CatLogenzyme kinetics catalog

EC 3.4.21.6: coagulation factor Xa

HydrolasesOpen in Browse

CatLog read 85 records for EC 3.4.21.6 from 4 papers, for 2 organisms. Names in these papers: factor Xa, activated factor X (FXa).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 20 records for EC 3.4.21.6 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.21.6 in Browse.

Papers

PaperOrganismsRecords
The elusive role of the potential factor X cation-binding exosite-1 in substrate and inhibitor interactionsHomo sapiens38
The conformational switch from the factor X zymogen to protease state mediates exosite expression and prothrombinase assemblyHomo sapiens20
Active-site mapping of bovine and human blood coagulation serine proteases using synthetic peptide 4-nitroanilide and thio ester substratesBos taurus14
Analysis of the kinetics of prothrombin activation and evidence that two equilibrating forms of prothrombinase are involved in the processHomo sapiens13

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
activated factor X (FXa)Homo sapiensbenzyl-Ile-Glu-Gly-Arg-p-nitroanilide2110.318664 calc.PMID 14583605Open
activated factor X (FXa)Homo sapiensbenzyloxycarbonyl-D-Arg-Gly-Arg-p-nitroanilide2540.0614.2 × 10³ calc.PMID 14583605Open
activated factor X (FXa)Homo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ile-Leu-Gly-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——3.6PMID 14583605Open
activated factor X (FXa)Homo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ser-Ala-Gly-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——12PMID 14583605Open
activated factor X (FXa)Homo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ser-Leu-Glu-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——17PMID 14583605Open
activated factor X (FXa)Homo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ser-Leu-Thr-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——30PMID 14583605Open
activated factor X (FXa)Homo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ser-Val-Gly-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——15PMID 14583605Open
activated factor X (FXa)Homo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Thr-Leu-Gly-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——15PMID 14583605Open
activated factor X (FXa)Homo sapiensprothrombinno FVa; 0.2 nM FXa0.00653.06 × 10⁻⁴21 calc.pH 7.4, 25 °CPMID 14583605Open
activated factor X (FXa)Homo sapiensprothrombin0.1 nM FXa5.78.6 × 10⁻⁵6.6 × 10⁴ calc.pH 7.4, 25 °CPMID 14583605Open
activated factor X (FXa)variant E36K/E37K/E39KHomo sapiensbenzyl-Ile-Glu-Gly-Arg-p-nitroanilide1960.339578 calc.PMID 14583605Open
activated factor X (FXa)variant E36K/E37K/E39KHomo sapiensbenzyloxycarbonyl-D-Arg-Gly-Arg-p-nitroanilide2150.0593.6 × 10³ calc.PMID 14583605Open
activated factor X (FXa)variant E36K/E37K/E39KHomo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ile-Leu-Gly-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——7.6PMID 14583605Open
activated factor X (FXa)variant E36K/E37K/E39KHomo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ser-Ala-Gly-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——22PMID 14583605Open
activated factor X (FXa)variant E36K/E37K/E39KHomo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ser-Leu-Glu-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——36PMID 14583605Open
