CatLogenzyme kinetics catalog

EC 2.1.1.303: 2,7-dihydroxy-5-methyl-1-naphthoate 7-O-methyltransferase

TransferasesOpen in Browse

CatLog read 9 records for EC 2.1.1.303 from 2 papers, for 1 organism. Names in these papers: NcsB1 (AdoMet-dependent O-methyltransferase), NcsB1 (SAM-dependent O-methyltransferase).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

PaperOrganismsRecords
Regiospecific O-methylation of naphthoic acids catalyzed by NcsB1, an O-methyltransferase involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatinStreptomyces carzinostaticus5
Molecular basis of substrate promiscuity for the SAM-dependent O-methyltransferase NcsB1, involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatinStreptomyces carzinostaticus4

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
NcsB1 (AdoMet-dependent O-methyltransferase)Streptomyces carzinostaticus1,4-dihydroxy-2-naphthoic acid1.33 × 10⁻⁴0.0240.0056 calc.pH 6, 25 °CPMID 18387946Open
NcsB1 (AdoMet-dependent O-methyltransferase)Streptomyces carzinostaticus2,7-dihydroxy-5-methyl-1-naphthoic acid0.01150.2060.0560 calc.pH 6, 25 °CPMID 18387946Open
NcsB1 (AdoMet-dependent O-methyltransferase)Streptomyces carzinostaticus3,5-dihydroxy-2-naphthoic acid0.00450.3520.013 calc.pH 6, 25 °CPMID 18387946Open
NcsB1 (AdoMet-dependent O-methyltransferase)Streptomyces carzinostaticus3,7-dihydroxy-2-naphthoic acid5 × 10⁻⁴0.0660.0076 calc.pH 6, 25 °CPMID 18387946Open
NcsB1 (AdoMet-dependent O-methyltransferase)Streptomyces carzinostaticusS-adenosyl-L-methionine0.01250.0620.20 calc.pH 6, 25 °CPMID 18387946Open
NcsB1 (SAM-dependent O-methyltransferase)variant R11AStreptomyces carzinostaticus2,7-dihydroxy-5-methyl-1-naphthoic acid0.02070.4190.0493 calc.pH 6.5, 25 °CPMID 19702337Open
NcsB1 (SAM-dependent O-methyltransferase)variant R11KStreptomyces carzinostaticus2,7-dihydroxy-5-methyl-1-naphthoic acid0.01970.3190.0617 calc.pH 6.5, 25 °CPMID 19702337Open
NcsB1 (SAM-dependent O-methyltransferase)variant R11WStreptomyces carzinostaticus2,7-dihydroxy-5-methyl-1-naphthoic acid0.010.40.025 calc.pH 6.5, 25 °CPMID 19702337Open
NcsB1 (SAM-dependent O-methyltransferase)variant Y293IStreptomyces carzinostaticus2,7-dihydroxy-5-methyl-1-naphthoic acid0.01270.6490.0200 calc.pH 6.5, 25 °CPMID 19702337Open