EC 3.2.2.1: purine nucleosidase
HydrolasesOpen in Browse
CatLog read 65 records for EC 3.2.2.1 from 6 papers, for 4 organisms. Names in these papers: nucleoside hydrolase (CeNH), inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), nucleoside hydrolase (TvNH, purine-specific), ribonucleoside hydrolase C (LreRihC) and 2 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 48 records for EC 3.2.2.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.2.1 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Structural and biochemical characterization of the nucleoside hydrolase from C. elegans reveals the role of two active site cysteine residues in catalysis | Caenorhabditis elegans | 15 |
| Structure and mechanism of the 6-oxopurine nucleosidase from Trypanosoma brucei brucei | Trypanosoma brucei brucei | 14 |
| A flexible loop as a functional element in the catalytic mechanism of nucleoside hydrolase from Trypanosoma vivax | Trypanosoma vivax | 13 |
| Structure-Functional Examination of Novel Ribonucleoside Hydrolase C (RihC) from Limosilactobacillus reuteri LR1 | Limosilactobacillus reuteri | 9 |
| Structure and function of a novel purine specific nucleoside hydrolase from Trypanosoma vivax | Trypanosoma vivax | 7 |
| Transition-state complex of the purine-specific nucleoside hydrolase of T. vivax: enzyme conformational changes and implications for catalysis | Trypanosoma vivax | 7 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH) | Trypanosoma vivax | 4-nitrophenyl beta-D-ribofuranoside | 0.206 | 0.257 | 0.802 | pH 7.5, 35 °C | PMID 11292348 | Open record: inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH), 4-nitrophenyl beta-D-ribofuranoside |
| inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH) | Trypanosoma vivax | adenosine | 1.46 | 0.00854 | 171 | pH 7.2, 35 °C | PMID 11292348 | Open record: inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH), adenosine |
| inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH) | Trypanosoma vivax | cytidine | 0.338 | 0.925 | 0.366 | pH 7.2, 35 °C | PMID 11292348 | Open record: inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH), cytidine |
| inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH) | Trypanosoma vivax | guanosine | 1.9 | 0.00382 | 497 | pH 7.2, 35 °C | PMID 11292348 | Open record: inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH), guanosine |
| inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH) | Trypanosoma vivax | inosine | 5.19 | 0.00537 | 970 | pH 7.2, 35 °C | PMID 11292348 | Open record: inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH), inosine |
| inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH) | Trypanosoma vivax | inosine | 5.49 | 0.0052 | 1.05 × 10³ | pH 7.2, 35 °C | PMID 11292348 | Open record: inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH), inosine |
| inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH) | Trypanosoma vivax | uridine | 0.022 | 0.586 | 0.0375 | pH 7.2, 35 °C | PMID 11292348 | Open record: inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH), uridine |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | 2-aminopurine riboside | 0.57 | 8 × 10⁻⁴ | 700 | pH 7, 35 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), 2-aminopurine riboside |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | 3-deazaadenosine | 0.009 | 0.0022 | 41 | pH 7, 35 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), 3-deazaadenosine |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | 5'-deoxyadenosine | 0.078 | 0.24 | 0.33 | pH 7, 35 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), 5'-deoxyadenosine |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | 7-methylguanosine | 5.2 | — | — | pH 7, 5 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), 7-methylguanosine |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | adenosine | 0.237 | — | — | pH 7, 35 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), adenosine |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | cytidine | 0.52 | 0.271 | 1.9 | pH 7, 35 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), cytidine |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | ethenoadenosine | 6.9 | 0.0036 | 1.9 × 10³ | pH 7, 35 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), ethenoadenosine |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | guanosine | 37.6 | 0.0045 | 8.3 × 10³ | pH 7, 35 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), guanosine |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | guanosine | 1.3 | — | — | pH 7, 5 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), guanosine |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | inosine | 28 | 0.0019 | 1.5 × 10⁴ | pH 7, 35 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), inosine |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | p-nitrophenyl beta-D-ribofuranoside | 0.03 | 0.0011 | 27 | pH 7, 35 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), p-nitrophenyl beta-D-ribofuranoside |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | purine riboside | 0.248 | 6 × 10⁻⁴ | 380 | pH 7, 35 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), purine riboside |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | uridine | 0.0038 | 1.451 | 0.0026 | pH 7, 35 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), uridine |
| inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH) | Trypanosoma brucei brucei | xanthosine | 6.2 | 0.28 | 22 | pH 7, 35 °C | PMID 20825170 | Open record: inosine-guanosine-preferring nucleoside hydrolase (Tbb IG-NH), xanthosine |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | 4-nitrophenyl beta-D-ribofuranoside | 0.039 | 3.2 × 10⁻⁴ | 121.9 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH), 4-nitrophenyl beta-D-ribofuranoside |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | 7-Methylguanosine | 1.09 | 0.037 | 29.46 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH), 7-Methylguanosine |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | Adenosine | 0.418 | 0.077 | 5.429 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH), Adenosine |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | Guanosine | 0.143 | 0.068 | 2.103 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH), Guanosine |
| nucleoside hydrolase (CeNH) | Caenorhabditis elegans | Inosine | 0.203 | 0.129 | 1.574 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH), Inosine |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | 4-nitrophenyl beta-D-ribofuranoside | 0.017 | 0.009 | 1.889 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C253A, 4-nitrophenyl beta-D-ribofuranoside |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | 7-Methylguanosine | 1.98 | 0.051 | 38.82 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C253A, 7-Methylguanosine |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | Adenosine | 0.0042 | 0.096 | 0.044 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C253A, Adenosine |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | Guanosine | 0.0051 | 0.3 | 0.017 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C253A, Guanosine |
| nucleoside hydrolase (CeNH)variant C253A | Caenorhabditis elegans | Inosine | 0.0043 | 0.343 | 0.013 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C253A, Inosine |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | 4-nitrophenyl beta-D-ribofuranoside | 0.026 | 6.4 × 10⁻⁴ | 40.63 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C42A, 4-nitrophenyl beta-D-ribofuranoside |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | 7-Methylguanosine | 2.4 | 0.053 | 45.28 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C42A, 7-Methylguanosine |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | Adenosine | 0.023 | 0.094 | 0.245 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C42A, Adenosine |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | Guanosine | 0.011 | 0.054 | 0.202 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C42A, Guanosine |
| nucleoside hydrolase (CeNH)variant C42A | Caenorhabditis elegans | Inosine | 0.024 | 0.215 | 0.112 | pH 7.5, 25 °C | PMID 28218438 | Open record: nucleoside hydrolase (CeNH) variant C42A, Inosine |
| nucleoside hydrolase (TvNH, purine-specific) | Trypanosoma vivax | 2-aminopurine riboside | 0.57 | 0.009 | 600 | pH 7, 25 °C | PMID 18519562 | Open record: nucleoside hydrolase (TvNH, purine-specific), 2-aminopurine riboside |
| nucleoside hydrolase (TvNH, purine-specific) | Trypanosoma vivax | 7-methylguanosine | 1.07 | 5 × 10⁻⁴ | 2.1 × 10⁴ | pH 7, 25 °C | PMID 18519562 | Open record: nucleoside hydrolase (TvNH, purine-specific), 7-methylguanosine |
| nucleoside hydrolase (TvNH, purine-specific) | Trypanosoma vivax | guanosine | 2.3 | 0.0023 | 1 × 10³ | pH 7, 25 °C | PMID 18519562 | Open record: nucleoside hydrolase (TvNH, purine-specific), guanosine |
| nucleoside hydrolase (TvNH, purine-specific)3G (loop 2 deletion: 13 residues between Met244 and Tyr258 replaced by three Gly) | Trypanosoma vivax | 2-aminopurine riboside | 0.22 | 0.03 | 70 | pH 7, 25 °C | PMID 18519562 | Open record: nucleoside hydrolase (TvNH, purine-specific) 3G (loop 2 deletion: 13 residues between Met244 and Tyr258 replaced by three Gly), 2-aminopurine riboside |
| nucleoside hydrolase (TvNH, purine-specific)3G (loop 2 deletion: 13 residues between Met244 and Tyr258 replaced by three Gly) | Trypanosoma vivax | 7-methylguanosine | 56 | 0.015 | 3.7 × 10⁴ | pH 7, 25 °C | PMID 18519562 | Open record: nucleoside hydrolase (TvNH, purine-specific) 3G (loop 2 deletion: 13 residues between Met244 and Tyr258 replaced by three Gly), 7-methylguanosine |
| nucleoside hydrolase (TvNH, purine-specific)3G (loop 2 deletion: 13 residues between Met244 and Tyr258 replaced by three Gly) | Trypanosoma vivax | guanosine | 1.52 | 0.266 | 6 | pH 7, 25 °C | PMID 18519562 | Open record: nucleoside hydrolase (TvNH, purine-specific) 3G (loop 2 deletion: 13 residues between Met244 and Tyr258 replaced by three Gly), guanosine |