activated factor X (FXa)variant E36K/E37K/E39KHomo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ser-Leu-Thr-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——26PMID 14583605Open
activated factor X (FXa)variant E36K/E37K/E39KHomo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ser-Val-Gly-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——19PMID 14583605Open
activated factor X (FXa)variant E36K/E37K/E39KHomo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Thr-Leu-Gly-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——23PMID 14583605Open
activated factor X (FXa)variant E36K/E37K/E39KHomo sapiensprothrombinno FVa; 0.2 nM FXa6.9 × 10⁻⁴2.99 × 10⁻⁴2.3 calc.pH 7.4, 25 °CPMID 14583605Open
activated factor X (FXa)variant E36K/E37K/E39KHomo sapiensprothrombin0.1 nM FXa0.64.5 × 10⁻⁵1.3 × 10⁴ calc.pH 7.4, 25 °CPMID 14583605Open
activated factor X (FXa)variant E36Q/E37Q/E39QHomo sapiensbenzyl-Ile-Glu-Gly-Arg-p-nitroanilide2000.333600 calc.PMID 14583605Open
activated factor X (FXa)variant E36Q/E37Q/E39QHomo sapiensbenzyloxycarbonyl-D-Arg-Gly-Arg-p-nitroanilide2140.0524.1 × 10³ calc.PMID 14583605Open
activated factor X (FXa)variant E36Q/E37Q/E39QHomo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ile-Leu-Gly-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——7.6PMID 14583605Open
activated factor X (FXa)variant E36Q/E37Q/E39QHomo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ser-Ala-Gly-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——19PMID 14583605Open
activated factor X (FXa)variant E36Q/E37Q/E39QHomo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ser-Leu-Glu-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——21PMID 14583605Open
activated factor X (FXa)variant E36Q/E37Q/E39QHomo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ser-Leu-Thr-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——27PMID 14583605Open
activated factor X (FXa)variant E36Q/E37Q/E39QHomo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Ser-Val-Gly-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——17PMID 14583605Open
activated factor X (FXa)variant E36Q/E37Q/E39QHomo sapiensortho-aminobenzoyl-Val-Gln-Phe-Arg-Thr-Leu-Gly-Asp-Gln-N-(2,4-dinitrophenyl)ethylenediamine——19PMID 14583605Open
activated factor X (FXa)variant E36Q/E37Q/E39QHomo sapiensprothrombinno FVa; 0.2 nM FXa7.4 × 10⁻⁴2.93 × 10⁻⁴2.5 calc.pH 7.4, 25 °CPMID 14583605Open
activated factor X (FXa)variant E36Q/E37Q/E39QHomo sapiensprothrombin0.1 nM FXa0.65.7 × 10⁻⁵1.1 × 10⁴ calc.pH 7.4, 25 °CPMID 14583605Open
activated factor X (FXa)variant E74K/E76K/E77KHomo sapiensbenzyl-Ile-Glu-Gly-Arg-p-nitroanilide1390.739188 calc.PMID 14583605Open
activated factor X (FXa)variant E74K/E76K/E77KHomo sapiensbenzyloxycarbonyl-D-Arg-Gly-Arg-p-nitroanilide2690.1122.40 × 10³ calc.PMID 14583605Open
activated factor X (FXa)variant E74K/E76K/E77KHomo sapiensprothrombinno FVa; 0.2 nM FXa0.00535.19 × 10⁻⁴10 calc.pH 7.4, 25 °CPMID 14583605Open
activated factor X (FXa)variant E74K/E76K/E77KHomo sapiensprothrombin0.1 nM FXa7.31.11 × 10⁻⁴6.6 × 10⁴ calc.pH 7.4, 25 °CPMID 14583605Open
activated factor X (FXa)variant E74Q/E76Q/E77QHomo sapiensbenzyl-Ile-Glu-Gly-Arg-p-nitroanilide1480.529280 calc.PMID 14583605Open
activated factor X (FXa)variant E74Q/E76Q/E77QHomo sapiensbenzyloxycarbonyl-D-Arg-Gly-Arg-p-nitroanilide2700.0942.9 × 10³ calc.PMID 14583605Open
