Values CatLog read from this paper

Molecular basis of the substrate specificity and the catalytic mechanism of citramalate synthase from Leptospira interrogans

PMID 18498255doi:10.1042/bj20080242Open in Browse

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CatLog read 42 records (126 values) from this paper: citramalate synthase (LiCMS) from Leptospira interrogans.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.3.3.21

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
citramalate synthase (LiCMS)	Leptospira interrogans	2-oxobutanoate	0.09	0.695	0.13	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS), 2-oxobutanoate
citramalate synthase (LiCMS)	Leptospira interrogans	acetyl-CoA	10.3	1.118	9.2	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS), acetyl-CoA
citramalate synthase (LiCMS)	Leptospira interrogans	acetyl-CoAN-terminal catalytic domain (LiCMSN); residues 1-330	0.011	5.273	0.0022	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS), acetyl-CoA
citramalate synthase (LiCMS)	Leptospira interrogans	glyoxylate	0.51	1.47	0.35	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS), glyoxylate
citramalate synthase (LiCMS)	Leptospira interrogans	pyruvate	9.13	0.06	150	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS), pyruvate
citramalate synthase (LiCMS)	Leptospira interrogans	pyruvateN-terminal catalytic domain (LiCMSN); residues 1-330	0.004	0.15	0.025	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS), pyruvate
citramalate synthase (LiCMS)variant D17A	Leptospira interrogans	pyruvate	0.029	2.036	0.014	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant D17A, pyruvate
citramalate synthase (LiCMS)variant D17N	Leptospira interrogans	pyruvate	0.019	0.263	0.073	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant D17N, pyruvate
citramalate synthase (LiCMS)variant D304A	Leptospira interrogans	acetyl-CoA	0.62	5.851	0.11	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant D304A, acetyl-CoA
citramalate synthase (LiCMS)variant D304A	Leptospira interrogans	pyruvate	0.32	0.153	2.1	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant D304A, pyruvate
citramalate synthase (LiCMS)variant E146D	Leptospira interrogans	acetyl-CoA	0.021	1.511	0.014	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant E146D, acetyl-CoA
citramalate synthase (LiCMS)variant E146Q	Leptospira interrogans	acetyl-CoA	0.024	2.391	0.01	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant E146Q, acetyl-CoA
citramalate synthase (LiCMS)variant F83A	Leptospira interrogans	acetyl-CoA	0.085	5.186	0.016	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant F83A, acetyl-CoA
citramalate synthase (LiCMS)variant L104V	Leptospira interrogans	2-oxobutanoate	0.03	0.471	0.068	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L104V, 2-oxobutanoate
citramalate synthase (LiCMS)variant L104V	Leptospira interrogans	3-methyl-2-oxobutanoate	0.03	0.253	0.13	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L104V, 3-methyl-2-oxobutanoate
citramalate synthase (LiCMS)variant L104V	Leptospira interrogans	acetyl-CoA	3.9	3.028	1.3	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L104V, acetyl-CoA
citramalate synthase (LiCMS)variant L104V	Leptospira interrogans	glyoxylate	0.6	2.898	0.19	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L104V, glyoxylate
citramalate synthase (LiCMS)variant L104V	Leptospira interrogans	pyruvate	2.7	0.105	25	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L104V, pyruvate
citramalate synthase (LiCMS)variant L311A	Leptospira interrogans	acetyl-CoA	1.7	8.921	0.19	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L311A, acetyl-CoA
citramalate synthase (LiCMS)variant L311A	Leptospira interrogans	pyruvate	1.7	1.272	1.4	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L311A, pyruvate
citramalate synthase (LiCMS)variant L81A	Leptospira interrogans	2-oxobutanoate	0.05	0.465	0.1	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L81A, 2-oxobutanoate
citramalate synthase (LiCMS)variant L81A	Leptospira interrogans	3-methyl-2-oxobutanoate	0.018	0.098	0.19	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L81A, 3-methyl-2-oxobutanoate
citramalate synthase (LiCMS)variant L81A	Leptospira interrogans	acetyl-CoA	0.7	1.137	0.58	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L81A, acetyl-CoA
citramalate synthase (LiCMS)variant L81A	Leptospira interrogans	glyoxylate	0.19	10.61	0.018	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L81A, glyoxylate
citramalate synthase (LiCMS)variant L81A	Leptospira interrogans	pyruvate	0.58	0.282	2.1	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L81A, pyruvate
citramalate synthase (LiCMS)variant L81V	Leptospira interrogans	2-oxobutanoate	0.014	2.924	0.0048	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L81V, 2-oxobutanoate
citramalate synthase (LiCMS)variant L81V	Leptospira interrogans	acetyl-CoA	1.1	1.214	0.92	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L81V, acetyl-CoA
citramalate synthase (LiCMS)variant L81V	Leptospira interrogans	glyoxylate	0.21	7.225	0.03	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L81V, glyoxylate
citramalate synthase (LiCMS)variant L81V	Leptospira interrogans	pyruvate	0.9	0.198	4.5	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant L81V, pyruvate
citramalate synthase (LiCMS)variant N310A	Leptospira interrogans	acetyl-CoA	5.9	2.409	2.5	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant N310A, acetyl-CoA
citramalate synthase (LiCMS)variant N310A	Leptospira interrogans	pyruvate	3.8	0.17	22	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant N310A, pyruvate
citramalate synthase (LiCMS)variant T179A	Leptospira interrogans	pyruvate	0.049	0.979	0.05	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant T179A, pyruvate
citramalate synthase (LiCMS)variant Y144L	Leptospira interrogans	2-oxobutanoate	0.005	12.61	5 × 10⁻⁴	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant Y144L, 2-oxobutanoate
citramalate synthase (LiCMS)variant Y144L	Leptospira interrogans	3-methyl-2-oxobutanoate	0.023	0.151	0.15	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant Y144L, 3-methyl-2-oxobutanoate
citramalate synthase (LiCMS)variant Y144L	Leptospira interrogans	acetyl-CoA	0.017	1.904	0.01	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant Y144L, acetyl-CoA
citramalate synthase (LiCMS)variant Y144L	Leptospira interrogans	glyoxylate	0.005	3.498	0.0017	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant Y144L, glyoxylate
citramalate synthase (LiCMS)variant Y144L	Leptospira interrogans	pyruvate	0.12	15.53	0.0077	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant Y144L, pyruvate
citramalate synthase (LiCMS)variant Y144V	Leptospira interrogans	2-oxobutanoate	0.62	6.519	0.096	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant Y144V, 2-oxobutanoate
citramalate synthase (LiCMS)variant Y144V	Leptospira interrogans	3-methyl-2-oxobutanoate	0.062	52.85	0.00117	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant Y144V, 3-methyl-2-oxobutanoate
citramalate synthase (LiCMS)variant Y144V	Leptospira interrogans	acetyl-CoA	0.6	1.691	0.34	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant Y144V, acetyl-CoA
citramalate synthase (LiCMS)variant Y144V	Leptospira interrogans	glyoxylate	0.03	1.023	0.034	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant Y144V, glyoxylate
citramalate synthase (LiCMS)variant Y144V	Leptospira interrogans	pyruvate	1.7	6.859	0.24	pH 7.7, 37 °C	Open record: citramalate synthase (LiCMS) variant Y144V, pyruvate