillus sp. KSM-330
PMID 8075149doi:10.1016/0167-4838(94)00060-3Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (12 values) from this paper: endoglucanase K (EGK) from Bacillus sp. KSM-330.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| endoglucanase K (EGK) | Bacillus sp. KSM-330 | p-nitrophenyl-beta-D-cellotrioside | 0.022 | 1.5 | 0.014 calc. | pH 5.2, 40 °C | Open record: endoglucanase K (EGK), p-nitrophenyl-beta-D-cellotrioside |
| endoglucanase K (EGK)variant D154N | Bacillus sp. KSM-330 | p-nitrophenyl-beta-D-cellotrioside | 0.022 | 1.5 | 0.014 calc. | pH 5.2, 40 °C | Open record: endoglucanase K (EGK) variant D154N, p-nitrophenyl-beta-D-cellotrioside |
| endoglucanase K (EGK)variant D191N | Bacillus sp. KSM-330 | p-nitrophenyl-beta-D-cellotrioside | 1.7 × 10⁻⁵ | 2.1 | 7.9 × 10⁻⁶ calc. | pH 5.2, 40 °C | Open record: endoglucanase K (EGK) variant D191N, p-nitrophenyl-beta-D-cellotrioside |
| endoglucanase K (EGK)variant D193N | Bacillus sp. KSM-330 | p-nitrophenyl-beta-D-cellotrioside | 5 × 10⁻⁴ | 2.1 | 2.4 × 10⁻⁴ calc. | pH 5.2, 40 °C | Open record: endoglucanase K (EGK) variant D193N, p-nitrophenyl-beta-D-cellotrioside |
| endoglucanase K (EGK)variant D300N | Bacillus sp. KSM-330 | p-nitrophenyl-beta-D-cellotrioside | 0.0012 | 2 | 5.8 × 10⁻⁴ calc. | pH 5.2, 40 °C | Open record: endoglucanase K (EGK) variant D300N, p-nitrophenyl-beta-D-cellotrioside |
| endoglucanase K (EGK)variant E130Q | Bacillus sp. KSM-330 | p-nitrophenyl-beta-D-cellotrioside | 3.3 × 10⁻⁵ | 1.7 | 2.0 × 10⁻⁵ calc. | pH 5.2, 40 °C | Open record: endoglucanase K (EGK) variant E130Q, p-nitrophenyl-beta-D-cellotrioside |