activated factor X (FXa)variant E74Q/E76Q/E77QHomo sapiensprothrombinno FVa; 0.2 nM FXa0.00451.81 × 10⁻⁴25 calc.pH 7.4, 25 °CPMID 14583605Open
activated factor X (FXa)variant E74Q/E76Q/E77QHomo sapiensprothrombin0.1 nM FXa10.51.29 × 10⁻⁴8.14 × 10⁴ calc.pH 7.4, 25 °CPMID 14583605Open
factor XaBos taurusN-benzyloxycarbonyl-L-alanyl-glycyl-L-arginine 4-nitroanilide955.218pH 7.5, 25 °CPMID 6370301Open
factor XaBos taurusN-benzyloxycarbonyl-L-alanyl-L-phenylalanyl-L-arginine 4-nitroanilide1.80.247.5pH 7.5, 25 °CPMID 6370301Open
factor XaBos taurusN-benzyloxycarbonyl-L-alpha-glutamyl-glycyl-L-arginine 4-nitroanilide4331139pH 7.5, 25 °CPMID 6370301Open
factor XaBos taurusN-benzyloxycarbonyl-L-alpha-glutamyl-L-phenylalanyl-L-arginine 4-nitroanilide10.175.9pH 7.5, 25 °CPMID 6370301Open
factor XaBos taurusN-benzyloxycarbonyl-L-asparaginyl-glycyl-L-arginine 4-nitroanilide953.428pH 7.5, 25 °CPMID 6370301Open
factor XaBos taurusN-benzyloxycarbonyl-L-asparaginyl-L-phenylalanyl-L-arginine 4-nitroanilide202.19.5pH 7.5, 25 °CPMID 6370301Open
factor XaBos taurusN-benzyloxycarbonyl-L-lysyl-glycyl-L-arginine 4-nitroanilide786.213pH 7.5, 25 °CPMID 6370301Open
factor XaBos taurusN-benzyloxycarbonyl-L-lysyl-L-phenylalanyl-L-arginine 4-nitroanilide0.580.242.4pH 7.5, 25 °CPMID 6370301Open
factor XaBos taurusN-benzyloxycarbonyl-L-phenylalanyl-glycyl-L-arginine 4-nitroanilide1524.534pH 7.5, 25 °CPMID 6370301Open
factor XaBos taurusN-benzyloxycarbonyl-L-phenylalanyl-L-phenylalanyl-L-arginine 4-nitroanilide0.430.03811pH 7.5, 25 °CPMID 6370301Open
factor XaBos taurusN-benzyloxycarbonyl-L-prolyl-glycyl-L-arginine 4-nitroanilide121167.8pH 7.5, 25 °CPMID 6370301Open
factor XaBos taurusN-benzyloxycarbonyl-L-prolyl-L-phenylalanyl-L-arginine 4-nitroanilide1.10.991.1pH 7.5, 25 °CPMID 6370301Open
factor XaBos taurusN-benzyloxycarbonyl-L-seryl-glycyl-L-arginine 4-nitroanilide——29pH 7.5, 25 °CPMID 6370301Open
factor XaBos taurusN-benzyloxycarbonyl-L-seryl-L-phenylalanyl-L-arginine 4-nitroanilide7.50.948pH 7.5, 25 °CPMID 6370301Open
factor XaHomo sapiensfragment 1.2:prethrombin-2with factor Va (prothrombinase): 0.02 nM factor Xa1145.77 × 10⁻⁴1.98 × 10⁵PMID 12496269Open
factor XaHomo sapiensfragment 1.2:prethrombin-2without factor Va: 20 nM factor Xa0.00577.8 × 10⁻⁴7.3PMID 12496269Open
factor XaHomo sapiensmeizothrombinwith factor Va (prothrombinase): 0.02 nM factor Xa1506.53 × 10⁻⁴2.3 × 10⁵PMID 12496269Open
factor XaHomo sapiensmeizothrombinwithout factor Va: 20 nM factor Xa6.399.45 × 10⁻⁴6.76 × 10³PMID 12496269Open
factor XaHomo sapiensmethoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilidefree FXa; 2.0 nM FXa with SpecXa 10-500 μM1400.0721.9 × 10³ calc.pH 7.5PMID 18460471Open
factor XaHomo sapiensmethoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide5.0 nM FXa with SpecXa 10-500 μM720.17420 calc.pH 7.5PMID 18460471Open
factor XaHomo sapiensmethoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilidefree FXa; 2.0 nM FXa with SpecXa 10-500 μM2100.0892.4 × 10³ calc.pH 7.5PMID 18460471Open
factor XaHomo sapiensmethoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide5.0 nM FXa with SpecXa 10-500 μM1400.131.1 × 10³ calc.pH 7.5PMID 18460471Open
factor XaHomo sapiensprethrombin-1plasma-derived factor X purified from human plasma18.130.00543.3 × 10³pH 7.5PMID 18460471Open
