Values CatLog read from this paper
Enzymatic activity and substrate specificity of mitogen-activated protein kinase p38alpha in different phosphorylation states
PMID 18669639doi:10.1074/jbc.m801703200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 27 records (69 values) from this paper: MKP5 (MAP kinase phosphatase 5), p38α (mitogen-activated protein kinase p38α), HePTP (hematopoietic protein-tyrosine phosphatase) and 2 more from Homo sapiens, Mus musculus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| HePTP (hematopoietic protein-tyrosine phosphatase) | Homo sapiens | ERK2 bisphosphorylated on the activation-loop Thr and Tyr | 1.23 | 0.00242 | 508 calc. | pH 7, 25 °C | Open record: HePTP (hematopoietic protein-tyrosine phosphatase), ERK2 bisphosphorylated on the activation-loop Thr and Tyr |
| HePTP (hematopoietic protein-tyrosine phosphatase) | Homo sapiens | p-nitrophenyl phosphate | 3.52 | 8.675 | 0.406 | pH 7, 25 °C | Open record: HePTP (hematopoietic protein-tyrosine phosphatase), p-nitrophenyl phosphate |
| HePTP (hematopoietic protein-tyrosine phosphatase) | Homo sapiens | p38α phosphorylated on both Thr-180 and Tyr-182 (bisphosphorylated mouse p38α)10 mM MgCl2 | 0.86 | 0.00286 | 300 | pH 7, 25 °C | Open record: HePTP (hematopoietic protein-tyrosine phosphatase), p38α phosphorylated on both Thr-180 and Tyr-182 (bisphosphorylated mouse p38α) |
| HePTP (hematopoietic protein-tyrosine phosphatase) | Homo sapiens | p38α phosphorylated on both Thr-180 and Tyr-182 (bisphosphorylated mouse p38α)without Mg2+ | 1.28 | 9.8 × 10⁻⁴ | 1.3 × 10³ calc. | pH 7, 25 °C | Open record: HePTP (hematopoietic protein-tyrosine phosphatase), p38α phosphorylated on both Thr-180 and Tyr-182 (bisphosphorylated mouse p38α) |
| HePTP (hematopoietic protein-tyrosine phosphatase) | Homo sapiens | p38α phosphorylated on Tyr-182 only | 0.27 | 0.00237 | 114 | pH 7, 25 °C | Open record: HePTP (hematopoietic protein-tyrosine phosphatase), p38α phosphorylated on Tyr-182 only |
| HePTP (hematopoietic protein-tyrosine phosphatase)variant ΔN43 | Homo sapiens | p-nitrophenyl phosphate | 5.1 | 9.5 | 0.5368 | pH 7, 25 °C | Open record: HePTP (hematopoietic protein-tyrosine phosphatase) variant ΔN43, p-nitrophenyl phosphate |
| MKP5 (MAP kinase phosphatase 5) | Homo sapiens | p-nitrophenyl phosphate | 0.22 | 17.68 | 0.0124 | pH 7, 25 °C | Open record: MKP5 (MAP kinase phosphatase 5), p-nitrophenyl phosphate |
| MKP5 (MAP kinase phosphatase 5) | Homo sapiens | p38α phosphorylated on both Thr-180 and Tyr-182 (bisphosphorylated mouse p38α) | 0.55 | 1.4 × 10⁻⁴ | 4.01 × 10³ | pH 7, 25 °C | Open record: MKP5 (MAP kinase phosphatase 5), p38α phosphorylated on both Thr-180 and Tyr-182 (bisphosphorylated mouse p38α) |
| MKP5 (MAP kinase phosphatase 5) | Homo sapiens | p38α phosphorylated on Thr-180 only | 0.42 | 3.3 × 10⁻⁴ | 1.28 × 10³ | pH 7, 25 °C | Open record: MKP5 (MAP kinase phosphatase 5), p38α phosphorylated on Thr-180 only |
| MKP5 (MAP kinase phosphatase 5) | Homo sapiens | p38α phosphorylated on Tyr-182 only | 0.78 | 4.6 × 10⁻⁴ | 1.69 × 10³ | pH 7, 25 °C | Open record: MKP5 (MAP kinase phosphatase 5), p38α phosphorylated on Tyr-182 only |
| MKP5 (MAP kinase phosphatase 5)variant ΔN121 | Homo sapiens | p-nitrophenyl phosphate | 0.132 | 21.1 | 0.00626 | pH 7, 25 °C | Open record: MKP5 (MAP kinase phosphatase 5) variant ΔN121, p-nitrophenyl phosphate |
| MKP5 (MAP kinase phosphatase 5)variant ΔN121 | Homo sapiens | p38α phosphorylated on both Thr-180 and Tyr-182 (bisphosphorylated mouse p38α) | 0.518 | 2.17 × 10⁻⁴ | 2.4 × 10³ | pH 7, 25 °C | Open record: MKP5 (MAP kinase phosphatase 5) variant ΔN121, p38α phosphorylated on both Thr-180 and Tyr-182 (bisphosphorylated mouse p38α) |
| MKP5 (MAP kinase phosphatase 5)variant ΔN319 | Homo sapiens | p-nitrophenyl phosphate | 0.07 | 36.4 | 0.0019 | pH 7, 25 °C | Open record: MKP5 (MAP kinase phosphatase 5) variant ΔN319, p-nitrophenyl phosphate |
| MKP5 (MAP kinase phosphatase 5)variant ΔN319 | Homo sapiens | p38α phosphorylated on both Thr-180 and Tyr-182 (bisphosphorylated mouse p38α) | 0.383 | 0.0596 | 6.4 | pH 7, 25 °C | Open record: MKP5 (MAP kinase phosphatase 5) variant ΔN319, p38α phosphorylated on both Thr-180 and Tyr-182 (bisphosphorylated mouse p38α) |
