CatLogenzyme kinetics catalog

Values CatLog read from this paper

Steady-state kinetics and mechanism of LpxD, the N-acyltransferase of lipid A biosynthesis

PMID 18422345doi:10.1021/bi800240rOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 24 records (35 values) from this paper: LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 11 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.3.1.191

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)Escherichia coliR-3-hydroxymyristoyl-acyl carrier proteineq. 8 fit230.00327.2 × 10³ calc.pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)Escherichia coliR-3-hydroxymyristoyl-acyl carrier proteinradiolabel TLC assay—0.0032—pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)Escherichia coliUDP-3-O-(R-3-hydroxymyristoyl)-alpha-D-glucosamineeq. 8 fit—0.0025—pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)Escherichia coliUDP-3-O-(R-3-hydroxymyristoyl)-alpha-D-glucosamineradiolabel TLC assay—0.0013—pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant D232AEscherichia coliR-3-hydroxymyristoyl-acyl carrier protein1.90.04740 calc.pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant D232AEscherichia coliUDP-3-O-(R-3-hydroxymyristoyl)-alpha-D-glucosamine—0.067—pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant F41AEscherichia coliR-3-hydroxymyristoyl-acyl carrier protein4.10.00351.2 × 10³ calc.pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant F41AEscherichia coliUDP-3-O-(R-3-hydroxymyristoyl)-alpha-D-glucosamine—0.073—pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant H239AEscherichia coliR-3-hydroxymyristoyl-acyl carrier protein0.0320.00349.4 calc.pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant H239AEscherichia coliUDP-3-O-(R-3-hydroxymyristoyl)-alpha-D-glucosamine—8.4 × 10⁻⁴—pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant H276AEscherichia coliR-3-hydroxymyristoyl-acyl carrier protein0.730.0017430 calc.pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant H276AEscherichia coliUDP-3-O-(R-3-hydroxymyristoyl)-alpha-D-glucosamine—0.0063—pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant K194AEscherichia coliR-3-hydroxymyristoyl-acyl carrier protein5.70.00381.5 × 10³ calc.pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant K194AEscherichia coliUDP-3-O-(R-3-hydroxymyristoyl)-alpha-D-glucosamine—0.0056—pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant K46AEscherichia coliR-3-hydroxymyristoyl-acyl carrier protein170.0121.4 × 10³ calc.pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant K46AEscherichia coliUDP-3-O-(R-3-hydroxymyristoyl)-alpha-D-glucosamine—0.0071—pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant N233AEscherichia coliR-3-hydroxymyristoyl-acyl carrier protein1.40.013110 calc.pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant N233AEscherichia coliUDP-3-O-(R-3-hydroxymyristoyl)-alpha-D-glucosamine—0.028—pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant Q236AEscherichia coliR-3-hydroxymyristoyl-acyl carrier protein120.013920 calc.pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant Q236AEscherichia coliUDP-3-O-(R-3-hydroxymyristoyl)-alpha-D-glucosamine—0.0042—pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant R293AEscherichia coliR-3-hydroxymyristoyl-acyl carrier protein8.90.074120 calc.pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant R293AEscherichia coliUDP-3-O-(R-3-hydroxymyristoyl)-alpha-D-glucosamine—0.0036—pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant Y47AEscherichia coliR-3-hydroxymyristoyl-acyl carrier protein180.0053.6 × 10³ calc.pH 7.5, 30 °COpen
LpxD (UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase)variant Y47AEscherichia coliUDP-3-O-(R-3-hydroxymyristoyl)-alpha-D-glucosamine—0.0078—pH 7.5, 30 °COpen