Values CatLog read from this paper
Structural basis for substrate specificity in group I nucleoside hydrolases
PMID 18361502doi:10.1021/bi702448sOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 21 records (62 values) from this paper: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB), URH1p (uridine nucleoside hydrolase) from Escherichia coli, Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| URH1p (uridine nucleoside hydrolase) | Saccharomyces cerevisiae | cytidine | 12 | 1.02 | 11.8 | pH 7.3, 37 °C | Open record: URH1p (uridine nucleoside hydrolase), cytidine |
| URH1p (uridine nucleoside hydrolase) | Saccharomyces cerevisiae | uridine | 117 | 0.63 | 186 | pH 7.3, 37 °C | Open record: URH1p (uridine nucleoside hydrolase), uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) | Escherichia coli | inosine | 0.086 | 2.34 | 0.037 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB), inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) | Escherichia coli | uridine | 5.4 | 0.12 | 43 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB), uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant Q227A | Escherichia coli | inosine | 0.182 | 4.31 | 0.042 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant Q227A, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant Q227A | Escherichia coli | uridine | 46.9 | 0.77 | 60 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant Q227A, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant Q227F | Escherichia coli | inosine | 0.125 | 1.77 | 0.071 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant Q227F, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant Q227F | Escherichia coli | uridine | 52.5 | 1.19 | 44 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant Q227F, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant Q227Y | Escherichia coli | inosine | 0.593 | 2.14 | 0.278 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant Q227Y, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant Q227Y | Escherichia coli | uridine | 15.1 | 0.57 | 26 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant Q227Y, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223A | Escherichia coli | inosine | 0.18 | 3.29 | 0.055 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223A, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223A | Escherichia coli | uridine | 39.8 | 0.33 | 120 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223A, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223F | Escherichia coli | inosine | 0.109 | 5.3 | 0.021 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223F, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223F | Escherichia coli | uridine | 18.8 | 0.31 | 60 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223F, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223F/Q227Y | Escherichia coli | inosine | 0.382 | 4.42 | 0.0864 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223F/Q227Y, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223F/Q227Y | Escherichia coli | uridine | 59.1 | 1.09 | 54 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223F/Q227Y, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223Y | Escherichia coli | inosine | 0.035 | 1.16 | 0.03 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223Y, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223Y | Escherichia coli | uridine | 44.3 | 1.13 | 39 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223Y, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223Y/Q227Y | Escherichia coli | adenosine | 0.41 | 3.75 | 0.11 calc. | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223Y/Q227Y, adenosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223Y/Q227Y | Escherichia coli | inosine | 3.62 | 1.93 | 1.88 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223Y/Q227Y, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223Y/Q227Y | Escherichia coli | uridine | 72.9 | 1.06 | 68 | pH 7.3, 37 °C | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223Y/Q227Y, uridine |