Values CatLog read from this paper
Structural principles of the broad substrate specificity of Thermoactinomyces vulgaris carboxypeptidase T--role of amino acid residues at positions 260 and 262
PMID 18515300doi:10.1093/protein/gzn031Open in Browse
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CatLog read 14 records (34 values) from this paper: carboxypeptidase T from Thermoactinomyces vulgaris.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| carboxypeptidase Tvariant D260G | Thermoactinomyces vulgaris | 2,4-dinitrophenyl-alanyl-alanyl-arginine | — | — | 0.346 | pH 7.5, 37 °C | Open record: carboxypeptidase T variant D260G, 2,4-dinitrophenyl-alanyl-alanyl-arginine |
| carboxypeptidase Tvariant D260G | Thermoactinomyces vulgaris | benzyloxycarbonyl-alanyl-alanyl-glutamic acid | 7.2 | 0.18 | 40 | pH 7.5, 25 °C | Open record: carboxypeptidase T variant D260G, benzyloxycarbonyl-alanyl-alanyl-glutamic acid |
| carboxypeptidase Tvariant D260G | Thermoactinomyces vulgaris | benzyloxycarbonyl-alanyl-alanyl-leucine | 4.1 | 0.016 | 282 | pH 7.5, 25 °C | Open record: carboxypeptidase T variant D260G, benzyloxycarbonyl-alanyl-alanyl-leucine |
| carboxypeptidase Tvariant D260G/T262I | Thermoactinomyces vulgaris | 2,4-dinitrophenyl-alanyl-alanyl-arginine | — | — | 0.132 | pH 7.5, 37 °C | Open record: carboxypeptidase T variant D260G/T262I, 2,4-dinitrophenyl-alanyl-alanyl-arginine |
| carboxypeptidase Tvariant D260G/T262I | Thermoactinomyces vulgaris | benzyloxycarbonyl-alanyl-alanyl-glutamic acid | 8.5 | 0.3 | 28 | pH 7.5, 25 °C | Open record: carboxypeptidase T variant D260G/T262I, benzyloxycarbonyl-alanyl-alanyl-glutamic acid |
| carboxypeptidase Tvariant D260G/T262I | Thermoactinomyces vulgaris | benzyloxycarbonyl-alanyl-alanyl-leucine | 5.2 | 0.03 | 184 | pH 7.5, 25 °C | Open record: carboxypeptidase T variant D260G/T262I, benzyloxycarbonyl-alanyl-alanyl-leucine |
| carboxypeptidase Tvariant D260G/T262K | Thermoactinomyces vulgaris | 2,4-dinitrophenyl-alanyl-alanyl-arginine | 0.04 | 2.8 | 0.0168 | pH 7.5, 37 °C | Open record: carboxypeptidase T variant D260G/T262K, 2,4-dinitrophenyl-alanyl-alanyl-arginine |
| carboxypeptidase Tvariant D260G/T262K | Thermoactinomyces vulgaris | benzyloxycarbonyl-alanyl-alanyl-glutamic acid | 11.5 | 0.65 | 17.8 | pH 7.5, 25 °C | Open record: carboxypeptidase T variant D260G/T262K, benzyloxycarbonyl-alanyl-alanyl-glutamic acid |
| carboxypeptidase Tvariant D260G/T262K | Thermoactinomyces vulgaris | benzyloxycarbonyl-alanyl-alanyl-leucine | 1.6 | 0.033 | 55 | pH 7.5, 25 °C | Open record: carboxypeptidase T variant D260G/T262K, benzyloxycarbonyl-alanyl-alanyl-leucine |
| carboxypeptidase Tvariant D260G/T262R | Thermoactinomyces vulgaris | benzyloxycarbonyl-alanyl-alanyl-glutamic acid | 23.6 | 0.8 | 29.6 | pH 7.5, 25 °C | Open record: carboxypeptidase T variant D260G/T262R, benzyloxycarbonyl-alanyl-alanyl-glutamic acid |
| carboxypeptidase Tvariant D260G/T262R | Thermoactinomyces vulgaris | benzyloxycarbonyl-alanyl-alanyl-leucine | 5.6 | 0.45 | 12.5 | pH 7.5, 25 °C | Open record: carboxypeptidase T variant D260G/T262R, benzyloxycarbonyl-alanyl-alanyl-leucine |
| carboxypeptidase Tvariant G215S/A251G/T257A/D260G/T262D + Thr inserted between Asp253 and Leu254 (CPT6) | Thermoactinomyces vulgaris | 2,4-dinitrophenyl-alanyl-alanyl-arginine | — | — | 0.142 | pH 7.5, 37 °C | Open record: carboxypeptidase T variant G215S/A251G/T257A/D260G/T262D + Thr inserted between Asp253 and Leu254 (CPT6), 2,4-dinitrophenyl-alanyl-alanyl-arginine |
| carboxypeptidase Tvariant G215S/A251G/T257A/D260G/T262D + Thr inserted between Asp253 and Leu254 (CPT6) | Thermoactinomyces vulgaris | benzyloxycarbonyl-alanyl-alanyl-glutamic acid | — | — | 0.0375 | pH 7.5, 25 °C | Open record: carboxypeptidase T variant G215S/A251G/T257A/D260G/T262D + Thr inserted between Asp253 and Leu254 (CPT6), benzyloxycarbonyl-alanyl-alanyl-glutamic acid |
| carboxypeptidase Tvariant G215S/A251G/T257A/D260G/T262D + Thr inserted between Asp253 and Leu254 (CPT6) | Thermoactinomyces vulgaris | benzyloxycarbonyl-alanyl-alanyl-leucine | 7.1 | 0.75 | 9.7 | pH 7.5, 25 °C | Open record: carboxypeptidase T variant G215S/A251G/T257A/D260G/T262D + Thr inserted between Asp253 and Leu254 (CPT6), benzyloxycarbonyl-alanyl-alanyl-leucine |