EC 3.2.2.8: ribosylpyrimidine nucleosidase
HydrolasesOpen in Browse
CatLog read 50 records for EC 3.2.2.8 from 4 papers, for 4 organisms. Names in these papers: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB), nucleoside hydrolase (CeNH), ribonucleoside hydrolase C (LreRihC), inosine-uridine nucleoside hydrolase (LbIU-NH).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 25 records for EC 3.2.2.8 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.2.8 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Structural basis for substrate specificity in group I nucleoside hydrolases | Escherichia coli | 19 |
| Structural and biochemical characterization of the nucleoside hydrolase from C. elegans reveals the role of two active site cysteine residues in catalysis | Caenorhabditis elegans | 15 |
| Structure-Functional Examination of Novel Ribonucleoside Hydrolase C (RihC) from Limosilactobacillus reuteri LR1 | Limosilactobacillus reuteri | 9 |
| doi:10.1039/c7ra07268f | Leishmania braziliensis | 7 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| inosine-uridine nucleoside hydrolase (LbIU-NH) | Leishmania braziliensis | 4-nitrophenyl beta-D-ribofuranoside | 344 | 0.25 | 1.3 × 10³ | pH 7.5, 25 °C | doi:10.1039/c7ra07268f | Open record: inosine-uridine nucleoside hydrolase (LbIU-NH), 4-nitrophenyl beta-D-ribofuranoside |
| inosine-uridine nucleoside hydrolase (LbIU-NH) | Leishmania braziliensis | adenosine | 0.039 | 0.6 | 0.065 | pH 7.5, 25 °C | doi:10.1039/c7ra07268f | Open record: inosine-uridine nucleoside hydrolase (LbIU-NH), adenosine |
| inosine-uridine nucleoside hydrolase (LbIU-NH) | Leishmania braziliensis | cytidine | 0.066 | 0.7 | 0.094 | pH 7.5, 25 °C | doi:10.1039/c7ra07268f | Open record: inosine-uridine nucleoside hydrolase (LbIU-NH), cytidine |
| inosine-uridine nucleoside hydrolase (LbIU-NH) | Leishmania braziliensis | guanosine | — | — | 0.047 | pH 7.5, 25 °C | doi:10.1039/c7ra07268f | Open record: inosine-uridine nucleoside hydrolase (LbIU-NH), guanosine |
| inosine-uridine nucleoside hydrolase (LbIU-NH) | Leishmania braziliensis | inosine | 11.2 | 0.5 | 22 | pH 7.5, 25 °C | doi:10.1039/c7ra07268f | Open record: inosine-uridine nucleoside hydrolase (LbIU-NH), inosine |
| inosine-uridine nucleoside hydrolase (LbIU-NH) | Leishmania braziliensis | inosine | 11.3 | — | — | pH 7.5 | doi:10.1039/c7ra07268f | Open record: inosine-uridine nucleoside hydrolase (LbIU-NH), inosine |
| inosine-uridine nucleoside hydrolase (LbIU-NH) | Leishmania braziliensis | uridine | 5.1 | — | — | pH 7.5, 25 °C | doi:10.1039/c7ra07268f | Open record: inosine-uridine nucleoside hydrolase (LbIU-NH), uridine |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | 4-nitrophenyl beta-D-ribofuranoside | 0.039 | 3.2 × 10⁻⁴ | 121.9 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH), 4-nitrophenyl beta-D-ribofuranoside |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | 7-Methylguanosine | 1.09 | 0.037 | 29.46 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH), 7-Methylguanosine |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | Adenosine | 0.418 | 0.077 | 5.429 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH), Adenosine |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | Guanosine | 0.143 | 0.068 | 2.103 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH), Guanosine |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | Inosine | 0.203 | 0.129 | 1.574 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH), Inosine |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | 4-nitrophenyl beta-D-ribofuranoside | 0.017 | 0.009 | 1.889 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C253A, 4-nitrophenyl beta-D-ribofuranoside |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | 7-Methylguanosine | 1.98 | 0.051 | 38.82 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C253A, 7-Methylguanosine |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | Adenosine | 0.0042 | 0.096 | 0.044 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C253A, Adenosine |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | Guanosine | 0.0051 | 0.3 | 0.017 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C253A, Guanosine |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | Inosine | 0.0043 | 0.343 | 0.013 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C253A, Inosine |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | 4-nitrophenyl beta-D-ribofuranoside | 0.026 | 6.4 × 10⁻⁴ | 40.63 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C42A, 4-nitrophenyl beta-D-ribofuranoside |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | 7-Methylguanosine | 2.4 | 0.053 | 45.28 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C42A, 7-Methylguanosine |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | Adenosine | 0.023 | 0.094 | 0.245 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C42A, Adenosine |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | Guanosine | 0.011 | 0.054 | 0.202 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C42A, Guanosine |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | Inosine | 0.024 | 0.215 | 0.112 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C42A, Inosine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | adenosineN-terminal His6-tag (LreRihC_HisN) | 118 | 0.42 | 280 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), adenosine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | adenosineC-terminal His6-tag (LreRihC_HisC) | 65 | 0.48 | 140 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), adenosine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | cytidineN-terminal His6-tag (LreRihC_HisN) | 112 | 0.68 | 160 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), cytidine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | cytidineC-terminal His6-tag (LreRihC_HisC) | 58 | 0.62 | 94 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), cytidine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | inosineN-terminal His6-tag (LreRihC_HisN) | 30 | 2.5 | 12 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), inosine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | inosineC-terminal His6-tag (LreRihC_HisC) | 18 | 2.6 | 6.9 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), inosine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | uridine | 167 | 0.32 | 520 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), uridine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | xanthosineN-terminal His6-tag (LreRihC_HisN) | 57 | 1.2 | 48 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), xanthosine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | xanthosineC-terminal His6-tag (LreRihC_HisC) | 40 | 1.3 | 31 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), xanthosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) | Escherichia coli | inosine | 0.086 | 2.34 | 0.037 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB), inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) | Escherichia coli | uridine | 5.4 | 0.12 | 43 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB), uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant Q227A | Escherichia coli | inosine | 0.182 | 4.31 | 0.042 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant Q227A, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant Q227A | Escherichia coli | uridine | 46.9 | 0.77 | 60 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant Q227A, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant Q227F | Escherichia coli | inosine | 0.125 | 1.77 | 0.071 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant Q227F, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant Q227F | Escherichia coli | uridine | 52.5 | 1.19 | 44 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant Q227F, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant Q227Y | Escherichia coli | inosine | 0.593 | 2.14 | 0.278 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant Q227Y, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant Q227Y | Escherichia coli | uridine | 15.1 | 0.57 | 26 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant Q227Y, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223A | Escherichia coli | inosine | 0.18 | 3.29 | 0.055 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223A, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223A | Escherichia coli | uridine | 39.8 | 0.33 | 120 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223A, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223F | Escherichia coli | inosine | 0.109 | 5.3 | 0.021 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223F, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223F | Escherichia coli | uridine | 18.8 | 0.31 | 60 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223F, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223F/Q227Y | Escherichia coli | inosine | 0.382 | 4.42 | 0.0864 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223F/Q227Y, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223F/Q227Y | Escherichia coli | uridine | 59.1 | 1.09 | 54 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223F/Q227Y, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223Y | Escherichia coli | inosine | 0.035 | 1.16 | 0.03 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223Y, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223Y | Escherichia coli | uridine | 44.3 | 1.13 | 39 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223Y, uridine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223Y/Q227Y | Escherichia coli | adenosine | 0.41 | 3.75 | 0.11 calc. | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223Y/Q227Y, adenosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223Y/Q227Y | Escherichia coli | inosine | 3.62 | 1.93 | 1.88 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223Y/Q227Y, inosine |
| YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB)variant T223Y/Q227Y | Escherichia coli | uridine | 72.9 | 1.06 | 68 | pH 7.3, 37 °C | PMID 18361502 | Open record: YeiK (pyrimidine-specific nucleoside hydrolase, CU-NH; RihB) variant T223Y/Q227Y, uridine |