EC 2.3.3.21: (R)-citramalate synthase
TransferasesOpen in Browse
CatLog read 56 records for EC 2.3.3.21 from 2 papers, for 1 organism. Names in these papers: citramalate synthase (LiCMS).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 5 records for EC 2.3.3.21 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.3.3.21 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Molecular basis of the substrate specificity and the catalytic mechanism of citramalate synthase from Leptospira interrogans | Leptospira interrogans | 42 |
| Molecular basis of the inhibitor selectivity and insights into the feedback inhibition mechanism of citramalate synthase from Leptospira interrogans | Leptospira interrogans | 14 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| citramalate synthase (LiCMS) | Leptospira interrogans | 2-oxobutanoate | 0.09 | 0.695 | 0.13 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS), 2-oxobutanoate |
| citramalate synthase (LiCMS) | Leptospira interrogans | acetyl coenzyme A20 µM isoleucine (feedback inhibitor) | 10.6 | 2.272 | 4.6 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS), acetyl coenzyme A |
| citramalate synthase (LiCMS) | Leptospira interrogans | acetyl coenzyme A60 µM isoleucine (feedback inhibitor) | 11.1 | 4.246 | 2.6 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS), acetyl coenzyme A |
| citramalate synthase (LiCMS) | Leptospira interrogans | acetyl-CoA | 10.3 | 1.118 | 9.2 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS), acetyl-CoA |
| citramalate synthase (LiCMS) | Leptospira interrogans | acetyl-CoAN-terminal catalytic domain (LiCMSN); residues 1-330 | 0.011 | 5.273 | 0.0022 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS), acetyl-CoA |
| citramalate synthase (LiCMS) | Leptospira interrogans | glyoxylate | 0.51 | 1.47 | 0.35 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS), glyoxylate |
| citramalate synthase (LiCMS) | Leptospira interrogans | pyruvate | 9.13 | 0.06 | 150 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS), pyruvate |
| citramalate synthase (LiCMS) | Leptospira interrogans | pyruvateN-terminal catalytic domain (LiCMSN); residues 1-330 | 0.004 | 0.15 | 0.025 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS), pyruvate |
| citramalate synthase (LiCMS) | Leptospira interrogans | pyruvate1 µM isoleucine (feedback inhibitor) | 9.2 | 0.094 | 98 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS), pyruvate |
| citramalate synthase (LiCMS) | Leptospira interrogans | pyruvate4 µM isoleucine (feedback inhibitor) | 9.3 | 0.178 | 52 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS), pyruvate |
| citramalate synthase (LiCMS)variant D17A | Leptospira interrogans | pyruvate | 0.029 | 2.036 | 0.014 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant D17A, pyruvate |
| citramalate synthase (LiCMS)variant D17N | Leptospira interrogans | pyruvate | 0.019 | 0.263 | 0.073 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant D17N, pyruvate |
| citramalate synthase (LiCMS)variant D304A | Leptospira interrogans | acetyl-CoA | 0.62 | 5.851 | 0.11 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant D304A, acetyl-CoA |
| citramalate synthase (LiCMS)variant D304A | Leptospira interrogans | pyruvate | 0.32 | 0.153 | 2.1 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant D304A, pyruvate |
| citramalate synthase (LiCMS)variant D431A | Leptospira interrogans | acetyl coenzyme A | 2 | 0.629 | 3.2 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS) variant D431A, acetyl coenzyme A |
| citramalate synthase (LiCMS)variant E146D | Leptospira interrogans | acetyl-CoA | 0.021 | 1.511 | 0.014 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant E146D, acetyl-CoA |
| citramalate synthase (LiCMS)variant E146Q | Leptospira interrogans | acetyl-CoA | 0.024 | 2.391 | 0.01 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant E146Q, acetyl-CoA |
| citramalate synthase (LiCMS)variant F83A | Leptospira interrogans | acetyl-CoA | 0.085 | 5.186 | 0.016 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant F83A, acetyl-CoA |
| citramalate synthase (LiCMS)variant H400A/H408A | Leptospira interrogans | acetyl coenzyme A | 9.1 | 1.554 | 5.9 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS) variant H400A/H408A, acetyl coenzyme A |
| citramalate synthase (LiCMS)variant I458A | Leptospira interrogans | acetyl coenzyme A | 0.7 | 0.838 | 0.8 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS) variant I458A, acetyl coenzyme A |
| citramalate synthase (LiCMS)variant L104V | Leptospira interrogans | 2-oxobutanoate | 0.03 | 0.471 | 0.068 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L104V, 2-oxobutanoate |
| citramalate synthase (LiCMS)variant L104V | Leptospira interrogans | 3-methyl-2-oxobutanoate | 0.03 | 0.253 | 0.13 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L104V, 3-methyl-2-oxobutanoate |
| citramalate synthase (LiCMS)variant L104V | Leptospira interrogans | acetyl-CoA | 3.9 | 3.028 | 1.3 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L104V, acetyl-CoA |
| citramalate synthase (LiCMS)variant L104V | Leptospira interrogans | glyoxylate | 0.6 | 2.898 | 0.19 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L104V, glyoxylate |
| citramalate synthase (LiCMS)variant L104V | Leptospira interrogans | pyruvate | 2.7 | 0.105 | 25 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L104V, pyruvate |
| citramalate synthase (LiCMS)variant L311A | Leptospira interrogans | acetyl-CoA | 1.7 | 8.921 | 0.19 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L311A, acetyl-CoA |
