Values CatLog read from this paper
Substrate recognition and catalysis by the cofactor-independent dioxygenase DpgC
PMID 18004875doi:10.1021/bi701148bOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (16 values) from this paper: DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase) from Streptomyces toyocaensis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase) | Streptomyces toyocaensis | (3,5-dihydroxyphenylacetyl)coenzyme A | 0.172 | 0.0039 | 44 calc. | pH 7.5, 24 °C | Open record: DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase), (3,5-dihydroxyphenylacetyl)coenzyme A |
| DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase) | Streptomyces toyocaensis | (3-hydroxyphenylacetyl)coenzyme A | 0.1253 | 0.0044 | 28 calc. | pH 7.5, 24 °C | Open record: DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase), (3-hydroxyphenylacetyl)coenzyme A |
| DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase) | Streptomyces toyocaensis | (4-hydroxyphenylacetyl)coenzyme A | 0.222 | 0.851 | 0.261 calc. | pH 7.5, 24 °C | Open record: DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase), (4-hydroxyphenylacetyl)coenzyme A |
| DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase) | Streptomyces toyocaensis | phenylacetyl-coenzyme A | 0.064 | 0.102 | 0.63 calc. | pH 7.5, 24 °C | Open record: DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase), phenylacetyl-coenzyme A |
| DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase)variant E189Q | Streptomyces toyocaensis | (3,5-dihydroxyphenylacetyl)coenzyme A | 0.0853 | 0.064 | 1.3 calc. | pH 7.5, 24 °C | Open record: DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase) variant E189Q, (3,5-dihydroxyphenylacetyl)coenzyme A |
| DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase)variant E255Q | Streptomyces toyocaensis | (3,5-dihydroxyphenylacetyl)coenzyme A | 0.153 | 0.0036 | 42 calc. | pH 7.5, 24 °C | Open record: DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase) variant E255Q, (3,5-dihydroxyphenylacetyl)coenzyme A |
| DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase)variant Q299N | Streptomyces toyocaensis | (3,5-dihydroxyphenylacetyl)coenzyme A | 0.069 | 0.0025 | 28 calc. | pH 7.5, 24 °C | Open record: DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase) variant Q299N, (3,5-dihydroxyphenylacetyl)coenzyme A |
| DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase)variant R254K | Streptomyces toyocaensis | (3,5-dihydroxyphenylacetyl)coenzyme A | 0.0862 | 0.217 | 0.397 calc. | pH 7.5, 24 °C | Open record: DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase) variant R254K, (3,5-dihydroxyphenylacetyl)coenzyme A |