Values CatLog read from this paper
The conformational switch from the factor X zymogen to protease state mediates exosite expression and prothrombinase assembly
PMID 18460471doi:10.1074/jbc.m802205200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (44 values) from this paper: factor Xa from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| factor Xa | Homo sapiens | methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilidefree FXa; 2.0 nM FXa with SpecXa 10-500 μM | 140 | 0.072 | 1.9 × 10³ calc. | pH 7.5 | Open record: factor Xa, methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide |
| factor Xa | Homo sapiens | methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide5.0 nM FXa with SpecXa 10-500 μM | 72 | 0.17 | 420 calc. | pH 7.5 | Open record: factor Xa, methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide |
| factor Xa | Homo sapiens | methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilidefree FXa; 2.0 nM FXa with SpecXa 10-500 μM | 210 | 0.089 | 2.4 × 10³ calc. | pH 7.5 | Open record: factor Xa, methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide |
| factor Xa | Homo sapiens | methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide5.0 nM FXa with SpecXa 10-500 μM | 140 | 0.13 | 1.1 × 10³ calc. | pH 7.5 | Open record: factor Xa, methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide |
| factor Xa | Homo sapiens | prethrombin-1plasma-derived factor X purified from human plasma | 18.13 | 0.0054 | 3.3 × 10³ | pH 7.5 | Open record: factor Xa, prethrombin-1 |
| factor Xa | Homo sapiens | prethrombin-1recombinant factor X expressed in HEK293 cells | 17.5 | 0.0052 | 3.4 × 10³ | pH 7.5 | Open record: factor Xa, prethrombin-1 |
| factor Xa | Homo sapiens | prothrombinplasma-derived factor X purified from human plasma | 40.4 | 4.2 × 10⁻⁴ | 9.6 × 10⁴ | pH 7.5 | Open record: factor Xa, prothrombin |
| factor Xa | Homo sapiens | prothrombinrecombinant factor X expressed in HEK293 cells | 32.28 | 3.5 × 10⁻⁴ | 9.2 × 10⁴ | pH 7.5 | Open record: factor Xa, prothrombin |
| factor Xavariant I16L | Homo sapiens | methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilidefree FXa; 20 mM Hepes; 0.15 M NaCl; 2 mM CaCl2; Table 1 | 58 | 1.15 | 50 calc. | pH 7.5 | Open record: factor Xa variant I16L, methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide |
| factor Xavariant I16L | Homo sapiens | methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide20 mM Hepes; 0.15 M NaCl; 2 mM CaCl2; Table 1 | 33 | 0.29 | 110 calc. | pH 7.5 | Open record: factor Xa variant I16L, methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide |
| factor Xavariant I16L | Homo sapiens | methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide20 mM Hepes; 0.15 M NaCl; 2 mM CaCl2; in the text | 70 | — | — | pH 7.5 | Open record: factor Xa variant I16L, methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide |
| factor Xavariant I16L | Homo sapiens | methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide20 mM Hepes; 0.15 M NaCl; 2 mM CaCl2; in the text | 51 | — | — | pH 7.5 | Open record: factor Xa variant I16L, methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide |
| factor Xavariant I16L | Homo sapiens | methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilidefree FXa with 500 mM Na+ (high NaCl); no FVa; in the text | — | 0.43 | — | pH 7.5 | Open record: factor Xa variant I16L, methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide |
| factor Xavariant I16L | Homo sapiens | methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilidein the text | — | 0.156 | — | pH 7.5 | Open record: factor Xa variant I16L, methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide |
| factor Xavariant I16L | Homo sapiens | prethrombin-1 | 6.65 | 0.0057 | 1.2 × 10³ | pH 7.5 | Open record: factor Xa variant I16L, prethrombin-1 |
| factor Xavariant I16L | Homo sapiens | prothrombin | 10.32 | 3.1 × 10⁻⁴ | 3.3 × 10⁴ | pH 7.5 | Open record: factor Xa variant I16L, prothrombin |
| factor Xavariant V17A | Homo sapiens | methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilidefree FXa; 6.0 nM FXa with SpecXa 10-500 μM | 73 | 1.38 | 53 calc. | pH 7.5 | Open record: factor Xa variant V17A, methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide |
| factor Xavariant V17A | Homo sapiens | methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide5.0 nM FXa with SpecXa 10-500 μM | 91 | 0.49 | 190 calc. | pH 7.5 | Open record: factor Xa variant V17A, methoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide |
| factor Xavariant V17A | Homo sapiens | prethrombin-1 | 6.47 | 0.0069 | 900 | pH 7.5 | Open record: factor Xa variant V17A, prethrombin-1 |
| factor Xavariant V17A | Homo sapiens | prothrombin | 14.78 | 4.7 × 10⁻⁴ | 3.1 × 10⁴ | pH 7.5 | Open record: factor Xa variant V17A, prothrombin |