| citramalate synthase (LiCMS)variant L311A | Leptospira interrogans | pyruvate | 1.7 | 1.272 | 1.4 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L311A, pyruvate |
| citramalate synthase (LiCMS)variant L451V | Leptospira interrogans | acetyl coenzyme A | 2.4 | 1.711 | 1.4 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS) variant L451V, acetyl coenzyme A |
| citramalate synthase (LiCMS)variant L81A | Leptospira interrogans | 2-oxobutanoate | 0.05 | 0.465 | 0.1 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L81A, 2-oxobutanoate |
| citramalate synthase (LiCMS)variant L81A | Leptospira interrogans | 3-methyl-2-oxobutanoate | 0.018 | 0.098 | 0.19 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L81A, 3-methyl-2-oxobutanoate |
| citramalate synthase (LiCMS)variant L81A | Leptospira interrogans | acetyl-CoA | 0.7 | 1.137 | 0.58 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L81A, acetyl-CoA |
| citramalate synthase (LiCMS)variant L81A | Leptospira interrogans | glyoxylate | 0.19 | 10.61 | 0.018 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L81A, glyoxylate |
| citramalate synthase (LiCMS)variant L81A | Leptospira interrogans | pyruvate | 0.58 | 0.282 | 2.1 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L81A, pyruvate |
| citramalate synthase (LiCMS)variant L81V | Leptospira interrogans | 2-oxobutanoate | 0.014 | 2.924 | 0.0048 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L81V, 2-oxobutanoate |
| citramalate synthase (LiCMS)variant L81V | Leptospira interrogans | acetyl-CoA | 1.1 | 1.214 | 0.92 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L81V, acetyl-CoA |
| citramalate synthase (LiCMS)variant L81V | Leptospira interrogans | glyoxylate | 0.21 | 7.225 | 0.03 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L81V, glyoxylate |
| citramalate synthase (LiCMS)variant L81V | Leptospira interrogans | pyruvate | 0.9 | 0.198 | 4.5 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant L81V, pyruvate |
| citramalate synthase (LiCMS)variant N310A | Leptospira interrogans | acetyl-CoA | 5.9 | 2.409 | 2.5 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant N310A, acetyl-CoA |
| citramalate synthase (LiCMS)variant N310A | Leptospira interrogans | pyruvate | 3.8 | 0.17 | 22 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant N310A, pyruvate |
| citramalate synthase (LiCMS)variant P493A | Leptospira interrogans | acetyl coenzyme A | 4 | 0.743 | 5.4 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS) variant P493A, acetyl coenzyme A |
| citramalate synthase (LiCMS)variant Q495A | Leptospira interrogans | acetyl coenzyme A | 3.7 | 0.712 | 5.2 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS) variant Q495A, acetyl coenzyme A |
| citramalate synthase (LiCMS)variant T179A | Leptospira interrogans | pyruvate | 0.049 | 0.979 | 0.05 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant T179A, pyruvate |
| citramalate synthase (LiCMS)variant T464A | Leptospira interrogans | acetyl coenzyme A | 2.4 | 0.626 | 3.8 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS) variant T464A, acetyl coenzyme A |
| citramalate synthase (LiCMS)variant V468A | Leptospira interrogans | acetyl coenzyme A | 5 | 1.116 | 4.5 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS) variant V468A, acetyl coenzyme A |
| citramalate synthase (LiCMS)variant Y144L | Leptospira interrogans | 2-oxobutanoate | 0.005 | 12.61 | 5 × 10⁻⁴ | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant Y144L, 2-oxobutanoate |
| citramalate synthase (LiCMS)variant Y144L | Leptospira interrogans | 3-methyl-2-oxobutanoate | 0.023 | 0.151 | 0.15 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant Y144L, 3-methyl-2-oxobutanoate |
| citramalate synthase (LiCMS)variant Y144L | Leptospira interrogans | acetyl-CoA | 0.017 | 1.904 | 0.01 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant Y144L, acetyl-CoA |
| citramalate synthase (LiCMS)variant Y144L | Leptospira interrogans | glyoxylate | 0.005 | 3.498 | 0.0017 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant Y144L, glyoxylate |
| citramalate synthase (LiCMS)variant Y144L | Leptospira interrogans | pyruvate | 0.12 | 15.53 | 0.0077 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant Y144L, pyruvate |
| citramalate synthase (LiCMS)variant Y144V | Leptospira interrogans | 2-oxobutanoate | 0.62 | 6.519 | 0.096 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant Y144V, 2-oxobutanoate |
| citramalate synthase (LiCMS)variant Y144V | Leptospira interrogans | 3-methyl-2-oxobutanoate | 0.062 | 52.85 | 0.00117 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant Y144V, 3-methyl-2-oxobutanoate |
| citramalate synthase (LiCMS)variant Y144V | Leptospira interrogans | acetyl-CoA | 0.6 | 1.691 | 0.34 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant Y144V, acetyl-CoA |
| citramalate synthase (LiCMS)variant Y144V | Leptospira interrogans | glyoxylate | 0.03 | 1.023 | 0.034 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant Y144V, glyoxylate |
| citramalate synthase (LiCMS)variant Y144V | Leptospira interrogans | pyruvate | 1.7 | 6.859 | 0.24 | pH 7.7, 37 °C | PMID 18498255 | Open record: citramalate synthase (LiCMS) variant Y144V, pyruvate |
| citramalate synthase (LiCMS)variant Y430L | Leptospira interrogans | acetyl coenzyme A | 4.5 | 1.097 | 4.1 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS) variant Y430L, acetyl coenzyme A |
| citramalate synthase (LiCMS)variant Y454A | Leptospira interrogans | acetyl coenzyme A | 0.6 | 0.803 | 0.74 | pH 7.7 | PMID 19351325 | Open record: citramalate synthase (LiCMS) variant Y454A, acetyl coenzyme A |