CatLogenzyme kinetics catalog

Values CatLog read from this paper

Molecular basis of substrate promiscuity for the SAM-dependent O-methyltransferase NcsB1, involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin

PMID 19702337doi:10.1021/bi901257qOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 4 records (8 values) from this paper: NcsB1 (SAM-dependent O-methyltransferase) from Streptomyces carzinostaticus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.1.1.303

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
NcsB1 (SAM-dependent O-methyltransferase)variant R11AStreptomyces carzinostaticus2,7-dihydroxy-5-methyl-1-naphthoic acid0.02070.4190.0493 calc.pH 6.5, 25 °COpen
NcsB1 (SAM-dependent O-methyltransferase)variant R11KStreptomyces carzinostaticus2,7-dihydroxy-5-methyl-1-naphthoic acid0.01970.3190.0617 calc.pH 6.5, 25 °COpen
NcsB1 (SAM-dependent O-methyltransferase)variant R11WStreptomyces carzinostaticus2,7-dihydroxy-5-methyl-1-naphthoic acid0.010.40.025 calc.pH 6.5, 25 °COpen
NcsB1 (SAM-dependent O-methyltransferase)variant Y293IStreptomyces carzinostaticus2,7-dihydroxy-5-methyl-1-naphthoic acid0.01270.6490.0200 calc.pH 6.5, 25 °COpen