Values CatLog read from this paper
Structural and biochemical characterization of the nucleoside hydrolase from C. elegans reveals the role of two active site cysteine residues in catalysis
PMID 28218438doi:10.1002/pro.3141Open in Browse
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CatLog read 15 records (45 values) from this paper: nucleoside hydrolase (CeNH) from Caenorhabditis elegans.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
EC 3.2.2.1, EC 3.2.2.2, EC 3.2.2.3, EC 3.2.2.7, EC 3.2.2.8
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | 4-nitrophenyl beta-D-ribofuranoside | 0.039 | 3.2 × 10⁻⁴ | 121.9 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH), 4-nitrophenyl beta-D-ribofuranoside |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | 7-Methylguanosine | 1.09 | 0.037 | 29.46 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH), 7-Methylguanosine |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | Adenosine | 0.418 | 0.077 | 5.429 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH), Adenosine |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | Guanosine | 0.143 | 0.068 | 2.103 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH), Guanosine |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | Inosine | 0.203 | 0.129 | 1.574 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH), Inosine |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | 4-nitrophenyl beta-D-ribofuranoside | 0.017 | 0.009 | 1.889 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH) variant C253A, 4-nitrophenyl beta-D-ribofuranoside |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | 7-Methylguanosine | 1.98 | 0.051 | 38.82 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH) variant C253A, 7-Methylguanosine |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | Adenosine | 0.0042 | 0.096 | 0.044 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH) variant C253A, Adenosine |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | Guanosine | 0.0051 | 0.3 | 0.017 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH) variant C253A, Guanosine |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | Inosine | 0.0043 | 0.343 | 0.013 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH) variant C253A, Inosine |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | 4-nitrophenyl beta-D-ribofuranoside | 0.026 | 6.4 × 10⁻⁴ | 40.63 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH) variant C42A, 4-nitrophenyl beta-D-ribofuranoside |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | 7-Methylguanosine | 2.4 | 0.053 | 45.28 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH) variant C42A, 7-Methylguanosine |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | Adenosine | 0.023 | 0.094 | 0.245 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH) variant C42A, Adenosine |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | Guanosine | 0.011 | 0.054 | 0.202 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH) variant C42A, Guanosine |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | Inosine | 0.024 | 0.215 | 0.112 | pH 7.5, 25 °C | Open record: nucleoside hydrolase (CeNH) variant C42A, Inosine |