| nucleoside hydrolase (TvNH, purine-specific)variant C248P | Trypanosoma vivax | guanosine | 1.09 | 0.004 | 270 | pH 7, 25 °C | PMID 18519562 | Open record: nucleoside hydrolase (TvNH, purine-specific) variant C248P, guanosine |
| nucleoside hydrolase (TvNH, purine-specific)variant D253P | Trypanosoma vivax | guanosine | 3.9 | 0.0018 | 2.2 × 10³ | pH 7, 25 °C | PMID 18519562 | Open record: nucleoside hydrolase (TvNH, purine-specific) variant D253P, guanosine |
| nucleoside hydrolase (TvNH, purine-specific)variant E249P | Trypanosoma vivax | guanosine | 1.7 | 0.003 | 400 | pH 7, 25 °C | PMID 18519562 | Open record: nucleoside hydrolase (TvNH, purine-specific) variant E249P, guanosine |
| nucleoside hydrolase (TvNH, purine-specific)variant H247P | Trypanosoma vivax | guanosine | 4.1 | 0.008 | 500 | pH 7, 25 °C | PMID 18519562 | Open record: nucleoside hydrolase (TvNH, purine-specific) variant H247P, guanosine |
| nucleoside hydrolase (TvNH, purine-specific)variant L250P | Trypanosoma vivax | guanosine | 1.4 | 0.016 | 80 | pH 7, 25 °C | PMID 18519562 | Open record: nucleoside hydrolase (TvNH, purine-specific) variant L250P, guanosine |
| nucleoside hydrolase (TvNH, purine-specific)variant L251P | Trypanosoma vivax | guanosine | 2.5 | 0.021 | 120 | pH 7, 25 °C | PMID 18519562 | Open record: nucleoside hydrolase (TvNH, purine-specific) variant L251P, guanosine |
| nucleoside hydrolase (TvNH, purine-specific)variant R252P | Trypanosoma vivax | guanosine | 13 | 0.024 | 500 | pH 7, 25 °C | PMID 18519562 | Open record: nucleoside hydrolase (TvNH, purine-specific) variant R252P, guanosine |
| purine-specific nucleoside hydrolase (IAG-NH, TvNH)variant D40A | Trypanosoma vivax | 7-methylguanosine | 13.6 | 0.008 | 1.7 × 10³ calc. | pH 7, 35 °C | PMID 16630632 | Open record: purine-specific nucleoside hydrolase (IAG-NH, TvNH) variant D40A, 7-methylguanosine |
| purine-specific nucleoside hydrolase (IAG-NH, TvNH)variant D40A | Trypanosoma vivax | guanosine | 2.7 | 0.066 | 41 calc. | pH 7, 35 °C | PMID 16630632 | Open record: purine-specific nucleoside hydrolase (IAG-NH, TvNH) variant D40A, guanosine |
| purine-specific nucleoside hydrolase (IAG-NH, TvNH)variant R252A | Trypanosoma vivax | 7-methylguanosine | 13.2 | 0.0024 | 5.5 × 10³ calc. | pH 7, 35 °C | PMID 16630632 | Open record: purine-specific nucleoside hydrolase (IAG-NH, TvNH) variant R252A, 7-methylguanosine |
| purine-specific nucleoside hydrolase (IAG-NH, TvNH)variant R252A | Trypanosoma vivax | adenosine | 5.7 | 0.0066 | 860 calc. | pH 7, 35 °C | PMID 16630632 | Open record: purine-specific nucleoside hydrolase (IAG-NH, TvNH) variant R252A, adenosine |
| purine-specific nucleoside hydrolase (IAG-NH, TvNH)variant R252A | Trypanosoma vivax | cytidine | 0.54 | 1.06 | 0.51 calc. | pH 7, 35 °C | PMID 16630632 | Open record: purine-specific nucleoside hydrolase (IAG-NH, TvNH) variant R252A, cytidine |
| purine-specific nucleoside hydrolase (IAG-NH, TvNH)variant R252A | Trypanosoma vivax | guanosine | 9.8 | 0.031 | 320 calc. | pH 7, 35 °C | PMID 16630632 | Open record: purine-specific nucleoside hydrolase (IAG-NH, TvNH) variant R252A, guanosine |
| purine-specific nucleoside hydrolase (IAG-NH, TvNH)variant R252A | Trypanosoma vivax | purine riboside | 2 | 0.011 | 180 calc. | pH 7, 35 °C | PMID 16630632 | Open record: purine-specific nucleoside hydrolase (IAG-NH, TvNH) variant R252A, purine riboside |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | adenosineN-terminal His6-tag (LreRihC_HisN) | 118 | 0.42 | 280 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), adenosine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | adenosineC-terminal His6-tag (LreRihC_HisC) | 65 | 0.48 | 140 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), adenosine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | cytidineN-terminal His6-tag (LreRihC_HisN) | 112 | 0.68 | 160 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), cytidine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | cytidineC-terminal His6-tag (LreRihC_HisC) | 58 | 0.62 | 94 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), cytidine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | inosineN-terminal His6-tag (LreRihC_HisN) | 30 | 2.5 | 12 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), inosine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | inosineC-terminal His6-tag (LreRihC_HisC) | 18 | 2.6 | 6.9 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), inosine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | uridine | 167 | 0.32 | 520 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), uridine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | xanthosineN-terminal His6-tag (LreRihC_HisN) | 57 | 1.2 | 48 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), xanthosine |
| ribonucleoside hydrolase C (LreRihC) | Limosilactobacillus reuteri | xanthosineC-terminal His6-tag (LreRihC_HisC) | 40 | 1.3 | 31 calc. | pH 7.5 | PMID 38203708 | Open record: ribonucleoside hydrolase C (LreRihC), xanthosine |