Values CatLog read from this paper
Structural characterization and reversal of the natural organophosphate resistance of a D-type esterase, Saccharomyces cerevisiae S-formylglutathione hydrolase
PMID 18707125doi:10.1021/bi8010016Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 25 records (74 values) from this paper: S-formylglutathione hydrolase (ySFGH) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| S-formylglutathione hydrolase (ySFGH) | Saccharomyces cerevisiae | p-nitrophenyl acetateSorensen's buffer (0.067 M Na/K phosphate); 22 ± 2 °C | 1.8 | 0.4 | 4.5 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH), p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH) | Saccharomyces cerevisiae | p-nitrophenyl acetateSorensen's buffer; 22 °C; absorbance at 405 nm | 1.78 | 0.5 | 3.6 calc. | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH), p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH) | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 0.198 | 0.09 | 2.2 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH), p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60H/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 1.5 | 0.34 | 4.3 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60H/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60H/W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 3.63 | 0.13 | 28 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60H/W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60K/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.972 | 0.15 | 6.5 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60K/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60K/W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 1.23 | 0.03 | 42 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60K/W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60Q/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.525 | 0.1 | 5.3 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60Q/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60Q/W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 1.65 | 0.012 | 130 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60Q/W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60R/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.317 | 0.013 | 25 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60R/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60R/W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 0.43 | 0.018 | 23 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60R/W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60S | Saccharomyces cerevisiae | p-nitrophenyl acetate | 1.58 | 0.32 | 5 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60S, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60S | Saccharomyces cerevisiae | p-nitrophenyl acetateenzyme stock with 2-4 mM bME diluted 1:100 | 1.53 | 0.19 | 8 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60S, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60S | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 0.078 | 0.09 | 0.83 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60S, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60S/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.763 | 0.14 | 5.5 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60S/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60S/W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 1.97 | 0.019 | 103 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60S/W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60S/W197I | Saccharomyces cerevisiae | S-lactoylglutathione | 50 | 3 | 17 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant C60S/W197I, S-lactoylglutathione |
| S-formylglutathione hydrolase (ySFGH)variant L58H/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.0055 | 0.23 | 0.025 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant L58H/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant M162H | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.1 | 0.9 | 0.12 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant M162H, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant M162H | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 0.0032 | 0.14 | 0.023 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant M162H, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant M162H/C60S/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.028 | 2.6 | 0.012 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant M162H/C60S/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant M162H/C60S/W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 0.04 | 0.12 | 0.33 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant M162H/C60S/W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 1.05 | 0.054 | 20 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 1.92 | 0.04 | 48 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant W197I | Saccharomyces cerevisiae | S-lactoylglutathione | 120 | 3 | 33 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (ySFGH) variant W197I, S-lactoylglutathione |