Values CatLog read from this paper
Investigating a catalytic mechanism of hyperthermophilic L-threonine dehydrogenase from Pyrococcus horikoshii
PMID 18390572doi:10.1093/jb/mvn041Open in Browse
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CatLog read 16 records (32 values) from this paper: L-threonine dehydrogenase (PhTDH) from Pyrococcus horikoshii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| L-threonine dehydrogenase (PhTDH) | Pyrococcus horikoshii | L-threonine | 1.108 | 0.0118 | 94 | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH), L-threonine |
| L-threonine dehydrogenase (PhTDH) | Pyrococcus horikoshii | NAD+ | — | 0.0099 | — | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH), NAD+ |
| L-threonine dehydrogenase (PhTDH)variant E152A | Pyrococcus horikoshii | L-threonine | 0.00912 | 17.5 | 5.22 × 10⁻⁴ | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant E152A, L-threonine |
| L-threonine dehydrogenase (PhTDH)variant E152A | Pyrococcus horikoshii | NAD+ | — | 0.0615 | — | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant E152A, NAD+ |
| L-threonine dehydrogenase (PhTDH)variant E152C | Pyrococcus horikoshii | L-threonine | 0.242 | 0.308 | 0.787 | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant E152C, L-threonine |
| L-threonine dehydrogenase (PhTDH)variant E152C | Pyrococcus horikoshii | NAD+ | — | 0.163 | — | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant E152C, NAD+ |
| L-threonine dehydrogenase (PhTDH)variant E152D | Pyrococcus horikoshii | L-threonine | 3.5 | 0.237 | 14.77 | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant E152D, L-threonine |
| L-threonine dehydrogenase (PhTDH)variant E152D | Pyrococcus horikoshii | NAD+ | — | 0.399 | — | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant E152D, NAD+ |
| L-threonine dehydrogenase (PhTDH)variant E152Q | Pyrococcus horikoshii | L-threonine | 0.01028 | 50.6 | 2.03 × 10⁻⁴ | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant E152Q, L-threonine |
| L-threonine dehydrogenase (PhTDH)variant E152Q | Pyrococcus horikoshii | NAD+ | — | 0.216 | — | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant E152Q, NAD+ |
| L-threonine dehydrogenase (PhTDH)variant E152S | Pyrococcus horikoshii | L-threonine | 0.085 | 21.2 | 0.00402 | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant E152S, L-threonine |
| L-threonine dehydrogenase (PhTDH)variant E152S | Pyrococcus horikoshii | NAD+ | — | 0.607 | — | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant E152S, NAD+ |
| L-threonine dehydrogenase (PhTDH)variant E152T | Pyrococcus horikoshii | L-threonine | 0.0802 | 23.6 | 0.0034 | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant E152T, L-threonine |
| L-threonine dehydrogenase (PhTDH)variant E152T | Pyrococcus horikoshii | NAD+ | — | 0.152 | — | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant E152T, NAD+ |
| L-threonine dehydrogenase (PhTDH)variant R294A | Pyrococcus horikoshii | L-threonine | 0.1293 | 5.32 | 0.0243 | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant R294A, L-threonine |
| L-threonine dehydrogenase (PhTDH)variant R294A | Pyrococcus horikoshii | NAD+ | — | 0.105 | — | pH 7.5, 65 °C | Open record: L-threonine dehydrogenase (PhTDH) variant R294A, NAD+ |