CatLogenzyme kinetics catalog

Values CatLog read from this paper

Regiospecific O-methylation of naphthoic acids catalyzed by NcsB1, an O-methyltransferase involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin

PMID 18387946doi:10.1074/jbc.m802206200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 5 records (10 values) from this paper: NcsB1 (AdoMet-dependent O-methyltransferase) from Streptomyces carzinostaticus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.1.1.303

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
NcsB1 (AdoMet-dependent O-methyltransferase)Streptomyces carzinostaticus1,4-dihydroxy-2-naphthoic acid1.33 × 10⁻⁴0.0240.0056 calc.pH 6, 25 °COpen
NcsB1 (AdoMet-dependent O-methyltransferase)Streptomyces carzinostaticus2,7-dihydroxy-5-methyl-1-naphthoic acid0.01150.2060.0560 calc.pH 6, 25 °COpen
NcsB1 (AdoMet-dependent O-methyltransferase)Streptomyces carzinostaticus3,5-dihydroxy-2-naphthoic acid0.00450.3520.013 calc.pH 6, 25 °COpen
NcsB1 (AdoMet-dependent O-methyltransferase)Streptomyces carzinostaticus3,7-dihydroxy-2-naphthoic acid5 × 10⁻⁴0.0660.0076 calc.pH 6, 25 °COpen
NcsB1 (AdoMet-dependent O-methyltransferase)Streptomyces carzinostaticusS-adenosyl-L-methionine0.01250.0620.20 calc.pH 6, 25 °COpen