Values CatLog read from this paper
Residues Asp164 and Glu165 at the substrate entryway function potently in substrate orientation of alanine racemase from E. coli: Enzymatic characterization with crystal structure analysis
PMID 18434499doi:10.1110/ps.083495908Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (36 values) from this paper: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 18 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) | Escherichia coli | D-alanine | 5.78 | 0.311 | 18.6 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1), D-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) | Escherichia coli | L-alanine | 53.98 | 1.008 | 53.55 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1), L-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant D164A | Escherichia coli | D-alanine | 4.47 | 0.615 | 7.26 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant D164A, D-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant D164A | Escherichia coli | L-alanine | 41.82 | 3.03 | 13.8 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant D164A, L-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant D164K | Escherichia coli | D-alanine | 0.33 | 0.604 | 0.55 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant D164K, D-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant D164K | Escherichia coli | L-alanine | 2.8 | 3.603 | 0.78 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant D164K, L-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant E165A | Escherichia coli | D-alanine | 4 | 0.592 | 6.8 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant E165A, D-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant E165A | Escherichia coli | L-alanine | 22.47 | 1.562 | 14.38 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant E165A, L-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant E165K | Escherichia coli | D-alanine | 1.32 | 0.528 | 2.50 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant E165K, D-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant E165K | Escherichia coli | L-alanine | 16.72 | 2.057 | 8.127 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant E165K, L-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant E221A | Escherichia coli | D-alanine | 6.82 | 0.439 | 15.5 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant E221A, D-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant E221A | Escherichia coli | L-alanine | 70.92 | 1.516 | 46.78 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant E221A, L-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant E221K | Escherichia coli | D-alanine | 6.35 | 0.402 | 15.8 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant E221K, D-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant E221K | Escherichia coli | L-alanine | 68.23 | 1.993 | 34.24 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant E221K, L-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant E221P | Escherichia coli | D-alanine | 7.62 | 0.513 | 14.8 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant E221P, D-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant E221P | Escherichia coli | L-alanine | 67.07 | 1.401 | 47.87 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant E221P, L-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant P219A | Escherichia coli | D-alanine | 5.27 | 0.304 | 17.3 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant P219A, D-alanine |
| alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1)variant P219A | Escherichia coli | L-alanine | 51.77 | 1.049 | 49.35 calc. | pH 8, 30 °C | Open record: alanine racemase (EcAlr, biosynthetic alr-encoded alanine racemase 1) variant P219A, L-alanine |