factor XaHomo sapiensprethrombin-1recombinant factor X expressed in HEK293 cells17.50.00523.4 × 10³pH 7.5PMID 18460471Open
factor XaHomo sapiensprothrombinplasma-derived factor X purified from human plasma40.44.2 × 10⁻⁴9.6 × 10⁴pH 7.5PMID 18460471Open
factor XaHomo sapiensprothrombinrecombinant factor X expressed in HEK293 cells32.283.5 × 10⁻⁴9.2 × 10⁴pH 7.5PMID 18460471Open
factor XaHomo sapiensR155A/R284A/R271A prothrombinwith factor Va (prothrombinase): 0.02 nM factor Xa1103.55 × 10⁻⁴3.09 × 10⁵PMID 12496269Open
factor XaHomo sapiensR155A/R284A/R271A prothrombinwithout factor Va: 20 nM factor Xa0.00684.5 × 10⁻⁴15PMID 12496269Open
factor XaHomo sapiensR155A/R284A/R271A/S525C prothrombin labeled with fluorescein at Cys5251909.24 × 10⁻⁴2.1 × 10⁵ calc.PMID 12496269Open
factor XaHomo sapiensR155A/R284A/R320A prothrombinwith factor Va (prothrombinase): 0.02 nM factor Xa1143.93 × 10⁻⁴2.91 × 10⁵PMID 12496269Open
factor XaHomo sapiensR155A/R284A/R320A prothrombinwithout factor Va: 20 nM factor Xa0.3245.04 × 10⁻⁴640PMID 12496269Open
factor XaHomo sapiensrecombinant wild-type human prothrombinwith factor Va (prothrombinase): 0.02 nM factor Xa1084.11 × 10⁻⁴2.62 × 10⁵PMID 12496269Open
factor XaHomo sapiensrecombinant wild-type human prothrombinwithout factor Va: 20 nM factor Xa0.00456.82 × 10⁻⁴6.6PMID 12496269Open
factor XaHomo sapiensS525C prothrombin labeled with fluorescein at Cys525competition with rP2-II1073.86 × 10⁻⁴2.77 × 10⁵ calc.PMID 12496269Open
factor XaHomo sapiensS525C prothrombin labeled with fluorescein at Cys525competition with rMZ-II1275.57 × 10⁻⁴2.28 × 10⁵ calc.PMID 12496269Open
factor Xavariant I16LHomo sapiensmethoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilidefree FXa; 20 mM Hepes; 0.15 M NaCl; 2 mM CaCl2; Table 1581.1550 calc.pH 7.5PMID 18460471Open
factor Xavariant I16LHomo sapiensmethoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide20 mM Hepes; 0.15 M NaCl; 2 mM CaCl2; Table 1330.29110 calc.pH 7.5PMID 18460471Open
factor Xavariant I16LHomo sapiensmethoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide20 mM Hepes; 0.15 M NaCl; 2 mM CaCl2; in the text70——pH 7.5PMID 18460471Open
factor Xavariant I16LHomo sapiensmethoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide20 mM Hepes; 0.15 M NaCl; 2 mM CaCl2; in the text51——pH 7.5PMID 18460471Open
factor Xavariant I16LHomo sapiensmethoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilidefree FXa with 500 mM Na+ (high NaCl); no FVa; in the text—0.43—pH 7.5PMID 18460471Open
factor Xavariant I16LHomo sapiensmethoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilidein the text—0.156—pH 7.5PMID 18460471Open
factor Xavariant I16LHomo sapiensprethrombin-16.650.00571.2 × 10³pH 7.5PMID 18460471Open
factor Xavariant I16LHomo sapiensprothrombin10.323.1 × 10⁻⁴3.3 × 10⁴pH 7.5PMID 18460471Open
factor Xavariant V17AHomo sapiensmethoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilidefree FXa; 6.0 nM FXa with SpecXa 10-500 μM731.3853 calc.pH 7.5PMID 18460471Open
factor Xavariant V17AHomo sapiensmethoxycarbonylcyclohexylglycylglycylarginine-p-nitroanilide5.0 nM FXa with SpecXa 10-500 μM910.49190 calc.pH 7.5PMID 18460471Open
factor Xavariant V17AHomo sapiensprethrombin-16.470.0069900pH 7.5PMID 18460471Open
factor Xavariant V17AHomo sapiensprothrombin14.784.7 × 10⁻⁴3.1 × 10⁴pH 7.5PMID 18460471Open