Values CatLog read from this paper
Divergent evolution of function in the ROK sugar kinase superfamily: role of enzyme loops in substrate specificity
PMID 17979299doi:10.1021/bi700924dOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 35 records (75 values) from this paper: allokinase (AlsK), N-acetyl-D-mannosamine kinase (NanK) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| allokinase (AlsK) | Escherichia coli | 2-deoxy-D-glucose | 1.5 | 380 | 0.0041 | pH 7.6, 25 °C | Open record: allokinase (AlsK), 2-deoxy-D-glucose |
| allokinase (AlsK) | Escherichia coli | ATP5 mM phosphoenolpyruvate; 5 mM KCl; 6-26 mM MgCl2 | — | 0.27 | — | pH 7.6, 25 °C | Open record: allokinase (AlsK), ATP |
| allokinase (AlsK) | Escherichia coli | ATP8.5 mM MgCl2; cosubstrate D-glucose | — | 5.4 | — | pH 7.6, 25 °C | Open record: allokinase (AlsK), ATP |
| allokinase (AlsK) | Escherichia coli | D-allose | 47 | 0.19 | 250 | pH 7.6, 25 °C | Open record: allokinase (AlsK), D-allose |
| allokinase (AlsK) | Escherichia coli | D-altrose | 34 | 210 | 0.16 | pH 7.6, 25 °C | Open record: allokinase (AlsK), D-altrose |
| allokinase (AlsK) | Escherichia coli | D-glucose | 10 | 29 | 0.34 | pH 7.6, 25 °C | Open record: allokinase (AlsK), D-glucose |
| allokinase (AlsK) | Escherichia coli | D-mannose | 0.5 | 390 | 0.0012 | pH 7.6, 25 °C | Open record: allokinase (AlsK), D-mannose |
| allokinase (AlsK)variant A73G | Escherichia coli | 2-deoxy-D-glucose | 19 | 51 | 0.37 | pH 7.6, 25 °C | Open record: allokinase (AlsK) variant A73G, 2-deoxy-D-glucose |
| allokinase (AlsK)variant A73G | Escherichia coli | ATP5 mM phosphoenolpyruvate; 5 mM KCl; 6-26 mM MgCl2 | — | 0.06 | — | pH 7.6, 25 °C | Open record: allokinase (AlsK) variant A73G, ATP |
| allokinase (AlsK)variant A73G | Escherichia coli | ATP8.5 mM MgCl2; cosubstrate D-glucose | — | 0.45 | — | pH 7.6, 25 °C | Open record: allokinase (AlsK) variant A73G, ATP |
| allokinase (AlsK)variant A73G | Escherichia coli | D-allose | 40 | 0.2 | 200 | pH 7.6, 25 °C | Open record: allokinase (AlsK) variant A73G, D-allose |
| allokinase (AlsK)variant A73G | Escherichia coli | D-altrose | 94 | 48 | 2 | pH 7.6, 25 °C | Open record: allokinase (AlsK) variant A73G, D-altrose |
| allokinase (AlsK)variant A73G | Escherichia coli | D-glucose | 13 | 0.65 | 21 | pH 7.6, 25 °C | Open record: allokinase (AlsK) variant A73G, D-glucose |
| allokinase (AlsK)variant A73G | Escherichia coli | D-mannose | 9.5 | 210 | 0.045 | pH 7.6, 25 °C | Open record: allokinase (AlsK) variant A73G, D-mannose |
| allokinase (AlsK)variant F145L | Escherichia coli | ATP5 mM phosphoenolpyruvate; 5 mM KCl; 6-26 mM MgCl2 | — | 0.14 | — | pH 7.6, 25 °C | Open record: allokinase (AlsK) variant F145L, ATP |
| allokinase (AlsK)variant F145L | Escherichia coli | ATP8.5 mM MgCl2; cosubstrate D-glucose | — | 0.96 | — | pH 7.6, 25 °C | Open record: allokinase (AlsK) variant F145L, ATP |
| allokinase (AlsK)variant F145L | Escherichia coli | D-allose | 49 | 0.15 | 320 | pH 7.6, 25 °C | Open record: allokinase (AlsK) variant F145L, D-allose |
| allokinase (AlsK)variant F145L | Escherichia coli | D-glucose | 28 | 7.2 | 3.9 | pH 7.6, 25 °C | Open record: allokinase (AlsK) variant F145L, D-glucose |
