Values CatLog read from this paper
Characterization of the 2',3' cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage lambda phosphatase
PMID 17986465doi:10.1093/nar/gkm868Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 21 records (40 values) from this paper: CthPnkp (polynucleotide kinase-phosphatase), λ phosphatase (λ-Pase) from Clostridium thermocellum, bacteriophage lambda.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 19 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CthPnkp (polynucleotide kinase-phosphatase) | Clostridium thermocellum | 4-nitrophenyl phenylphosphonate | 27.8 | 67 | 0.42 calc. | pH 7 | Open record: CthPnkp (polynucleotide kinase-phosphatase), 4-nitrophenyl phenylphosphonate |
| CthPnkp (polynucleotide kinase-phosphatase) | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 8.93 | 18 | 0.50 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase), adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant D233E | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 3.25 | 62 | 0.052 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant D233E, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant D236A | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 4.53 | 7.8 | 0.58 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant D236A, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant D236E | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 6.03 | 18 | 0.34 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant D236E, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant D236N | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 5.77 | 6.6 | 0.87 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant D236N, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant D392A | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 1.77 | 100 | 0.018 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant D392A, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant D392E | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 15.97 | 18 | 0.89 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant D392E, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant D392N | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 18.7 | 31 | 0.60 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant D392N, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant H189A | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 1.45 | 29 | 0.050 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant H189A, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant H189D | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 34.3 | 20 | 1.7 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant H189D, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant H189E | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 17.17 | 28 | 0.61 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant H189E, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant H323A | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 9.1 | 32 | 0.28 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant H323A, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant H323Q | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 6.5 | 24 | 0.27 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant H323Q, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant H376D | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 3.2 | 3.9 | 0.82 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant H376D, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant H376N | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 5.13 | 4.7 | 1.1 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant H376N, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant R237A | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 8.55 | 77 | 0.11 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant R237A, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant R237K | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 26.17 | 100 | 0.26 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant R237K, adenosine 2',3'-cyclic monophosphate |
| CthPnkp (polynucleotide kinase-phosphatase)variant R237Q | Clostridium thermocellum | adenosine 2',3'-cyclic monophosphate | 20.2 | 60 | 0.34 calc. | pH 7.5, 45 °C | Open record: CthPnkp (polynucleotide kinase-phosphatase) variant R237Q, adenosine 2',3'-cyclic monophosphate |
| λ phosphatase (λ-Pase) | bacteriophage lambda | 4-nitrophenyl phosphate | — | 5 | — | Open record: λ phosphatase (λ-Pase), 4-nitrophenyl phosphate | |
| λ phosphatase (λ-Pase) | bacteriophage lambda | bis(4-nitrophenyl) phosphate | — | 10 | — | Open record: λ phosphatase (λ-Pase), bis(4-nitrophenyl) phosphate |