| p38α (mitogen-activated protein kinase p38α)variant p38α/pT (monophosphorylated on Thr-180) | Mus musculus | adenosine 5'-triphosphate | 0.062 | 1.669 | 0.0371 | pH 7, 25 °C | Open record: p38α (mitogen-activated protein kinase p38α) variant p38α/pT (monophosphorylated on Thr-180), adenosine 5'-triphosphate |
| p38α (mitogen-activated protein kinase p38α)variant p38α/pT (monophosphorylated on Thr-180) | Mus musculus | ATF2-(1-109) (activating transcription factor 2 residues 1-109) | 3.8 | 0.0201 | 190 | pH 7, 25 °C | Open record: p38α (mitogen-activated protein kinase p38α) variant p38α/pT (monophosphorylated on Thr-180), ATF2-(1-109) (activating transcription factor 2 residues 1-109) |
| p38α (mitogen-activated protein kinase p38α)variant p38α/pT (monophosphorylated on Thr-180) | Mus musculus | KRELVEPLTPSGEAPNQALLR (EGF receptor peptide, residues 661-681) | 6.99 | 2.8 | 2.5 | pH 7, 25 °C | Open record: p38α (mitogen-activated protein kinase p38α) variant p38α/pT (monophosphorylated on Thr-180), KRELVEPLTPSGEAPNQALLR (EGF receptor peptide, residues 661-681) |
| p38α (mitogen-activated protein kinase p38α)variant p38α/pTpY (bisphosphorylated on Thr-180 and Tyr-182) | Mus musculus | adenosine 5'-triphosphate | 0.562 | 0.212 | 2.65 | pH 7, 25 °C | Open record: p38α (mitogen-activated protein kinase p38α) variant p38α/pTpY (bisphosphorylated on Thr-180 and Tyr-182), adenosine 5'-triphosphate |
| p38α (mitogen-activated protein kinase p38α)variant p38α/pTpY (bisphosphorylated on Thr-180 and Tyr-182) | Mus musculus | ATF2-(1-109) (activating transcription factor 2 residues 1-109) | 4.7 | 0.00203 | 2.3 × 10³ | pH 7, 25 °C | Open record: p38α (mitogen-activated protein kinase p38α) variant p38α/pTpY (bisphosphorylated on Thr-180 and Tyr-182), ATF2-(1-109) (activating transcription factor 2 residues 1-109) |
| p38α (mitogen-activated protein kinase p38α)variant p38α/pTpY (bisphosphorylated on Thr-180 and Tyr-182) | Mus musculus | KRELVEPLTPSGEAPNQALLR (EGF receptor peptide, residues 661-681) | 31.6 | 0.656 | 48 | pH 7, 25 °C | Open record: p38α (mitogen-activated protein kinase p38α) variant p38α/pTpY (bisphosphorylated on Thr-180 and Tyr-182), KRELVEPLTPSGEAPNQALLR (EGF receptor peptide, residues 661-681) |
| p38α (mitogen-activated protein kinase p38α)variant p38α/pY (monophosphorylated on Tyr-182) | Mus musculus | ATF2-(1-109) (activating transcription factor 2 residues 1-109) | — | — | 2.497 | pH 7, 25 °C | Open record: p38α (mitogen-activated protein kinase p38α) variant p38α/pY (monophosphorylated on Tyr-182), ATF2-(1-109) (activating transcription factor 2 residues 1-109) |
| p38α (mitogen-activated protein kinase p38α)variant p38α/pY (monophosphorylated on Tyr-182) | Mus musculus | KRELVEPLTPSGEAPNQALLR (EGF receptor peptide, residues 661-681) | — | — | 0.0224 | pH 7, 25 °C | Open record: p38α (mitogen-activated protein kinase p38α) variant p38α/pY (monophosphorylated on Tyr-182), KRELVEPLTPSGEAPNQALLR (EGF receptor peptide, residues 661-681) |
| p38α (mitogen-activated protein kinase p38α) unphosphorylated p38α | Mus musculus | ATF2-(1-109) (activating transcription factor 2 residues 1-109) | — | — | 0.691 | pH 7, 25 °C | Open record: p38α (mitogen-activated protein kinase p38α) unphosphorylated p38α, ATF2-(1-109) (activating transcription factor 2 residues 1-109) |
| p38α (mitogen-activated protein kinase p38α) unphosphorylated p38α | Mus musculus | KRELVEPLTPSGEAPNQALLR (EGF receptor peptide, residues 661-681) | — | — | 0.0032 | pH 7, 25 °C | Open record: p38α (mitogen-activated protein kinase p38α) unphosphorylated p38α, KRELVEPLTPSGEAPNQALLR (EGF receptor peptide, residues 661-681) |
| PP2Cα (protein phosphatase 2Cα) | Homo sapiens | p-nitrophenyl phosphate | 0.75 | 0.585 | 1.28 | pH 7, 25 °C | Open record: PP2Cα (protein phosphatase 2Cα), p-nitrophenyl phosphate |
| PP2Cα (protein phosphatase 2Cα) | Homo sapiens | p38α phosphorylated on both Thr-180 and Tyr-182 (bisphosphorylated mouse p38α) | — | — | 65.5 | pH 7, 25 °C | Open record: PP2Cα (protein phosphatase 2Cα), p38α phosphorylated on both Thr-180 and Tyr-182 (bisphosphorylated mouse p38α) |
| PP2Cα (protein phosphatase 2Cα) | Homo sapiens | p38α phosphorylated on Thr-180 only | 2.75 | 0.0155 | 177 | pH 7, 25 °C | Open record: PP2Cα (protein phosphatase 2Cα), p38α phosphorylated on Thr-180 only |