| N-acetyl-D-mannosamine kinase (NanK) | Escherichia coli | 1,5-anhydroglucitol | — | — | 0.0017 | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK), 1,5-anhydroglucitol |
| N-acetyl-D-mannosamine kinase (NanK) | Escherichia coli | ATP5 mM phosphoenolpyruvate; 5 mM KCl; 6-26 mM MgCl2 | — | 0.26 | — | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK), ATP |
| N-acetyl-D-mannosamine kinase (NanK) | Escherichia coli | ATP8.5 mM MgCl2; cosubstrate D-glucose | — | 1.2 | — | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK), ATP |
| N-acetyl-D-mannosamine kinase (NanK) | Escherichia coli | D-glucose | 22 | 20 | 1.1 | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK), D-glucose |
| N-acetyl-D-mannosamine kinase (NanK) | Escherichia coli | D-mannose | 25 | 84 | 0.3 | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK), D-mannose |
| N-acetyl-D-mannosamine kinase (NanK) | Escherichia coli | N-acetyl-D-mannosamine | 56 | 0.36 | 150 | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK), N-acetyl-D-mannosamine |
| N-acetyl-D-mannosamine kinase (NanK)variant L84P | Escherichia coli | 1,5-anhydroglucitol | — | — | 0.01 | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK) variant L84P, 1,5-anhydroglucitol |
| N-acetyl-D-mannosamine kinase (NanK)variant L84P | Escherichia coli | ATP5 mM phosphoenolpyruvate; 5 mM KCl; 6-26 mM MgCl2 | — | 0.084 | — | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK) variant L84P, ATP |
| N-acetyl-D-mannosamine kinase (NanK)variant L84P | Escherichia coli | ATP8.5 mM MgCl2; cosubstrate D-glucose | — | 0.92 | — | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK) variant L84P, ATP |
| N-acetyl-D-mannosamine kinase (NanK)variant L84P | Escherichia coli | D-glucose | 84 | 6.4 | 13 | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK) variant L84P, D-glucose |
| N-acetyl-D-mannosamine kinase (NanK)variant L84P | Escherichia coli | D-mannose | 62 | 23 | 2.8 | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK) variant L84P, D-mannose |
| N-acetyl-D-mannosamine kinase (NanK)variant L84P | Escherichia coli | N-acetyl-D-mannosamine | 18 | 0.23 | 78 | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK) variant L84P, N-acetyl-D-mannosamine |
| N-acetyl-D-mannosamine kinase (NanK)variant L84P/V138M | Escherichia coli | D-glucose | — | — | 5.3 | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK) variant L84P/V138M, D-glucose |
| N-acetyl-D-mannosamine kinase (NanK)variant V138M | Escherichia coli | ATP5 mM phosphoenolpyruvate; 5 mM KCl; 6-26 mM MgCl2 | — | 0.11 | — | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK) variant V138M, ATP |
| N-acetyl-D-mannosamine kinase (NanK)variant V138M | Escherichia coli | ATP8.5 mM MgCl2; cosubstrate D-glucose | — | 0.63 | — | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK) variant V138M, ATP |
| N-acetyl-D-mannosamine kinase (NanK)variant V138M | Escherichia coli | D-glucose | 60 | 8.6 | 7 | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK) variant V138M, D-glucose |
| N-acetyl-D-mannosamine kinase (NanK)variant V138M | Escherichia coli | N-acetyl-D-mannosamine | 20 | 0.16 | 120 | pH 7.6, 25 °C | Open record: N-acetyl-D-mannosamine kinase (NanK) variant V138M, N-acetyl-D-mannosamine |